Dept of Pathology

THE UNIVERSITY OF HONG KONG
Research and Scholarship 2004

DEPARTMENT OF PATHOLOGY

Researcher : Ang Chan EY

List of Research Outputs

Cheung A.N.Y., Szeto E.F., Ng K.M., Fong K.W., Ang Chan E.Y., Tsun O.K.L., Khoo U.S., Chan Y.K. and Ng W.Y., Atypical squamous cells of undetermined significance on cervical smears: follow-up study of an Asian screening population, Cancer Cytopathology. 2004, 102(2): 74-80.

Researcher : Beh SL

Project Title:	A study of the profile of causes of death in patients suspected to suffer from the Severe Acute Respiratory Syndrome (SARS) and its impact on subsequent clinical management
Investigator(s):	Dr. Beh P.S.L., Dr. Chung L.P., Dr. Lee K.C., Dr. Leung C.Y., Dr. Nicholls J.M., Dr. Pang S.W., Dr. Wong M.P.
Department:	Pathology
Source(s) of Funding:	SARS Research Fund
Start Date:	07/2003

Abstract:

To analyze the profile of the causes of death in patients with suspected SARS.

List of Research Outputs

Beh S.L., Death of the teaching autopsy: In Hong Kong teaching autopsies have been championed in public mortuaries, British Medical Journal. 2004, 328: 166.

Beh S.L., Medical Students Perception of the Autopsy, Presented at the 17th International Symposium of the Australian New Zealand Forensic Science Societies 2004 Wellington, New Zealand, April 2004.

Beh S.L. and Hau K.L., The development of forensic services in Hong Kong, History Book of the Indo-Pacific Association of Law, Medicine and Science (INPALMS), Edited by Salgado, M.S.L., Tatsuno, Y. & Tsunenari, S. Sri Lanka, The Medico-Legal Society of Sri Lanka, 2003, 149-152.

Beh S.L., 法醫學, 法醫學 - 全國高等學校醫學規劃教材主?：侯一平（四川大學), 高等教肓出版社, 2004.

Broadhurst R.G., Chan C.Y. and Beh S.L., Homicide-Suicide in Hong Kong, 1989-1998: A Preliminary Analysis, Crime and Its Control in People Republic of China. Hong Kong, Centre for Criminology, The University of Hong Kong, 2004, 326-331.

Chan C.Y., Beh S.L. and Broadhurst R.G., Homicide-suicide in Hong Kong, 1989-1998, Forensic Science International. 2003, 137(2-3): 165-171.

Chen Y.W., Liang A.C.T., Au W.Y., Chu K.M., Wong K.Y., Hu X., Lu L., Tang J.C.O., Chan K.W., Beh S.L., Kwong Y.L., Liang R.H.S. and Srivastava G., Multiple BCL6 translocation partners in individual cases of gastric lymphoma, Blood. 2003, 102: 1931-1932.

Lee K.W., Broadhurst R.G. and Beh S.L., Triad-related homicides in Hong Kong 1989-1998: a preliminary description, Crime and its Control in the People's Republic of China (Symposia Proceedings of the University of Hong Kong Centre for Criminology) Editor: R.G. Broadhurst. Hong Kong, Centre for Criminology, HKU, 2004, Part V, Chapter 23: 263-287.

Shen L., Liang A.C.T., Lu L., Au W.Y., Wong K.Y., Tin P.C., Chan K.W., Ko K.H., Chen W.Y.W., Beh S.L., Shimizu N., Tsuchiyama T., Tang J.C.O., Kwong Y.L., Liang R.H.S. and Srivastava G., Aberrant BCL10 nuclear expression in nasal NK/T-cell lymphoma, Blood. 2003, 102(4): 1553-1554.

Shen L., Liang A.C.T., Au W.Y., Lu L., Chen W.Y.W., Wong K.Y., Tang J.C.O., Chan K.W., Beh S.L., Kwong Y.L., Liang R.H.S. and Srivastava G., BCL10 mutations are irrelevant to its aberrant nuclear localization in nasal NK/T-cell lymphoma, Leukemia. 2003, 17(11): 2240-2242.

Wong T.W., Beh S.L., Lau C.C. and Tung W.K., A survey on the involvement of accident and emergency doctors in medicolegal work in Hong Kong, Journal of Clinical Forensic Medicine. 2004, 11(2): 75-77.

Researcher : Chan ASW

List of Research Outputs

Chan A.S.W., Tsui W.Y., Chen X., Chu K.M., Chan T.L., Chan A.S.Y., Li R., So S., Yuen S.T. and Leung S.Y., Down-regulation of ID4 by promoter hypermethylation in gastric adenocarcinoma, Proceedings of the International Conference on Applied Genomics, 9th European Society for Analytical Cellular Pathology meeting and 16th International Society for Diagnostic Quantitative Pathology meeting, Netherlands, 2003.

Chan A.S.W., Tsui W.Y., Chen X., Chu K.M., Chan T.L., Chan A.S.Y., Li R., So S., Yuen S.T. and Leung S.Y., Downregulation of ID4 by promoter hypermethylation in gastric adenocarcinoma, Oncogene. 2003, 22(44): 6946-6953.

Chan A.S.W., Chu K.M., Yuen S.T. and Leung S.Y., High-throughput quantification of hMLH1 promoter methylation using PyrosequencingTM technology , Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Chan T.L., Wong C.W., Chan A.S.W., Guo D., Ho J.W.C., Yuen S.T. and Leung S.Y., BRAF and KRAS mutations in mismatch repair deficient colorectal cancer , Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Researcher : Chan ASY

List of Research Outputs

Chan A.S.W., Tsui W.Y., Chen X., Chu K.M., Chan T.L., Chan A.S.Y., Li R., So S., Yuen S.T. and Leung S.Y., Down-regulation of ID4 by promoter hypermethylation in gastric adenocarcinoma, Proceedings of the International Conference on Applied Genomics, 9th European Society for Analytical Cellular Pathology meeting and 16th International Society for Diagnostic Quantitative Pathology meeting, Netherlands, 2003.

Chan A.S.W., Tsui W.Y., Chen X., Chu K.M., Chan T.L., Chan A.S.Y., Li R., So S., Yuen S.T. and Leung S.Y., Downregulation of ID4 by promoter hypermethylation in gastric adenocarcinoma, Oncogene. 2003, 22(44): 6946-6953.

Chan T.L., Chan Y.W., Ho J.W.C., Chan C., Chan A.S.Y., Chan E., Lam P.W.Y., Tse C.W., Lee K.C., Lau C.W., Gwi E., Leung S.Y. and Yuen S.T., MSH2 c.1452-1455delAATG is a founder mutation and an important cause of hereditary nonpolyposis colorectal cancer in the southern Chinese population, The American Journal of Human Genetics. 2004, 74(5): 1035-1042.

Chen X., Leung S.Y., Yuen S.T., Chu K.M., Ji J., Li R., Chan A.S.Y., Law S.Y.K., Troyanskaya O.G., Wong J., So S., Botstein D. and Brown P.O., Variation in gene expression patterns in human gastric cancers, Molecular Biology of the Cell. 2003, 14(8): 3208-3215.

Guo D., Yuen S.T., Ho J.W.C., Chan A.S.Y., Chen X. and Leung S.Y., PLA2G2A expression and its clinical significance in colorectal tumors, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Zhao W., Chan T.L., Chu K.M., Chan A.S.Y., Stratton M.R., Yuen S.T. and Leung S.Y., Mutations of BRAF and KRAS in gastric cancer and their association with microsatellite instability, International Journal of Cancer. 2004, 108(1): 167-169.

Researcher : Chan CF

List of Research Outputs

Chan C.F., Yau T.O., Jin D., Wong C.M., Fan S.T. and Ng I.O.L., Evaluation of nuclear factor-kB, urokinase-type plasminogen activator, and HBx and their clinicopathological significance in hepatocellular carcinoma, Clinical Cancer Research. 2004, 10(12 pt. 1): 4140-4149.

Chan C.F., M.Phil. Thesis, Dysregulation of NF-kB signaling in hepatocellular carcinoma. 2003.

Researcher : Chan DW

List of Research Outputs

Chan D.W., Lee M.F. and Ng I.O.L., Characterization of H-cadherin expression in human hepatocellular carcinoma, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Chan D.W. and Ng I.O.L., HBV X gene is required for maintaining the tumorigenicity of hepatocellular carcinoma cells, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Researcher : Chan EYT

List of Research Outputs

Li F.K., Tse K.C., Lam M.F., Yip T.P.S., Lui S.L., Chan G.S.W., Chan K.W., Chan E.Y.T., Choy C.B.Y., Lo W.K., Chan D.T.M. and Lai K.N., Incidence and outcome of antiglomerular basement membrane disease in Chinese, Nephrology (Carlton). 2004, 9: 100-104.

Mok T.M.Y., Chan E.Y.T., Leung K., Lo Y., Wong R.W.S. and Lau W.C.S., Antiphospholipid antibody profiles in Chinese patients with systemic lupus erythematosus, The Hong Kong Practitioner. 2004, 2: RI-04.

Researcher : Chan GSW

List of Research Outputs

Chan G.S.W., Lam M.F., Au W.Y. and Chan K.W., Pathologic quiz case: renal impairment after bone marrow transplantation. Bone marrow transplant nephropathy, Archives of Pathology and Laboratory Medicine. 2004, 128(2): 233–234.

Chan G.S.W., Yuen S.T. and Chan K.W., Synovial sarcoma presenting as a polypoid jejunal mass, Histopathology. 2004, 44(2): 191-193.

Li F.K., Tse K.C., Lam M.F., Yip T.P.S., Lui S.L., Chan G.S.W., Chan K.W., Chan E.Y.T., Choy C.B.Y., Lo W.K., Chan D.T.M. and Lai K.N., Incidence and outcome of antiglomerular basement membrane disease in Chinese, Nephrology (Carlton). 2004, 9: 100-104.

Researcher : Chan KW

Project Title:	Expression and somatic mutations of Fas (death receptor) gene in esophageal cancer
Investigator(s):	Dr. Chan K.W.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2002

Abstract:

To determine the expression of Fas and Fas-L in ESCC by immunohistochemistry; to examine the entire coding sequence of Fas mRNA from ESCC specimens and ESCC cell lines, sequence all splicing sites of this gene; to determine the chromosomal gene structure of this gene in these ESCCs.

Project Title:	Proteomic analysis of nasal NK-cell lymphoma: understanding the oncogenic process and in search of novel tumor markers
Investigator(s):	Dr. Chan K.W.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2003

Abstract:

To understand the proteomic aberrations involved in the malignant transformation of normal NK cell to NL, since alterations in protein expression underlie neoplastic behavior, this proteomic analysis will provide insights into the molecular pathogenesis of NL; to identify tumor markers of NL and develop assessment methods of these tumor markers.

List of Research Outputs

Chan G.S.W., Lam M.F., Au W.Y. and Chan K.W., Pathologic quiz case: renal impairment after bone marrow transplantation. Bone marrow transplant nephropathy, Archives of Pathology and Laboratory Medicine. 2004, 128(2): 233–234.

Chan G.S.W., Yuen S.T. and Chan K.W., Synovial sarcoma presenting as a polypoid jejunal mass, Histopathology. 2004, 44(2): 191-193.

Chan K.L., Ng S.P., Chan K.W., Wo Y.H. and Tam P.K.H., Pathogenesis of neonatal necrotizing enterocolitis: a study of the role of intraluminal pressure, age and bacterial concentration, Pediatric Surgery International. 2003, 19(8): 573-577.

Chen Y.W., Liang A.C.T., Au W.Y., Chu K.M., Wong K.Y., Hu X., Lu L., Tang J.C.O., Chan K.W., Beh S.L., Kwong Y.L., Liang R.H.S. and Srivastava G., Multiple BCL6 translocation partners in individual cases of gastric lymphoma, Blood. 2003, 102: 1931-1932.

Chim J.C.S., Li F.K. and Chan K.W., Cast nephropathy in multiple myeloma, Haematologica. 2004, 89(4): EIM08.

Guan Y., Zheng B., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S., Li P.H., Zhang L., Guan Y., Butt K.M., Wong K.L., Chan K.W., Lim W.W.L., Shortridge K.F., Yuen K.Y., Peiris J.S.M. and Poon L.L.M., Isolation and characterization of viruses related to the SARS Coronavirus from Animals in Southern China., Science. 2003, 302(5643): 276-278.

Huang F., Ma L., Wu A.Y.Y., Tian L., Chan K.W., Chan A.K., Lam A.C., Lo C.K., Ko K.H., To K.W., Chik S., Kam S.K., Fong I., Li X.S., Ng M.H. and MacPherson G.G., Systemic autoimmune disease induced by dendritic cells that have captured dying cells, Proceedings of the Frontiers in Biomedical Research, Hong Kong. 12 December 2003.

Lam M.F., Tse K.C., Chan K.W. and Au W.Y., Acute renal tubular necrosis due to grass carp ingestion in a myeloma patient after allogeneic stem cell transplantation, Bone Marrow Transplantation. 2004, 33: 669-670.

Li F.K., Lai C.K., Poon W.T., Chan A.Y.W., Chan K.W., Tse K.C., Chan D.T.M. and Lai K.N., Aggravation of non-steroidal anti-inflammatory drug-induced hepatitis and acute renal failure by slimming drug containing anthraquinones, Nephrology Dialysis Transplantation. 2004, 19(7): 1916-1917.

Li F.K., Tse K.C., Lam M.F., Yip T.P.S., Lui S.L., Chan G.S.W., Chan K.W., Chan E.Y.T., Choy C.B.Y., Lo W.K., Chan D.T.M. and Lai K.N., Incidence and outcome of antiglomerular basement membrane disease in Chinese, Nephrology (Carlton). 2004, 9: 100-104.

Ma L., Chan A.K., Lam A.C., Chan K.W., Lo C.K., Ko K.H., To K.W., Wu A.Y.Y., Tian L., Ng M.H. and Huang F., The role of dendritic cells in the induction of systemic autoimmune disease, Proceedings of the 8th Research Postgraduate Symposium, Hong Kong, 13 December 2003.

Shen L., Liang A.C.T., Lu L., Au W.Y., Wong K.Y., Tin P.C., Chan K.W., Ko K.H., Chen W.Y.W., Beh S.L., Shimizu N., Tsuchiyama T., Tang J.C.O., Kwong Y.L., Liang R.H.S. and Srivastava G., Aberrant BCL10 nuclear expression in nasal NK/T-cell lymphoma, Blood. 2003, 102(4): 1553-1554.

Shen L., Liang A.C.T., Au W.Y., Lu L., Chen W.Y.W., Wong K.Y., Tang J.C.O., Chan K.W., Beh S.L., Kwong Y.L., Liang R.H.S. and Srivastava G., BCL10 mutations are irrelevant to its aberrant nuclear localization in nasal NK/T-cell lymphoma, Leukemia. 2003, 17(11): 2240-2242.

Tse K.C., Chan D.T.M., Wong S.S.Y., Chan K.W., Lam M.F., Chan K.H.N., Li F.K., Chung B.C.Y. and Lai K.N., Granulomatous interstitial nephritis of the allograft kidney associated with rhodococcal pulmonary infection. Nephrol Dial Transplant, Nephrology Dialysis Transplantation. 2004, 19: 486-490.

Zheng B., Guan Y., Tang Q., Cheng D., Xie F.Y., He M.L., Chan K.W., Wong K.L., Lader E., Woodle M.C., Lu P.Y., Li B. and Zhong N., Prophylactic and therapeutic effects of small interfering RNA targeting SARS-coronavirus, Antiviral Therapy. 2004, 9: 365-374.

Researcher : Chan KYQ

List of Research Outputs

Chan K.Y.Q. and Chan K.Y.Q., Molecular study of GSPT1 in endometrial carcinoma. 2003.

Researcher : Chan LC

Project Title:	Mechanism of leukaemic transformation by fusion genes
Investigator(s):	Prof. Chan L.C., Dr. So C.W.
Department:	Pathology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	09/1999

Abstract:

To understand the molecular basis of leukaemia by studying the effects of a fusion gene in leukaemia on cellular transformation and identifying interactions with candidate proteins.

Project Title:	Mouse models of human leukaemia
Investigator(s):	Prof. Chan L.C., Dr. Sham M.H.
Department:	Pathology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	09/2002

Abstract:

The project attempts to understand the role of mixed lineage leukaemia gene (MLL) and EEN in th epathogenesis of leukaemia. Researchers will create mutant mice bearing the MLL-EEN fusion gene by gene targeting in embryonic stem (ES) cells. A conditional knock-in approach will be used in which MLL-EEN fusion gene will be expressed only in cells of the myeloid lineage after birth.

Project Title:	Subcellular localization and functional analysis of EEN/SH3GL1, a protein involved in leukaemia
Investigator(s):	Prof. Chan L.C., Dr. Jin D.Y., Dr. Poon R.Y.C.
Department:	Pathology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	07/2003

Abstract:

To sublocalise EEN/SH3GL1 in haemopoietic cells and leukaemias; to determine the regulatory roles of EEN/SH3GL1 in the cell cycle through, (a) microinjection of anti-EEN/SH3GL1 antibody and (b) suppression of EEN/SH3GL1 expression by RNA interference; to study the interaction of EEN/SH3GL1 2 with cyclins and cdks; to investigate whether EEN/SH3GL 1 is a substrate for phosphorylation by cdc2 and if so, to determine the role of the phosphorylated form of EEN/SH3GL 1 during cell cycle.

List of Research Outputs

Ambekar C.S., Lee J.S.K., Cheung B.M.Y., Chan L.C., Liang R.H.S. and Kumana C.R., Chloramphenicol succinate, a competitive substrate and inhibitor of succinate dehdrogenase : possible reason for its toxicity., Toxicology in Vitro. 2004, 18: 441-447.

Chan K.Y.K., Song Y., Mak W., Cheung A.N.Y., Peiris J.S.M., Yip S.P., Tin V., Liu W., Xue W.C., Chan L.C., Tam P.K.H., Ngan H.Y.S. and Khoo U.S., Polymorphisms of the ACE2 and CD13 (ANPEP) genes, and susceptibility to SARS coronavirus infection in Chinese from Hong Kong., The Human Genome organisation Meeting, April 2004, Berlin, Germany. 2004, Abstract No. 159.

Chan Y.K., Song Y.Q., Mak W., Cheung A.N.Y., Peiris J.S.M., Yip S.P., Tin V., Liu W., Xue W., Chan L.C., Tam P., Ngan H.Y.S. and Khoo U.S., Polymorphisms of the ACE2 and CD13 genes, and susceptibility to SARS coronavirus infection in Chinese from Hong Kong, Abstract for Human Genome Organization (HUGO) Meeting, April 4-7, 2004, Berlin, Germany. 2004.

Cheung N., So C.W. and Chan L.C., Functional characterization of EEN/endophilin 2, a fusion partner of MLL translocation, Proceedings of the FASEB Summer Research Conferences – Hematological Malignancies, Vermont, USA, 26-31 July 2003.

Chiu R.W.K., Ding C., Lau T.K., Leung T.N., Chan L.C., Chan A.Y.Y., Charoenkwan P., Ng I.S.L., Xu X.M., Wanapirak C., Sanguansermsri T., Liao C., Ma E.S.K., Chui D.H.K., Cantor C.R. and Lo Y.M.D., Non-invasive prenatal diagnosis of -thalassaemia by mass spectrometric analysis of fetal DNA in maternal plasma, Proceedings of the 9th International Conference on Thalassaemia and Haemoglobinopathies. Citt? del Mare, Terrasini, Palermo, Italy, 15–19 October 2003.

Khoo U.S., Chan Y.K., Yip S.P., Peiris M., Tin V., Liu W., Xue W., Ngan H.Y.S., Chan L.C. and Cheung A.N.Y., The tandem repeat polymorphism of the DC-SIGNR gene is associated with susceptibility to SARS coronavirus infection in Chinese from Hong Kong, Proceedings of the Human Genome Organization (HUGO) Meeting, April 4-7, 2004, Berlin, Germany. 2004.

Kong C.T., Chan L.C. and Sham M.H., Mll-Een affects early lineage commitment of embryonic stem cells and cause maturation arrest of myeloid progenitors, FASEBSummer Research Conference on Hematologic Malignancies 26-31 July 2003, Vermount, USA. 2003.

Lee C.K., Ma E.S.K., Shek T.W.H., Lam C.C.K., Au W.Y., Wan T.S.K. and Chan L.C., Plasmablastic transformation of multiple myeloma, Human Pathology. 2003, 34(7): 710-714.

Lee C.K., Ma E.S.K., Tang M., Lam C.C.K., Lin C.K. and Chan L.C., Prevalence and specificity of clinically significant red cell alloantibodies in Chinese women during pregnancy - a review of cases from 1997 to 2001, Transfusion Medicine. 2003, 13(4): 227.

Leung K.F.S., Ma E.S.K., Chan A.Y.Y. and Chan L.C., Clinical phenotype of haemoglobin Q-H disease, Journal of Clinical Pathology. 2004, 57: 81-82.

Ma E.S.K., Kwok J.S.Y., Li Y.H. and Chan L.C., Clinical significance of ADAMTS13 mutational analysis in relapsing TTP with severe vWF-cleaving protease deficiency, Blood. 2003, 102(11) Part 1: 802a.

Ma E.S.K., Kwok J.S.Y., Li Y.H. and Chan L.C., Clinical significance of ADAMTS13 mutational analysis in relapsing TTP with severe vWF-cleaving protease deficiency, Proceedings of the American Society of Hematology, San Diego, USA, 6-9 December 2003. Abstract No. 2970.

Wan T.S.K., Ma E.S.K., Li C.K. and Chan L.C., Atypical fluorescence in situ hybridisation pattern in chronic myeloid leukaemia due to cryptic insertion of BCR at 9q34, Leukemia. 2004, 18(1): 161-162.

Wan T.S.K., Ma E.S.K., Lam C.C.K., Chan L.C., Lee K.K. and Au W.Y., Deletion 9q as the sole karyotypic abnormality in myelocytic disorders: a new case of myelodysplastic syndrome and its prognostic implications in acute myelocytic leukemia, Cancer Genetics and Cytogenetics. 2003, 145(2): 184-186.

Wang W., Tan A.S.-.C., Kham S.K.Y., Chan A.Y.Y., Prior J., Erber W.N., Ma E.S.K., Chan L.C., Chui D.H.K., Quah T.C. and Chong S.S., Improved tools for molecular diagnosis of a- and b-thalassemia in Southeast Asia, Proceedings of the 9th International Conference on Thalassaemia and Haemoglobinopathies. Citt? del Mare, Terrasini, Palermo, Italy, 15-19 October 2003.

Wang W., Ma E.S.K., Chan A.Y.Y., Prior J., Erber W.N., Chan L.C., Chui D.H.K. and Chong S.S., Single-tube multiplex-PCR screen for anti-3.7 and anti-4.2 a-globin gene triplications, Clinical Chemistry. 2003, 49(10): 1679-1682.

Yam J.W.P., Jin D., So C.W. and Chan L.C., Identification and characterization of EBP, a novel EEN binding protein that inhibits Ras signaling and is recruited into the nucleus by the MLL-EEN fusion protein, Blood. 2004, 103(4): 1445-1453.

Yam J.W.P. and Chan L.C., MLL-EEN fusion protein recruits the cytoplasmic EEN binding protein, EBP into the nucleus, Proceedings of the FASEB Summer Research Conferences – Hematological Malignancies, Vermont, USA, 26-31 July 2003.

Researcher : Chan QKY

List of Research Outputs

Yang C., Ngan H.Y.S., Khoo U.S., Chan Y.K., Chiu P.M., Chan Q.K.Y., Xue W. and Cheung A.N.Y., Single nucleotide polymorphisms (SNPS) in the coding sequence of follicle stimulating hormone receptor (FSHR) is associated with ovarian cancer susceptibility, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Researcher : Chan TL

List of Research Outputs

Chan A.S.W., Tsui W.Y., Chen X., Chu K.M., Chan T.L., Chan A.S.Y., Li R., So S., Yuen S.T. and Leung S.Y., Down-regulation of ID4 by promoter hypermethylation in gastric adenocarcinoma, Proceedings of the International Conference on Applied Genomics, 9th European Society for Analytical Cellular Pathology meeting and 16th International Society for Diagnostic Quantitative Pathology meeting, Netherlands, 2003.

Chan A.S.W., Tsui W.Y., Chen X., Chu K.M., Chan T.L., Chan A.S.Y., Li R., So S., Yuen S.T. and Leung S.Y., Downregulation of ID4 by promoter hypermethylation in gastric adenocarcinoma, Oncogene. 2003, 22(44): 6946-6953.

Chan T.L., Zhao W., Cancer Genome Project .., Leung S.Y. and Yuen S.T., BRAF and KRAS mutations in colorectal hyperplastic polyps and serrated adenomas, Cancer Research. 2003, 63: 4878-4881.

Chan T.L., Wong C.W., Chan A.S.W., Guo D., Ho J.W.C., Yuen S.T. and Leung S.Y., BRAF and KRAS mutations in mismatch repair deficient colorectal cancer , Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Chan T.L., Chan Y.W., Ho J.W.C., Chan C., Chan A.S.Y., Chan E., Lam P.W.Y., Tse C.W., Lee K.C., Lau C.W., Gwi E., Leung S.Y. and Yuen S.T., MSH2 c.1452-1455delAATG is a founder mutation and an important cause of hereditary nonpolyposis colorectal cancer in the southern Chinese population, The American Journal of Human Genetics. 2004, 74(5): 1035-1042.

Zhao W., Chan T.L., Chu K.M., Chan A.S.Y., Stratton M.R., Yuen S.T. and Leung S.Y., Mutations of BRAF and KRAS in gastric cancer and their association with microsatellite instability, International Journal of Cancer. 2004, 108(1): 167-169.

Researcher : Chan YK (Chan Yiu Kwan)

List of Research Outputs

Xue W., Khoo U.S., Ngan H.Y.S., Chan Y.K., Chiu P.M., Tsao G.S.W. and Cheung A.N.Y., Minichromosome maintenance protein 7 expression in gestational trophoblastic disease: correlation with Ki67, PCNA and clinicopathological parameters, Histopathology. 2003, 43: 485-490.

Researcher : Chan YK (Chan Yuen Kwong)

List of Research Outputs

Chan Y.K., Shen D.H., Li S.S., Khoo U.S., Ngan H.Y.S., Xue W. and Cheung A.N.Y., Differential Expression and Methylation of p33/ING1b in Ovarian Tumors, Proceedings of the 94th Annual Meeting of the American Association for Cancer Research, Washington, USA, 11-14 July 2003.

Chan Y.K., Song Y.Q., Mak W., Cheung A.N.Y., Peiris J.S.M., Yip S.P., Tin V., Liu W., Xue W., Chan L.C., Tam P., Ngan H.Y.S. and Khoo U.S., Polymorphisms of the ACE2 and CD13 genes, and susceptibility to SARS coronavirus infection in Chinese from Hong Kong, Abstract for Human Genome Organization (HUGO) Meeting, April 4-7, 2004, Berlin, Germany. 2004.

Chan Y.K., Khoo U.S., Chan K.H.K., Ngan H.Y.S., Li S.S., Xue W., Chiu P.M. and Cheung A.N.Y., Reduced GSTP1 immunoreactivity in endometrial carcinomas in association with methylation status and genetic profiles, Proceedings of the 94th Annual Meeting of the American Association for Cancer Research, Washington, USA, 11-14 July 2003.

Cheung A.N.Y., Szeto E.F., Ng K.M., Fong K.W., Ang Chan E.Y., Tsun O.K.L., Khoo U.S., Chan Y.K. and Ng W.Y., Atypical squamous cells of undetermined significance on cervical smears: follow-up study of an Asian screening population, Cancer Cytopathology. 2004, 102(2): 74-80.

Cheung A.N.Y., Khoo U.S., Lai C.Y.L., Chan Y.K., Xue W., Cheng D.K.L., Chiu P.M., Tsao G.S.W. and Ngan H.Y.S., Metastatic trophoblastic disease after an initial diagnosis of partial hydatidiform mole: genotyping and chromosome in situ hybridization analysis, Cancer. 2004, 100(7): 1411-1417.

Khoo U.S., Koo W.T., Chan Y.K. and Cheung A.N.Y., DNA-based detection of breast cancer in fluid arising from Fine-Needle-Aspiration Cytology samples, Proceedings of the 94th Annual Meeting of the American Association for Cancer Research, Washington, USA, 11-14 July 2003.

Khoo U.S., Chan Y.K., Yip S.P., Peiris M., Tin V., Liu W., Xue W., Ngan H.Y.S., Chan L.C. and Cheung A.N.Y., The tandem repeat polymorphism of the DC-SIGNR gene is associated with susceptibility to SARS coronavirus infection in Chinese from Hong Kong, Proceedings of the Human Genome Organization (HUGO) Meeting, April 4-7, 2004, Berlin, Germany. 2004.

Lai C.Y.L., Chan Y.K., Khoo U.S., Ngan H.Y.S., Xue W., Chiu P.M., Tsao G.S.W. and Cheung A.N.Y., Analysis of gestational trophoblastic disease by genotyping and chromosome in situ hybridization, Modern Pathology. 2004, 17: 40-48.

Liu S., Leung C.Y., Chan Y.K., Chiu P.M., Cheung A.N.Y., Tam K.F., Ng T.Y., Wong R.L.C. and Ngan H.Y.S., p73 expression is associated with the cellular radiosensitivity in cervical cancer after radiotherapy, Clinical Cancer Research. 2004, 10: 3309-3316.

Xue W., Feng H., Tsao G.S.W., Chan Y.K., Ngan H.Y.S., Chiu P.M., Maccalman C.D. and Cheung A.N.Y., Methylation status and expression of E-cadherin and cadherin-11 in gestational trophoblastic diseases, International Journal of Gynecological Cancer. 2003, 13(6): 879-888.

Xue W., Khoo U.S., Ngan H.Y.S., Chan Y.K., Ip P.P.C., Tsao G.S.W. and Cheung A.N.Y., c-mos immunoreactivity aids in the diagnosis of gestational trophoblastic lesions, International Journal of Gynecological Pathology. 2004, 23(2): 145-150.

Yang C., Ngan H.Y.S., Khoo U.S., Chan Y.K., Chiu P.M., Chan Q.K.Y., Xue W. and Cheung A.N.Y., Single nucleotide polymorphisms (SNPS) in the coding sequence of follicle stimulating hormone receptor (FSHR) is associated with ovarian cancer susceptibility, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Yang H., Liu V.W.S., Wang Y., Chan Y.K., Tsang P.C.K., Khoo U.S., Cheung A.N.Y. and Ngan H.Y.S., Detection of hypermethylated genes in tumor and plasma of cervical cancer patients, Gynecologic Oncology. 2004, 93(2): 435-440.

Researcher : Chan YW

List of Research Outputs

Chan T.L., Chan Y.W., Ho J.W.C., Chan C., Chan A.S.Y., Chan E., Lam P.W.Y., Tse C.W., Lee K.C., Lau C.W., Gwi E., Leung S.Y. and Yuen S.T., MSH2 c.1452-1455delAATG is a founder mutation and an important cause of hereditary nonpolyposis colorectal cancer in the southern Chinese population, The American Journal of Human Genetics. 2004, 74(5): 1035-1042.

Researcher : Chen WYW

List of Research Outputs

Law B.F.F., Srivastava G., Wong K.Y., Chen W.Y.W., Liang A.C.T., Lam K.Y., Law S.Y.K., Wong J. and Tang J.C.O., A novel tumor transforming gene at 7q22, GAESCC1, is frequently amplified and overexpressed in primary esophageal squamous cell carcinomas, Abstract of the Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Shen L., Liang A.C.T., Lu L., Au W.Y., Wong K.Y., Tin P.C., Chan K.W., Ko K.H., Chen W.Y.W., Beh S.L., Shimizu N., Tsuchiyama T., Tang J.C.O., Kwong Y.L., Liang R.H.S. and Srivastava G., Aberrant BCL10 nuclear expression in nasal NK/T-cell lymphoma, Blood. 2003, 102(4): 1553-1554.

Shen L., Liang A.C.T., Au W.Y., Lu L., Chen W.Y.W., Wong K.Y., Tang J.C.O., Chan K.W., Beh S.L., Kwong Y.L., Liang R.H.S. and Srivastava G., BCL10 mutations are irrelevant to its aberrant nuclear localization in nasal NK/T-cell lymphoma, Leukemia. 2003, 17(11): 2240-2242.

Researcher : Chen YW

List of Research Outputs

Chen Y.W., Molecular Genetics of Gastric Non Hodgkins B-cell Lymphomas. 2004.

Chen Y.W., Liang A.C.T., Au W.Y., Chu K.M., Wong K.Y., Hu X., Lu L., Tang J.C.O., Chan K.W., Beh S.L., Kwong Y.L., Liang R.H.S. and Srivastava G., Multiple BCL6 translocation partners in individual cases of gastric lymphoma, Blood. 2003, 102: 1931-1932.

Researcher : Cheung ANY

Project Title:	Differential expression of genes in gestational trophoblastic diseases
Investigator(s):	Dr. Cheung A.N.Y., Prof. Ngan H.Y.S., Prof. Tsao G.S.W.
Department:	Pathology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	10/2000

Abstract:

To conduct a comprehensive investigation on gene expression in gestational trophoblastic diseases (GTD) using the cDNA array hybridization and the PCR based suppression substractive hybridzation (SSH) methods.

Project Title:	Genetic alterations in mitochondrial DNA in gestational trophoblastic diseases (GTD)
Investigator(s):	Dr. Cheung A.N.Y., Dr. Liu V.W.S., Prof. Ngan H.Y.S.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2001
Completion Date:	10/2003

Abstract:

To identify somatic mutations and polymorphisms of mitochondrial DNA (mtDNA) in hydatidiform mole (HM) using extra-long and standard polymerase chain reactions and automated DNA sequencing.

Project Title:	Methylation and expression analysis of p33/ING1 tumor-suppressor gene in ovarian endometrial and ovarian cancer
Investigator(s):	Dr. Cheung A.N.Y., Prof. Ngan H.Y.S.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2002

Abstract:

To investigate whether the status of expression and methylation of p33/ING1 contributes significantly in hte pathogenesis and progression of endometrial and ovarian cancer.

Project Title:	DNA methylation and histone acetylation profile in gestational trophoblastic
Investigator(s):	Dr. Cheung A.N.Y., Dr. Chan K.Y.K., Dr. Khoo U.S., Prof. Ngan H.Y.S.
Department:	Pathology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	07/2003

Abstract:

To analyze the DNA methylation and histone acetylation status of p53, mdm2, p21^WAF, Mc1-1,bc1-2, p16, E-cadherin, caspases 8 and 10 in hydatidiform mole in an attempt to evaluate the role of such epigenetic changes in the pathogenesis of trophoblastic disease; to recognize genetic markers that may be able to predict the clinical progression of hydatidiform mole; to explore potential use of DNA methylation and histone acetylation inhibitors in future treatment.

Project Title:	Pi-class Glutathione-S-Transferase in endometrial carcinoma
Investigator(s):	Dr. Cheung A.N.Y., Dr. Khoo U.S., Prof. Ngan H.Y.S.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2003

Abstract:

To investigate whether the genetic alteration, status of expression and methylation of GSTP1 contributes significantly to the pathogenesis and progression of endometrial cancer.

List of Research Outputs

Ajonuma L.C., Ng E.H., Chow P.H., Cheung A.N.Y. and Chan H.C., Ultrastructural examination of hydrosalpinx from infertile patients presenting for infertility treatment, Proceedings of the New Challenges in Health Care, 10th Anniversary of Hong Kong Academy of Medicine, 28 November 2003. Proceedings 103: Poster 1.

Basir G.S., Cheung P.T., Cheung A.N.Y. and Lao T.T.H., Effect of the mode of conception on incidences of apoptotic and proliferative cells in full term human placentas, Abstract for 194th Meeting of the Society for Endocrinology and Society for Endocrinology jointly with Diabetes U.K., November 3-5, 2003, London, U.K. . 2003.

Basir G.S., Cheung P.T., Cheung A.N.Y. and Lao T.T.H., Immunohistochemical localization of insulin like growth factor 1 (IGF-1) and insulin like growth factor 1 receptors (IGF-1R) in the placentas of normal and complicated pregnancies, 20th Anniversary (1983-2003) and 15th Annual Scientific Meeting held by Hong Kong Society of Endocrinology, Metabolism and Reproduction, October 19, 2003, Hong Kong. 2003.

Chan C.C.W., Lao T.T.H., Ho P.C., Sung E.O.P. and Cheung A.N.Y., The effect of mifepristone on the expression of steroid hormone receptors in human decidua and placenta: a randomized placebo-controlled double-blind study, Journal of Clinical Endocrinology & Metabolism. 2003, 88(12): 5846-5850.

Chan K.Y.K., Song Y., Mak W., Cheung A.N.Y., Peiris J.S.M., Yip S.P., Tin V., Liu W., Xue W.C., Chan L.C., Tam P.K.H., Ngan H.Y.S. and Khoo U.S., Polymorphisms of the ACE2 and CD13 (ANPEP) genes, and susceptibility to SARS coronavirus infection in Chinese from Hong Kong., The Human Genome organisation Meeting, April 2004, Berlin, Germany. 2004, Abstract No. 159.

Chan Y.K., Shen D.H., Li S.S., Khoo U.S., Ngan H.Y.S., Xue W. and Cheung A.N.Y., Differential Expression and Methylation of p33/ING1b in Ovarian Tumors, Proceedings of the 94th Annual Meeting of the American Association for Cancer Research, Washington, USA, 11-14 July 2003.

Chan Y.K., Song Y.Q., Mak W., Cheung A.N.Y., Peiris J.S.M., Yip S.P., Tin V., Liu W., Xue W., Chan L.C., Tam P., Ngan H.Y.S. and Khoo U.S., Polymorphisms of the ACE2 and CD13 genes, and susceptibility to SARS coronavirus infection in Chinese from Hong Kong, Abstract for Human Genome Organization (HUGO) Meeting, April 4-7, 2004, Berlin, Germany. 2004.

Chan Y.K., Khoo U.S., Chan K.H.K., Ngan H.Y.S., Li S.S., Xue W., Chiu P.M. and Cheung A.N.Y., Reduced GSTP1 immunoreactivity in endometrial carcinomas in association with methylation status and genetic profiles, Proceedings of the 94th Annual Meeting of the American Association for Cancer Research, Washington, USA, 11-14 July 2003.

Cheung A.N.Y., Szeto E.F., Ng K.M., Fong K.W., Ang Chan E.Y., Tsun O.K.L., Khoo U.S., Chan Y.K. and Ng W.Y., Atypical squamous cells of undetermined significance on cervical smears: follow-up study of an Asian screening population, Cancer Cytopathology. 2004, 102(2): 74-80.

Cheung A.N.Y., Challenges faced by healthcare workers and Pathologists: Hong Kong perspective, Proceedings of the First Asian HPV, Sydney, Australia, 11-14 November 2003.

Cheung A.N.Y., Szeto E.F., Leung B.S.Y., Khoo U.S. and Ng W.Y., Liquid-based cytology and conventional cervical smears: a comparison study in an Asian screening population, Cancer. 2003, 99(6): 331-335.

Cheung A.N.Y., Khoo U.S., Lai C.Y.L., Chan Y.K., Xue W., Cheng D.K.L., Chiu P.M., Tsao G.S.W. and Ngan H.Y.S., Metastatic trophoblastic disease after an initial diagnosis of partial hydatidiform mole: genotyping and chromosome in situ hybridization analysis, Cancer. 2004, 100(7): 1411-1417.

Cheung A.N.Y., Molecular Oncology: ABC in Gynecologic oncology, Proceedings of the 10th Hong Kong International Cancer Congress, 19-21 November 2003.

Cheung A.N.Y., Pathology of gestational trophoblastic diseases, Best Practice & Research Clinical Obstetrics and Gynaecology. 2003, 17(6): 849-868.

Cheung A.N.Y., Xue W., Chan D.Y., Feng H., Chiu P.M., Tsao G.S.W. and Ngan H.Y.S., Promoter hypermethylation of multiple genes in hydatiform mole choriocarcinoma, Proceedings of the American Association for Cancer Research. 2004, 45: 1149 Abstract No. 4974.

Cheung A.N.Y., Khoo U.S., Xue W. and Shen D.H., Recent advances in the research of gestational trophoblastic diseases, 妊娠滋??胞病?的研究?展, Chinese Journal of Pathology. 中?病理??志 , 2003, 32(4): 373-375.

Cheung A.N.Y., Recent advances on Pathology, and Research on gestational Trophoblastic Disease, Proceedings of the 8th Annual Scientific Meeting, Pathology Division of Chinese Medical Association, Jinan, China, 3-6 April 2004. Proceedings 83-84.

Chiu P.M., Liu V.W.S., Ngan H.Y.S., Khoo U.S. and Cheung A.N.Y., Detection of mitochondrial DNA mutations in gestational trophoblastic disease, Human Mutation. 2003, 22(2): 177.

Feng H., Xue W., Cheung A.N.Y., Wang Y.L. and Tsao G.S.W., Differentially expressed genes between ormal placenta and choriocarcinoma, Proceedings of the American Society for Cell Biology, 43rd Annual Meeting. 2003, Abstract No. 2774.

Feng H., Cheung A.N.Y., Xue W., Wang Y., Wang X., Fu S., Wang Q., Ngan H.Y.S. and Tsao G.S.W., Down-regulation and promoter methylation of tissue inhibitor of metalloproteinase 3 in choriocarcinoma, Gynecologic Oncology. 2004, 94: 375-382.

Fu M., Lui V.C.H., Sham M.H., Cheung A.N.Y. and Tam P.K.H., HOXB5 expression is spatially and temporarily regulated in human embryonic gut during neural crest cell colonization and differentiation of enteric neuroblasts, Developmental Dynamics. 2003, 228(1): 1-10.

Khoo U.S., Koo W.T., Chan Y.K. and Cheung A.N.Y., DNA-based detection of breast cancer in fluid arising from Fine-Needle-Aspiration Cytology samples, Proceedings of the 94th Annual Meeting of the American Association for Cancer Research, Washington, USA, 11-14 July 2003.

Khoo U.S., Chan Y.K., Yip S.P., Peiris M., Tin V., Liu W., Xue W., Ngan H.Y.S., Chan L.C. and Cheung A.N.Y., The tandem repeat polymorphism of the DC-SIGNR gene is associated with susceptibility to SARS coronavirus infection in Chinese from Hong Kong, Proceedings of the Human Genome Organization (HUGO) Meeting, April 4-7, 2004, Berlin, Germany. 2004.

Lai C.Y.L., Chan Y.K., Khoo U.S., Ngan H.Y.S., Xue W., Chiu P.M., Tsao G.S.W. and Cheung A.N.Y., Analysis of gestational trophoblastic disease by genotyping and chromosome in situ hybridization, Modern Pathology. 2004, 17: 40-48.

Leung T.W., Xue W., Cheung A.N.Y., Khoo U.S. and Ngan H.Y.S., Proliferation to apoptosis ratio as a prognostic marker in adenocarcinoma of uterine cervix, Gynecologic Oncology. 2004, 92(3): 866-872.

Li H.W.R., Yu M.M.Y., Cheung A.N.Y. and Wong Y.F., Expression of leptin receptors in gestational trophoblastic diseases, Proceedings of the 11th World Congress of Gynecological Endocrinology, Florence, Italy, 26-29 February 2004.

Liu S., Cheung A.N.Y. and Ngan H.Y.S., p73 Expression and radiosensitivity in cervical carcinomas, American Association for Cancer Research 94th Annual Meeting, July 11-14, 2003, Toronto, Canada. 2003.

Liu S., Leung C.Y., Chan Y.K., Chiu P.M., Cheung A.N.Y., Tam K.F., Ng T.Y., Wong R.L.C. and Ngan H.Y.S., p73 expression is associated with the cellular radiosensitivity in cervical cancer after radiotherapy, Clinical Cancer Research. 2004, 10: 3309-3316.

Liu V.W.S., Yang H., Wang Y., Tsang P.C.K., Cheung A.N.Y., Chiu P.M., Ng T.Y., Wong R.L.C., Nagley P. and Ngan H.Y.S., High frequency of mitochondrial genome instability in human endometrial carcinomas, British Journal of Cancer. 2003, 89(4): 697-701.

Shen D., Khoo U.S., Ngan H.Y.S., Ng T.Y., Chau M.T., Xue W. and Cheung A.N.Y., Coexisting epithelioid trophoblastic tumor and choriocarcinoma of the uterus following a chemoresistant hydatidiform mole, Archives of Pathology and Laboratory Medicine. 2003, 127(7): e291–e293.

Tam K.F., Cheung A.N.Y., Liu K.L., Ng T.Y., Pun T.C., Chan Y.M., Wong R.L.C., Ng W.Y. and Ngan H.Y.S., A retrospective review on atypical glandular cells of undetermined significance (AGUS) using the Bethesda 2001 classification, Gynecologic Oncology. 2003, 91(3): 603-607.

Tse J.Y.M., Wong E.Y.M., Cheung A.N.Y., O W.S., Tam P.C. and Yeung W.S.B., Specific expression of VCY2 in human male germ cells and its involvement in the pathogenesis of male infertility , Biology of Reproduction. 2003, 69: 746-751.

Xue W., Feng H., Tsao G.S.W., Chan Y.K., Ngan H.Y.S., Chiu P.M., Maccalman C.D. and Cheung A.N.Y., Methylation status and expression of E-cadherin and cadherin-11 in gestational trophoblastic diseases, International Journal of Gynecological Cancer. 2003, 13(6): 879-888.

Xue W., Khoo U.S., Ngan H.Y.S., Chan Y.K., Chiu P.M., Tsao G.S.W. and Cheung A.N.Y., Minichromosome maintenance protein 7 expression in gestational trophoblastic disease: correlation with Ki67, PCNA and clinicopathological parameters, Histopathology. 2003, 43: 485-490.

Xue W., Khoo U.S., Ngan H.Y.S., Chan Y.K., Ip P.P.C., Tsao G.S.W. and Cheung A.N.Y., c-mos immunoreactivity aids in the diagnosis of gestational trophoblastic lesions, International Journal of Gynecological Pathology. 2004, 23(2): 145-150.

Yang C., Ngan H.Y.S., Khoo U.S., Chan Y.K., Chiu P.M., Chan Q.K.Y., Xue W. and Cheung A.N.Y., Single nucleotide polymorphisms (SNPS) in the coding sequence of follicle stimulating hormone receptor (FSHR) is associated with ovarian cancer susceptibility, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Yang H., Liu V.W.S., Tsang P.C.K., Yip M.W., Ng T.Y., Cheung A.N.Y. and Ngan H.Y.S., Comparison of human papillomavirus DNA levels in gynecological cancers: implication for cancer development, Tumour Biology. 2003, 24(6): 310-316.

Yang H., Liu V.W.S., Wang Y., Chan Y.K., Tsang P.C.K., Khoo U.S., Cheung A.N.Y. and Ngan H.Y.S., Detection of hypermethylated genes in tumor and plasma of cervical cancer patients, Gynecologic Oncology. 2004, 93(2): 435-440.

Researcher : Cheung N

List of Research Outputs

Cheung N., So C.W. and Chan L.C., Functional characterization of EEN/endophilin 2, a fusion partner of MLL translocation, Proceedings of the FASEB Summer Research Conferences – Hematological Malignancies, Vermont, USA, 26-31 July 2003.

Researcher : Ching YP

Project Title:	The functional characterisation of Pak5 and caspase 4 interaction- a role in apoptosis
Investigator(s):	Dr. Ching Y.P.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	02/2004

Abstract:

To confirm the interaction of Pak5 and caspase 4 using three independent assays, including co-immunoprecipitation, co-immunostaining and GST affinity pull down assays; to map the minimal binding region of these two proteins and develop functional assays to assess the functional outcome of this interaction; to determine if Pak5 plays a role in apoptosis by regulating the caspase 4.

List of Research Outputs

Ching Y.P., Leong V.Y.L., Wong C.M. and Kung H., Identification of an autoinhibitory domain of p21-activated protein kinase 5, Journal of Biological Chemistry. 2003, 278(36): 33621-33624.

Ching Y.P., Leong V.Y.L., Jin D. and Ng I.O.L., Regulation of the tumour metastasis suppressor gene, NM23, by p21-activated protein kinase 5., Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Leung H.Y., Ching Y.P., Wong C.M., Ng D.C.H., Jin D. and Ng I.O.L., Characterization of novel putative tumor suppressor gene, DLC2 (deleted in liver cancer 2), in hepatocellular carcinoma, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Qi R.Z., Ching Y.P., Kung H. and Wang J.H., a-Chimaerin exists in a functional complex with the Cdk5 kinase in brain, FEBS Letters. 2004, 561(1-3): 177-180.

Sze M.F., Ching Y.P., Jin D. and Ng I.O.L., Dysregulation of mitotic spindle checkpoint control in hepatocellular carcinomas, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Wong C.M., Ching Y.P., Lee M.F., Jin D. and Ng I.O.L., Genetic and epigenetic alterations of DLC-1 gene in hepatocellular carcinoma, Cancer Research. 2003, 63: 7646-7651.

Yang Y., Lin M.C., Ching Y.P., Lam S.K., Xia H.H.X., Tu S., Zou B., Wang J., Li G., He H., Gu Q., Peng J., Kung H. and Wong B.C.Y., FHL2 As a Co-Factor of XAF1 in the Induction of Apoptosis, Gastroenterology. 2004, 126 (4 Suppl 2): S938.

Researcher : Chiu PM

List of Research Outputs

Chan Y.K., Khoo U.S., Chan K.H.K., Ngan H.Y.S., Li S.S., Xue W., Chiu P.M. and Cheung A.N.Y., Reduced GSTP1 immunoreactivity in endometrial carcinomas in association with methylation status and genetic profiles, Proceedings of the 94th Annual Meeting of the American Association for Cancer Research, Washington, USA, 11-14 July 2003.

Cheung A.N.Y., Khoo U.S., Lai C.Y.L., Chan Y.K., Xue W., Cheng D.K.L., Chiu P.M., Tsao G.S.W. and Ngan H.Y.S., Metastatic trophoblastic disease after an initial diagnosis of partial hydatidiform mole: genotyping and chromosome in situ hybridization analysis, Cancer. 2004, 100(7): 1411-1417.

Cheung A.N.Y., Xue W., Chan D.Y., Feng H., Chiu P.M., Tsao G.S.W. and Ngan H.Y.S., Promoter hypermethylation of multiple genes in hydatiform mole choriocarcinoma, Proceedings of the American Association for Cancer Research. 2004, 45: 1149 Abstract No. 4974.

Chiu P.M., Liu V.W.S., Ngan H.Y.S., Khoo U.S. and Cheung A.N.Y., Detection of mitochondrial DNA mutations in gestational trophoblastic disease, Human Mutation. 2003, 22(2): 177.

Lai C.Y.L., Chan Y.K., Khoo U.S., Ngan H.Y.S., Xue W., Chiu P.M., Tsao G.S.W. and Cheung A.N.Y., Analysis of gestational trophoblastic disease by genotyping and chromosome in situ hybridization, Modern Pathology. 2004, 17: 40-48.

Liu S., Leung C.Y., Chan Y.K., Chiu P.M., Cheung A.N.Y., Tam K.F., Ng T.Y., Wong R.L.C. and Ngan H.Y.S., p73 expression is associated with the cellular radiosensitivity in cervical cancer after radiotherapy, Clinical Cancer Research. 2004, 10: 3309-3316.

Liu V.W.S., Yang H., Wang Y., Tsang P.C.K., Cheung A.N.Y., Chiu P.M., Ng T.Y., Wong R.L.C., Nagley P. and Ngan H.Y.S., High frequency of mitochondrial genome instability in human endometrial carcinomas, British Journal of Cancer. 2003, 89(4): 697-701.

Xue W., Feng H., Tsao G.S.W., Chan Y.K., Ngan H.Y.S., Chiu P.M., Maccalman C.D. and Cheung A.N.Y., Methylation status and expression of E-cadherin and cadherin-11 in gestational trophoblastic diseases, International Journal of Gynecological Cancer. 2003, 13(6): 879-888.

Xue W., Khoo U.S., Ngan H.Y.S., Chan Y.K., Chiu P.M., Tsao G.S.W. and Cheung A.N.Y., Minichromosome maintenance protein 7 expression in gestational trophoblastic disease: correlation with Ki67, PCNA and clinicopathological parameters, Histopathology. 2003, 43: 485-490.

Yang C., Ngan H.Y.S., Khoo U.S., Chan Y.K., Chiu P.M., Chan Q.K.Y., Xue W. and Cheung A.N.Y., Single nucleotide polymorphisms (SNPS) in the coding sequence of follicle stimulating hormone receptor (FSHR) is associated with ovarian cancer susceptibility, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Researcher : Choy CKW

List of Research Outputs

Au W.Y., Gascoyne R.D., Gallaghar R.E., Nhu L., Klasa R.D., Liang R.H.S., Choy C.K.W., Foo W. and Connors J.M., Hodgkin's disease in Chinese migrants to British Columbia: a 25-year survey, Annals of Oncology. 2004, 15: 626-630.

Au W.Y., Chan C.P., Chung L.P. and Choy C.K.W., Primary B-Cell lymphoma and lymphoma-like lesions of the uterine cervix, American Journal of Hematology. 2003, 73: 176-179.

Au W.Y., Pang A.W.K., Choy C.K.W., Chim J.C.S. and Kwong Y.L., Quantification of circulating Epstein-Barr virus (EBV) DNA in the diagnosis and monitoring of natural killer cell and EBV-positive lymphomas in immunocompetent patients, Blood. 2004, 104(1): 243-9.

Au W.Y., Lie A.K.W., Chan C., Pang A.W.K., Liang R.H.S., Ma E.S.K., Choy C.K.W. and Kwong Y.L., Treatment of post-renal transplantation lymphoproliferative disease manifesting as plasmacytoma with non-myeloablative hematopoietic stem cell transplantation from the same kidney donor, American Journal of Hematology. 2003, 74: 283-6.

Chim J.C.S., Ma A.Y., Au W.Y., Choy C.K.W., Lie A.K.W., Liang R.H.S., Yau C.C. and Kwong Y.L., Primary nasal natural killer cell lymphoma : long term treatment outcome and relationship with the international prognostic index., Blood. 2004, 103: 216-221.

Hon C., Au W.Y., Chung L.P. and Choy C.K.W., Allogeneic stem cell transplantation for therapy related leukemia after bilateral retinoblastoma in a girl with del(13q) syndrome, Annals of Hematology. 2004, 75: 115-6.

Researcher : Chung LP

Project Title:	Expression of angiopoietins and their receptor (Tie2) in tumours
Investigator(s):	Dr. Chung L.P., Dr. Leung S.Y., Dr. Wong M.P., Dr. Yuen S.T.
Department:	Pathology (Hospital Pathology Services Morbid Anatomy/Cytology)
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	07/1998

Abstract:

To determine which members of the newly discovered gene family of angiopoietins are expressed in human tumours by Reverse Transcription-Polymerase Chain Reaction (RT-PCR); to identify the cellulr sources of angiopoietins in tumours by In Situ Hybridisation (ISH); to study the expression of Tie2 in tumours by RT-PCR and ISH; to compare the expression patterns of angiopoietins and Tie2, and determine the role of these molecules in tumour angiogenesis.

List of Research Outputs

Au W.Y., Chan C.P., Chung L.P. and Choy C.K.W., Primary B-Cell lymphoma and lymphoma-like lesions of the uterine cervix, American Journal of Hematology. 2003, 73: 176-179.

Chim J.C.S., Shek T.W.H., Chung L.P. and Liang R.H.S., Multiple lymphomatous polyposis in lymphoma of colon, Journal of Clinical Oncology. 2003, 953 – 955.

Choi K.C., Wong M.P., Tam Y.S.I., Lam C.L., Lam W.K., Wang E., Chau W.S., Chiu S.W. and Chung L.P., Angiogenic activity and angiogenesis-related factor expression in non-small cell lung cancers, Proceedings of the 10th Hong Kong International Cancer Congress, 19-21 November 2003.

Hon C., Au W.Y., Chung L.P. and Choy C.K.W., Allogeneic stem cell transplantation for therapy related leukemia after bilateral retinoblastoma in a girl with del(13q) syndrome, Annals of Hematology. 2004, 75: 115-6.

Lam C.L., Wong M.P., Lam W.K., Chung L.P., Chiu S.W., Chau W.S., Ho K.K. and Minna J.D., Quantitative comparison of expression of nicotinic acetylcholine receptor (nAChR)subunits between non-small cell lung cancers (NSCLCs) arising in life-time never-smokers and smokers in Chinese patients , Research Postgraduate Symposium Presentation, The University of Hong Kong. 2003, Abstract Book.

Lam D.C.L., Wong M.P., Chung L.P., Girard L., Lam W.K. and Minna J.D., Expression profiling with microarray in studying differential gene expression in different primary lung cancer cell lines newly established from local Chinese patients, 9th Medical Research Conference, Medical Science Group, The University of Hong Kong. 2004, Abstract Book: RC-15.

Lam D.C.L., Wong M.P., Lam W.K., Chung L.P., Chiu S.W., Chau W.S., Ho K.K. and Minna J.D., Quantitative comparison of expression of nicotinic acetylcholine receptor (nAChR)subunits between non-small cell lung cancer (NSCLC) arising in life-time never-smokers in Chinese patients , Lung Cancer. 2003, 41 (suppl 2): S89.

Lam D.C.L., Wong M.P., Lam W.K., Chung L.P., Chiu S.W., Chau W.S., Ho K.K. and Minna J.D., Quantitative comparison of nicotinic acetylcholine receptor subunit expression level reveals differences in non-small cell lung cancer arising fron life-time never-smokers and smokers, 9th Medical Research Conference, Medical Science Group, The University of Hong Kong. 2004, Abstract Book: RC-14.

Li T.C., Wong M.P., Tam Y.S.I., Lam C.L., Lam W.K., Wang E., Chau W.S., Chiu S.W. and Chung L.P., Promoter hypermethylation of MGMT, RARB, DAPK and CDH13 genes in non-small cell lung cancers (NSCLC) in Hong Kong, Proceedings of the 10th Hong Kong International Cancer Congress, 19-21 November 2003.

Zhu H., Wong M.P., Lam C.L., Cai W.W., Chau W.S., Wang E., Lam W.K., Chiu S.W. and Chung L.P., Genome-wide screening of chromosomal imbalance in NSCLC by array-based comparative genomic hybridization (array-CGH), Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Researcher : Collins RJ

Project Title:	Molecular genetics in gastrointestinal polyposis syndrome
Investigator(s):	Dr. Collins R.J., Dr. Chung L.P., Dr. Ho J.W.C., Dr. Yuen S.T.
Department:	Pathology (Hospital Pathology Services Morbid Anatomy/Cytology)
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	07/1998

Abstract:

To define the molecular genetic changes in three polyposis syndromes; to establish methods for efficiency screening of at-risk individuals within these families.

List of Research Outputs

Mok T.M.Y., Lao T.T.H., Collins R.J., Leung T.Y., Leung P.Y., Chan D.T.M., Wong R.W.S. and Lau W.C.S., Cervical dysplasia in patients with systemic lupus erythematosus, 7th International Congress on SLE and Related Condidtions, New York. 2004.

Mok T.M.Y., Lao T.T.H., Collins R.J., Leung T.Y., Leung P.Y., Lo Y., Chan D.T.M., Wong R.W.S. and Lau W.C.S., Cervical dysplasia in patients with systemic lupus erythematosus, The Hong Kong Practitioner. 2004, 2: RI-05.

Researcher : Guo D

List of Research Outputs

Chan T.L., Wong C.W., Chan A.S.W., Guo D., Ho J.W.C., Yuen S.T. and Leung S.Y., BRAF and KRAS mutations in mismatch repair deficient colorectal cancer , Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Guo D., Yuen S.T., Ho J.W.C., Chan A.S.Y., Chen X. and Leung S.Y., PLA2G2A expression and its clinical significance in colorectal tumors, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Researcher : Hau KL

List of Research Outputs

Beh S.L. and Hau K.L., The development of forensic services in Hong Kong, History Book of the Indo-Pacific Association of Law, Medicine and Science (INPALMS), Edited by Salgado, M.S.L., Tatsuno, Y. & Tsunenari, S. Sri Lanka, The Medico-Legal Society of Sri Lanka, 2003, 149-152.

Researcher : Hawkins BR

Project Title:	Maintenance of a bone marrow donor registry
Investigator(s):	Dr. Hawkins B.R.
Department:	Pathology (Hospital Pathology Services Immunology)
Source(s) of Funding:	Hong Kong Marrow Match Foundation
Start Date:	01/1992

Abstract:

To study maintenance of a bone marrow donor registry.

List of Research Outputs

Kwok J.S.Y., Leung A.Y.H., Lie A.K.W., Lee T.L., Lau Y.L., Chu P., Jones B.M., Hawkins B.R. and Liang R.H.S., Allo-reactive lymphocytes: Antireceipient helper and cytotoxic T-cell frequencies in bone marrow transplantation, Bone Marrow Transplantation. 2004, 34: 207-213.

Researcher : Higgins DA

List of Research Outputs

Fong S.W., Chan K.W., Zhang J.C.L., Higgins D.A., Liang Y. and Chan S.Y., Cardiac expression of NAP79, a new membeer of Nucleosome Assembly Proteins, 2004 Sir Edward Youde Memorial Fund Postgraduate Conference, Model Organism Research and Human Diseases, HK, 14-15 June 2004. 34.

Fong S.W., Chan K.W., Liang Y., Higgins D.A. and Chan S.Y., Expression Study of NAP79, a new member of Nucleosome Assembly Proteins, 1^st Joint Meeting of the British and French Societies for Developmental Biology, Nice, France, 13-16 September 2003.

Researcher : Ho JCY

List of Research Outputs

Wong B.C.Y., Lam S.K., Wong R.W.M., Chen J.S., Zheng T., Feng R., Lai K.C., Hu H.C., Yuen S.T., Leung S.Y., Fong D.Y.T., Ho J.C.Y., Ching C.K. and Chen J.S., Helicobacter pylori Eradication to Prevent Gastric Cancer in a High-Risk Region of China: a Randomized Controlled Trial, The Journal of American Medical Association. 2004, 291(2): 187-194.

Researcher : Hu X

List of Research Outputs

Chen Y.W., Liang A.C.T., Au W.Y., Chu K.M., Wong K.Y., Hu X., Lu L., Tang J.C.O., Chan K.W., Beh S.L., Kwong Y.L., Liang R.H.S. and Srivastava G., Multiple BCL6 translocation partners in individual cases of gastric lymphoma, Blood. 2003, 102: 1931-1932.

Researcher : Huang F

Project Title:	The roles of regulatory dendritic cells in peripheral tolerance and autoimmunity
Investigator(s):	Dr. Huang F.P., Dr. MacPherson G.G.
Department:	Pathology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	09/2001

Abstract:

To create in vivo models which mimic and auto- or host-reactive environment; to visualise the fate of potential self-reactive T cells in normal and in autoimmune-prone mice; to study the role of DCreg in the generation of Treg cells; to study DC functions in autoimmune models; to generate DCreg and Treg for the treatment of autoimmune diseases.

Project Title:	Functional conditioning of dendritic cells for DC-based tumor vaccine
Investigator(s):	Dr. Huang F.P., Prof. Ng I.O.L., Dr. Tian L.
Department:	Pathology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	01/2003

Abstract:

Dendritic cell (DC)-based tumor vaccine is a newly developed therapeutic approach for cancer treatment. It aims to promote specific immunity to cancer cells within tumor bearing individuals. Recent studies reveal that DC are not a homogenous population, and their ability to provide activation signals can vary significantly between different DC subtypes, lineages or maturity. The types and functional conditions, hence the immunogenic 'quality', of the DC employed are understandably essential. This project is to examine critically the roles of co-stimulatory molecules in DC-based tumor vaccines. The main objective is to develop immunologically active and programmable DC-tumor vaccines with high efficacy useful in cancer therapies.

Project Title:	Immunotherapy by adoptive transfer of T regulatory cells in a mouse model of systemic lupus erythematosus
Investigator(s):	Dr. Huang F.P., Dr. Tian L.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	01/2003
Completion Date:	12/2003

Abstract:

To study the immunological relevance of age-dependency of T regulatory cells in normal and autoimmune mouse models; to test the hypothesis that lack of naturally occuring T regulatory cells is the cause for autoimmune disorders in a mouse model with spontaneous, age-dependent lupus disease; to determine the therapeutic potential of T regulatory cells in the lupus model by adoptive transfer of the cells.

Project Title:	Mechanisms for the induction and regulation of autoimmune responses by dendritic cells and T regulatory cells in systemic lupus erythematosus
Investigator(s):	Dr. Huang F.P., Dr. Tian L., Dr. Wu A.Y.Y.
Department:	Pathology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	12/2003

Abstract:

To study the mechanisms for induction and regulation of autoimmune responses; to study effects of cell death on the induction of anti-nuclear antibody production, in normal and in autoimmune-prone mice; to test the hypothesis that uptake of necrotic or apoptotic dying cells by dendritic cells may differentially regulate autoimmune responses in vivo; to determine the roles, and mechanisms for generation, of the naturally occurring T regulatory cells in autoimmune and non-autoimmune mice.

Project Title:	Mechanisms for the induction and regulation of autoimmune responses by dendritic cells and T regulatory cells in systemic lupus erythematosus
Investigator(s):	Dr. Huang F.P., Dr. Tian L., Dr. Wu A.Y.Y.
Department:	Pathology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	12/2003

Abstract:

List of Research Outputs

Chan S., Huang F., Chim J.C.S., Ma E.S.K., Law H.K.W., Lau Y.L. and Chan G.C.F., Dendritic cells (DBS) can be induced from marrows of both newly diagnosed lymphoid and myeloid leukaemia but they demonstrated different morphological and immunological behaviours, Joint Annual Scientific Meeting 2003 of the Hong Kong Paediatric Society and Hong Kong Paediatric Nurses Association Limited, QEH, Hong Kong, 13 December 2003. 119.

Huang F. and MacPherson G.G., Dendritic cells and oral transmission of prion diseases, Advanced Drug Delivery Reviews. 2004, 56(6): 901-913.

Huang F., Ma L., Wu A.Y.Y., Tian L., Chan K.W., Chan A.K., Lam A.C., Lo C.K., Ko K.H., To K.W., Chik S., Kam S.K., Fong I., Li X.S., Ng M.H. and MacPherson G.G., Systemic autoimmune disease induced by dendritic cells that have captured dying cells, Proceedings of the Frontiers in Biomedical Research, Hong Kong. 12 December 2003.

Ma L., Chan A.K., Lam A.C., Chan K.W., Lo C.K., Ko K.H., To K.W., Wu A.Y.Y., Tian L., Ng M.H. and Huang F., The role of dendritic cells in the induction of systemic autoimmune disease, Proceedings of the 8th Research Postgraduate Symposium, Hong Kong, 13 December 2003.

Tian L., Yuan Z.W., Li X.S., Huang F., Lamb J.R., Dallman M. and Tam P.K.H., Orthotopic small bowel but not heterotopic cardiac allograft recipients treated with an unique FK506 regimen contributes to the induction of regulatory T cells, Basic Sciences Symposium 2003: Transplantation Immunology in the Real World, Colorado, U.S.A, 14-17 September 2003.

Wong O.H., Huang F. and Chiang A.K.S., Role of dendritic cell in the immune regulation of primary epstein-barr virus infection, 8^th Research Postgraduate Symposium, Hong Kong, 13 December 2003. 76.

Researcher : Ip PPC

List of Research Outputs

Xue W., Khoo U.S., Ngan H.Y.S., Chan Y.K., Ip P.P.C., Tsao G.S.W. and Cheung A.N.Y., c-mos immunoreactivity aids in the diagnosis of gestational trophoblastic lesions, International Journal of Gynecological Pathology. 2004, 23(2): 145-150.

Researcher : Jones BM

Project Title:	Cytokines in patients with coronavirus pneumonia and analysis of a laboratory database for SARS
Investigator(s):	Dr. Jones B.M., Dr. Ma E.S.K.
Department:	Pathology (Hospital Pathology Services Immunology)
Source(s) of Funding:	SARS Research Fund
Start Date:	07/2003
Completion Date:	12/2003

Abstract:

To understand the role of cytokines, particularly those involved in inflammation, in SARS pathogenesis; to develop a cytokine-based assay for identification of T-cells sensitized by the SARS coronavirus for early diagnosis of infected patients and identification of candidate vaccines; to establish and analyse a database of routine laboratory evaluations in SARS and suspected SARS patients at Queen Mary Hospital.

List of Research Outputs

Jones B.M., Ma E.S.K., Peiris J.S.M., Wong P.C., Ho J.C.M., Lam B., Lai K.N. and Tsang K.W.T., Prolonged disturbances of in vitro cytokine production in patients with severe acute respiratory syndrome (SARS) treated with ribavirin and steroids, Clinical & Experimental Immunology. 2004, 135: 467-473.

Kwok J.S.Y., Leung A.Y.H., Lie A.K.W., Lee T.L., Lau Y.L., Chu P., Jones B.M., Hawkins B.R. and Liang R.H.S., Allo-reactive lymphocytes: Antireceipient helper and cytotoxic T-cell frequencies in bone marrow transplantation, Bone Marrow Transplantation. 2004, 34: 207-213.

Researcher : Khoo US

Project Title:	Detection of breast cancer cells in fine needle aspirates by methylation-specific polymerase-chain-reaction
Investigator(s):	Dr. Khoo U.S.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2002

Abstract:

To find out if methylation analysis could be a useful adjunct to identification of breast cancer cells in fine needle aspiration cytology (FNAC) samples taken from lesions of the breast.

Project Title:	Genetic polymorphism study of the immense response genes in relation to susceptibility, clinical outcome and treatment response of SARS
Investigator(s):	Dr. Khoo U.S., Dr. Chan K.Y.K., Prof. Chan L.C., Dr. Chan E.Y.T., Dr. Chen R.Y.L., Dr. Cheung A.N.Y., Dr. Huang F.P., Prof. Lau Y.L., Dr. Ma E.S.K., Prof. Peiris J.S.M., Dr. Yip S.P.
Department:	Pathology
Source(s) of Funding:	SARS Research Fund
Start Date:	07/2003
Completion Date:	12/2003

Abstract:

To assess the influence of single nucleotide polymorphisms of the immune response genes likely to mediate viral entry, antigen uptake and cytokine response to the SARS coronavirus (CoV).

Project Title:	Association of the pro-inflammatory and anti-inflammatory cytokine gene polymorphism to breast cancer susceptibility
Investigator(s):	Dr. Khoo U.S., Dr. Chan K.Y.K., Dr. Cheung A.N.Y., Dr. Yip S.P.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2003

Abstract:

To determine whether the genetic variants of the pro-inflammatory and anti-inflammatory cytokines may (a) contribute towards breast cancer susceptibility or (b) influence prognosis; to investigate whether the joint effects of several of these alleles and/or in combination of specific environmental factors may contribute towards a stronger association.

List of Research Outputs

Au W.Y., Wong R.W.M., Khoo U.S. and Liang R.H.S., Concurrent aggressive gastrointestinal stromal tumour (GIST) and Burkitt’s lymphoma., Journal of Clinical Oncology. 2003, 21: 1417 – 1418.

Chan K.Y.K., Song Y., Mak W., Cheung A.N.Y., Peiris J.S.M., Yip S.P., Tin V., Liu W., Xue W.C., Chan L.C., Tam P.K.H., Ngan H.Y.S. and Khoo U.S., Polymorphisms of the ACE2 and CD13 (ANPEP) genes, and susceptibility to SARS coronavirus infection in Chinese from Hong Kong., The Human Genome organisation Meeting, April 2004, Berlin, Germany. 2004, Abstract No. 159.

Chan Y.K., Shen D.H., Li S.S., Khoo U.S., Ngan H.Y.S., Xue W. and Cheung A.N.Y., Differential Expression and Methylation of p33/ING1b in Ovarian Tumors, Proceedings of the 94th Annual Meeting of the American Association for Cancer Research, Washington, USA, 11-14 July 2003.

Chan Y.K., Song Y.Q., Mak W., Cheung A.N.Y., Peiris J.S.M., Yip S.P., Tin V., Liu W., Xue W., Chan L.C., Tam P., Ngan H.Y.S. and Khoo U.S., Polymorphisms of the ACE2 and CD13 genes, and susceptibility to SARS coronavirus infection in Chinese from Hong Kong, Abstract for Human Genome Organization (HUGO) Meeting, April 4-7, 2004, Berlin, Germany. 2004.

Chan Y.K., Khoo U.S., Chan K.H.K., Ngan H.Y.S., Li S.S., Xue W., Chiu P.M. and Cheung A.N.Y., Reduced GSTP1 immunoreactivity in endometrial carcinomas in association with methylation status and genetic profiles, Proceedings of the 94th Annual Meeting of the American Association for Cancer Research, Washington, USA, 11-14 July 2003.

Cheung A.N.Y., Szeto E.F., Ng K.M., Fong K.W., Ang Chan E.Y., Tsun O.K.L., Khoo U.S., Chan Y.K. and Ng W.Y., Atypical squamous cells of undetermined significance on cervical smears: follow-up study of an Asian screening population, Cancer Cytopathology. 2004, 102(2): 74-80.

Cheung A.N.Y., Szeto E.F., Leung B.S.Y., Khoo U.S. and Ng W.Y., Liquid-based cytology and conventional cervical smears: a comparison study in an Asian screening population, Cancer. 2003, 99(6): 331-335.

Cheung A.N.Y., Khoo U.S., Lai C.Y.L., Chan Y.K., Xue W., Cheng D.K.L., Chiu P.M., Tsao G.S.W. and Ngan H.Y.S., Metastatic trophoblastic disease after an initial diagnosis of partial hydatidiform mole: genotyping and chromosome in situ hybridization analysis, Cancer. 2004, 100(7): 1411-1417.

Cheung A.N.Y., Khoo U.S., Xue W. and Shen D.H., Recent advances in the research of gestational trophoblastic diseases, 妊娠滋??胞病?的研究?展, Chinese Journal of Pathology. 中?病理??志 , 2003, 32(4): 373-375.

Chiu P.M., Liu V.W.S., Ngan H.Y.S., Khoo U.S. and Cheung A.N.Y., Detection of mitochondrial DNA mutations in gestational trophoblastic disease, Human Mutation. 2003, 22(2): 177.

Khoo U.S., Koo W.T., Chan Y.K. and Cheung A.N.Y., DNA-based detection of breast cancer in fluid arising from Fine-Needle-Aspiration Cytology samples, Proceedings of the 94th Annual Meeting of the American Association for Cancer Research, Washington, USA, 11-14 July 2003.

Khoo U.S., Genetic Susceptibility to SARS: Escape mechanism via the DC-SIGN and DC-SIGN-related receptors, Abstracts for Hong Kong SARS Forum and Hospital Authority Convention 2004, Hong Kong, 8-11 May 2004.

Khoo U.S., Molecular Oncology ABC: Breast, Proceedings of the 10th Hong Kong International Cancer Congress, 19-21 November 2003.

Khoo U.S., Pathology of breast cancer and its contribution to management, Proceedings of the New Challenges in Health Care, 10th Anniversary of Hong Kong Academy of Medicine, 28 November 2003.

Khoo U.S., Chan Y.K., Yip S.P., Peiris M., Tin V., Liu W., Xue W., Ngan H.Y.S., Chan L.C. and Cheung A.N.Y., The tandem repeat polymorphism of the DC-SIGNR gene is associated with susceptibility to SARS coronavirus infection in Chinese from Hong Kong, Proceedings of the Human Genome Organization (HUGO) Meeting, April 4-7, 2004, Berlin, Germany. 2004.

Lai C.Y.L., Chan Y.K., Khoo U.S., Ngan H.Y.S., Xue W., Chiu P.M., Tsao G.S.W. and Cheung A.N.Y., Analysis of gestational trophoblastic disease by genotyping and chromosome in situ hybridization, Modern Pathology. 2004, 17: 40-48.

Leung T.W., Xue W., Cheung A.N.Y., Khoo U.S. and Ngan H.Y.S., Proliferation to apoptosis ratio as a prognostic marker in adenocarcinoma of uterine cervix, Gynecologic Oncology. 2004, 92(3): 866-872.

Shen D., Khoo U.S., Ngan H.Y.S., Ng T.Y., Chau M.T., Xue W. and Cheung A.N.Y., Coexisting epithelioid trophoblastic tumor and choriocarcinoma of the uterus following a chemoresistant hydatidiform mole, Archives of Pathology and Laboratory Medicine. 2003, 127(7): e291–e293.

Xue W., Khoo U.S., Ngan H.Y.S., Chan Y.K., Chiu P.M., Tsao G.S.W. and Cheung A.N.Y., Minichromosome maintenance protein 7 expression in gestational trophoblastic disease: correlation with Ki67, PCNA and clinicopathological parameters, Histopathology. 2003, 43: 485-490.

Xue W., Khoo U.S., Ngan H.Y.S., Chan Y.K., Ip P.P.C., Tsao G.S.W. and Cheung A.N.Y., c-mos immunoreactivity aids in the diagnosis of gestational trophoblastic lesions, International Journal of Gynecological Pathology. 2004, 23(2): 145-150.

Yang C., Ngan H.Y.S., Khoo U.S., Chan Y.K., Chiu P.M., Chan Q.K.Y., Xue W. and Cheung A.N.Y., Single nucleotide polymorphisms (SNPS) in the coding sequence of follicle stimulating hormone receptor (FSHR) is associated with ovarian cancer susceptibility, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Yang H., Liu V.W.S., Wang Y., Chan Y.K., Tsang P.C.K., Khoo U.S., Cheung A.N.Y. and Ngan H.Y.S., Detection of hypermethylated genes in tumor and plasma of cervical cancer patients, Gynecologic Oncology. 2004, 93(2): 435-440.

Researcher : Ko KH

List of Research Outputs

Huang F., Ma L., Wu A.Y.Y., Tian L., Chan K.W., Chan A.K., Lam A.C., Lo C.K., Ko K.H., To K.W., Chik S., Kam S.K., Fong I., Li X.S., Ng M.H. and MacPherson G.G., Systemic autoimmune disease induced by dendritic cells that have captured dying cells, Proceedings of the Frontiers in Biomedical Research, Hong Kong. 12 December 2003.

Ma L., Chan A.K., Lam A.C., Chan K.W., Lo C.K., Ko K.H., To K.W., Wu A.Y.Y., Tian L., Ng M.H. and Huang F., The role of dendritic cells in the induction of systemic autoimmune disease, Proceedings of the 8th Research Postgraduate Symposium, Hong Kong, 13 December 2003.

Shen L., Liang A.C.T., Lu L., Au W.Y., Wong K.Y., Tin P.C., Chan K.W., Ko K.H., Chen W.Y.W., Beh S.L., Shimizu N., Tsuchiyama T., Tang J.C.O., Kwong Y.L., Liang R.H.S. and Srivastava G., Aberrant BCL10 nuclear expression in nasal NK/T-cell lymphoma, Blood. 2003, 102(4): 1553-1554.

Researcher : Kong CT

List of Research Outputs

Kong C.T., Chan L.C. and Sham M.H., Mll-Een affects early lineage commitment of embryonic stem cells and cause maturation arrest of myeloid progenitors, FASEBSummer Research Conference on Hematologic Malignancies 26-31 July 2003, Vermount, USA. 2003.

Researcher : Kwok JSY

List of Research Outputs

Kwok J.S.Y., Leung A.Y.H., Lie A.K.W., Lee T.L., Lau Y.L., Chu P., Jones B.M., Hawkins B.R. and Liang R.H.S., Allo-reactive lymphocytes: Antireceipient helper and cytotoxic T-cell frequencies in bone marrow transplantation, Bone Marrow Transplantation. 2004, 34: 207-213.

Ma E.S.K., Kwok J.S.Y., Li Y.H. and Chan L.C., Clinical significance of ADAMTS13 mutational analysis in relapsing TTP with severe vWF-cleaving protease deficiency, Blood. 2003, 102(11) Part 1: 802a.

Ma E.S.K., Kwok J.S.Y., Li Y.H. and Chan L.C., Clinical significance of ADAMTS13 mutational analysis in relapsing TTP with severe vWF-cleaving protease deficiency, Proceedings of the American Society of Hematology, San Diego, USA, 6-9 December 2003. Abstract No. 2970.

Researcher : Lam CCK

List of Research Outputs

Au W.Y., Lam C.C.K. and Kwong Y.L., Successful treatment of acquired factor VIII inhibitor with cyclosporine, Haemophilia. 2004, 10: 98-100.

Au W.Y., Lam C.C.K., Lie A.K.W., Pang A.W.K. and Kwong Y.L., T-cell large granular lymphocyte leukemia of donor origin after allogeneic bone marrow transplantation, Americal Journal of Clinical Pathology. 2003, 120: 626-30.

Au W.Y., Cheung W.C., Lam C.C.K. and Kwong Y.L., Two factor XI gene mutations in a Chinese family with factor XI deficiency, American Journal of Hematology. 2003, 74: 136-8.

Au W.Y., Lam C.C.K., Lam C.C.K., Lam C.C.K., Cheung W.C. and Kwong Y.L., Two novel factor X deficiency mutations in a Chinese family, Annals of Hematology. 2004, 83: 304-306.

Lee C.K., Ma E.S.K., Shek T.W.H., Lam C.C.K., Au W.Y., Wan T.S.K. and Chan L.C., Plasmablastic transformation of multiple myeloma, Human Pathology. 2003, 34(7): 710-714.

Lee C.K., Ma E.S.K., Tang M., Lam C.C.K., Lin C.K. and Chan L.C., Prevalence and specificity of clinically significant red cell alloantibodies in Chinese women during pregnancy - a review of cases from 1997 to 2001, Transfusion Medicine. 2003, 13(4): 227.

Ma S.Y., Au W.Y., Chim J.C.S., Lie A.K.W., Lam C.C.K., Tse E.W.C., Leung A.Y.H., Liang R.H.S. and Kwong Y.L., Fludarabine, mitoxantrone and dexamethasone in the treatment of indolent B-and T-cell lymphoid malignancies Chinese patients, British Journal of Haematology. 2004, 124: 754-761.

Wan T.S.K., Ma E.S.K., Lam C.C.K., Chan L.C., Lee K.K. and Au W.Y., Deletion 9q as the sole karyotypic abnormality in myelocytic disorders: a new case of myelodysplastic syndrome and its prognostic implications in acute myelocytic leukemia, Cancer Genetics and Cytogenetics. 2003, 145(2): 184-186.

Researcher : Lam CL

List of Research Outputs

Choi K.C., Wong M.P., Tam Y.S.I., Lam C.L., Lam W.K., Wang E., Chau W.S., Chiu S.W. and Chung L.P., Angiogenic activity and angiogenesis-related factor expression in non-small cell lung cancers, Proceedings of the 10th Hong Kong International Cancer Congress, 19-21 November 2003.

Li T.C., Wong M.P., Tam Y.S.I., Lam C.L., Lam W.K., Wang E., Chau W.S., Chiu S.W. and Chung L.P., Promoter hypermethylation of MGMT, RARB, DAPK and CDH13 genes in non-small cell lung cancers (NSCLC) in Hong Kong, Proceedings of the 10th Hong Kong International Cancer Congress, 19-21 November 2003.

Zhu H., Wong M.P., Lam C.L., Cai W.W., Chau W.S., Wang E., Lam W.K., Chiu S.W. and Chung L.P., Genome-wide screening of chromosomal imbalance in NSCLC by array-based comparative genomic hybridization (array-CGH), Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Researcher : Law BFF

List of Research Outputs

Law B.F.F., Srivastava G., Wong K.Y., Chen W.Y.W., Liang A.C.T., Lam K.Y., Law S.Y.K., Wong J. and Tang J.C.O., A novel tumor transforming gene at 7q22, GAESCC1, is frequently amplified and overexpressed in primary esophageal squamous cell carcinomas, Abstract of the Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Researcher : Lee CK

List of Research Outputs

Lee C.K., Ma E.S.K., Shek T.W.H., Lam C.C.K., Au W.Y., Wan T.S.K. and Chan L.C., Plasmablastic transformation of multiple myeloma, Human Pathology. 2003, 34(7): 710-714.

Lee C.K., Ma E.S.K., Tang M., Lam C.C.K., Lin C.K. and Chan L.C., Prevalence and specificity of clinically significant red cell alloantibodies in Chinese women during pregnancy - a review of cases from 1997 to 2001, Transfusion Medicine. 2003, 13(4): 227.

Researcher : Lee JSK

List of Research Outputs

Ambekar C.S., Lee J.S.K., Cheung B.M.Y., Chan L.C., Liang R.H.S. and Kumana C.R., Chloramphenicol succinate, a competitive substrate and inhibitor of succinate dehdrogenase : possible reason for its toxicity., Toxicology in Vitro. 2004, 18: 441-447.

Researcher : Lee MF

List of Research Outputs

Chan D.W., Lee M.F. and Ng I.O.L., Characterization of H-cadherin expression in human hepatocellular carcinoma, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Ho M.K.S., Lee M.F., Chan C.K.L. and Ng I.O.L., Allelic alterations in nontumorous liver tissues and corresponding hepatocellular carcinomas from Chinese patients, Human Pathology. 2003, 34(7): 699-705.

Ng I.O.L., Chan K.L., Shek T.W.H., Lee M.F., Fong D.Y.T., Lo C.M. and Fan S.T., High frequency of chimerism in transplanted livers (Abstract), The 5th Annual Scientific Meeting, Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 3 January 2004.

Wong C.M., Ching Y.P., Lee M.F., Jin D. and Ng I.O.L., Genetic and epigenetic alterations of DLC-1 gene in hepatocellular carcinoma, Cancer Research. 2003, 63: 7646-7651.

Researcher : Leung BSY

List of Research Outputs

Cheung A.N.Y., Szeto E.F., Leung B.S.Y., Khoo U.S. and Ng W.Y., Liquid-based cytology and conventional cervical smears: a comparison study in an Asian screening population, Cancer. 2003, 99(6): 331-335.

Researcher : Leung HY

List of Research Outputs

Leung H.Y., Ching Y.P., Wong C.M., Ng D.C.H., Jin D. and Ng I.O.L., Characterization of novel putative tumor suppressor gene, DLC2 (deleted in liver cancer 2), in hepatocellular carcinoma, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Researcher : Leung SY

Project Title:	Genetic instability in early onset colorectal cancer in Hong Kong Chinese
Investigator(s):	Dr. Leung S.Y., Dr. Chung L.P., Dr. Ho J.W.C., Prof. Wyllie A.H., Dr. Yuen S.T.
Department:	Pathology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	10/2000

Abstract:

To examine a large cohort o fyoung patients for microsatellite instability (MSI) and mismatch repair (MMR) gene mutation. Results obtained on MMR gene will help to formulate strategy for mutation detection, prophylactic screening and management of young MSI colorectal cancer (CRC) patients and particularly their family members.

Project Title:	Haplotype analysis of recurrent germline mutations in DNA mismatch repair genes in Hong Kong Chinese - a search for founder mutations
Investigator(s):	Dr. Leung S.Y., Dr. Yuen S.T.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2001
Completion Date:	10/2003

Abstract:

To perform haplotype analysis on unrelated kindreds with the same germline MMR gene mutation, to explore the possibility of a founder effect.

Project Title:	Identification of novel differentially expressed genes in gastric cancer and their functional characterisation
Investigator(s):	Dr. Leung S.Y., Dr. Chu K.M., Prof. Kung H.F., Dr. Lin M.C.M., Dr. Yuen S.T.
Department:	Pathology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	12/2001

Abstract:

To identify and isolate partial cDNA sequences of genes that are differentially-expressed in various stages of gastric carcinogenesis using combined supression subtractive hybridisation and cDNA microarrays; to study cloning of full length cDNA sequences for novel genes; to study expression analysis of these genes in the mRNA and protein level in a large panel of gastric cancers, and examine their relationaship with clinico-pathological parameters, association with aetiological agents, molecular genetic changes, prognosis, response to treatment and their potential use as tujour markers; to study functional characterisation of these novel genes, with regard to their effects in cell division, mitotic activity, tranforming activity, tumourigenicity, roles in cell cycle control, apoptosis, and relationship with other signal trnsduction pathways.

Project Title:	High resolution mapping of chromosomal aberrations by cDNA microarray-based comparative genomic hybridisation and their correlation with gene expression profile of gastric adenocarcinoma
Investigator(s):	Dr. Leung S.Y., Dr. Chen X., Dr. Chu K.M., Dr. Lin M.C.M., Dr. So S., Dr. Yuen S.T.
Department:	Pathology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	11/2002

Abstract:

The project attempts to achieve a high resolution mapping of the chromosomal gains and losses in primary gastric cancer samples by hybridisation of genomic DNA to a microarray containing 44,000 human cDNA clones. The changes of DNA copy number will then be correlated with gene expression profiles already available in the gastric cancer samples, generated using the same cDNA microarray. New oncogenes or tumour suppressor genes may be identified in these regions that may constitute new targets for diagnosis, prognostication and pathway specific therapeutic strategy.

Project Title:	Identification of BRAF mutation in various stages of colorectal carcinogenesis and its relationship with KRAS mutation
Investigator(s):	Dr. Leung S.Y., Dr. Yuen S.T.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2002

Abstract:

To examine the biological relationship of BRAF mutation with KRAS mutation. The association of mutations in these two genes with clinico-pathological features, molecular parameters and prognosis will be sought.

Project Title:	Delineation of prognostic biomarkers in gastric cancer using cDNA microarray data, validation in independent dataset and their functional characterisation
Investigator(s):	Dr. Leung S.Y., Dr. Chen X., Dr. Chu K.M., Prof. Kung H.F., Dr. Lin M.C.M., Dr. So S., Dr. Yuen S.T.
Department:	Pathology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	11/2003

Abstract:

To identify a list of genes that significantly predict tumour behaviour and patient outcome in a cohort of 90 gastric adenocarcinoma patients studied by cDNA microarray; to validate the prognostic significance of the top 50 survival genes in a large independent cohort of gastric adenocarcinoma patients using a combination of real-time quantitative RT-PCR, in-situ hybridisation and immunohistochemistry performed in high-density tissue microarray; to carry out functional characterisation of the survival genes using various cell culture and animal models.

Project Title:	Delineation of prognostic biomarkers in gastric cancer using cDNA microarray data, validation in independent dataset and their functional characterisation
Investigator(s):	Dr. Leung S.Y., Dr. Chen X., Dr. Chu K.M., Prof. Kung H.F., Dr. Lin M.C.M., Dr. So S., Dr. Yuen S.T.
Department:	Pathology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	11/2003

Abstract:

Project Title:	Detail study of relationship of BRAF and KRAS mutation with microstatellite instability in colorectal cancer
Investigator(s):	Dr. Leung S.Y., Dr. Yuen S.T.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2003

Abstract:

To examine in detail the incidence of BRAF and KRAS mutation in a large series of MSI colorectal cancer with detail characterization of germine MMR gene mutation and MLH1 promoter methylation.

List of Research Outputs

Chan A.S.W., Tsui W.Y., Chen X., Chu K.M., Chan T.L., Chan A.S.Y., Li R., So S., Yuen S.T. and Leung S.Y., Down-regulation of ID4 by promoter hypermethylation in gastric adenocarcinoma, Proceedings of the International Conference on Applied Genomics, 9th European Society for Analytical Cellular Pathology meeting and 16th International Society for Diagnostic Quantitative Pathology meeting, Netherlands, 2003.

Chan A.S.W., Tsui W.Y., Chen X., Chu K.M., Chan T.L., Chan A.S.Y., Li R., So S., Yuen S.T. and Leung S.Y., Downregulation of ID4 by promoter hypermethylation in gastric adenocarcinoma, Oncogene. 2003, 22(44): 6946-6953.

Chan A.S.W., Chu K.M., Yuen S.T. and Leung S.Y., High-throughput quantification of hMLH1 promoter methylation using PyrosequencingTM technology , Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Chan S.C., Cheung S.T., Leung S.Y., Liu C.L., Fan S.T. and Lo C.M., De novo sarcoma of donor origin in a liver allograft determined by microsatellite analysis: a short report, Liver Transplantation. 2004, 10(2): 320-323.

Chan T.L., Zhao W., Cancer Genome Project .., Leung S.Y. and Yuen S.T., BRAF and KRAS mutations in colorectal hyperplastic polyps and serrated adenomas, Cancer Research. 2003, 63: 4878-4881.

Chan T.L., Wong C.W., Chan A.S.W., Guo D., Ho J.W.C., Yuen S.T. and Leung S.Y., BRAF and KRAS mutations in mismatch repair deficient colorectal cancer , Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Chan T.L., Chan Y.W., Ho J.W.C., Chan C., Chan A.S.Y., Chan E., Lam P.W.Y., Tse C.W., Lee K.C., Lau C.W., Gwi E., Leung S.Y. and Yuen S.T., MSH2 c.1452-1455delAATG is a founder mutation and an important cause of hereditary nonpolyposis colorectal cancer in the southern Chinese population, The American Journal of Human Genetics. 2004, 74(5): 1035-1042.

Chen X., Leung S.Y., Yuen S.T., Chu K.M., Ji J., Li R., Chan A.S.Y., Law S.Y.K., Troyanskaya O.G., Wong J., So S., Botstein D. and Brown P.O., Variation in gene expression patterns in human gastric cancers, Molecular Biology of the Cell. 2003, 14(8): 3208-3215.

Guo D., Yuen S.T., Ho J.W.C., Chan A.S.Y., Chen X. and Leung S.Y., PLA2G2A expression and its clinical significance in colorectal tumors, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Ko S., Chu K.M., Luk J.M.C., Wong W.Y., Leung S.Y., Yuen S.T. and Wong J., Expression of CDX2 and LI-cadherin in intestinal metaplasia and adenocarcinoma of the stomach, 95th Annual Meeting of the American Association for Cancer Research, Orlando, U.S.A., 27-31 March 2004.

Leung S.Y., Gastric cancer – towards identification of markers for early detection and prediction of germline susceptibility, Proceedings of the 10th Hong Kong International Cancer Congress, Hong Kong, 19-21 November 2003.

Wong B.C.Y., Lam S.K., Wong R.W.M., Chen J.S., Zheng T., Feng R., Lai K.C., Hu H.C., Yuen S.T., Leung S.Y., Fong D.Y.T., Ho J.C.Y., Ching C.K. and Chen J.S., Helicobacter pylori Eradication to Prevent Gastric Cancer in a High-Risk Region of China: a Randomized Controlled Trial, The Journal of American Medical Association. 2004, 291(2): 187-194.

Wong B.C.Y., Lam S.K., Wong R.W.M., Chen J.S., Zheng T., Feng R., Lai K.C., Hu H.C., Yuen S.T., Leung S.Y., Fong D.Y.T., Ho J.W.C. and Ching C.K., Helicobacter pylori eradication to prevent gastric cancer in a high-risk region in China: A randomized controlled trial, JAMA. 2004, 291(2): 187-194.

Zhao W., Chan T.L., Chu K.M., Chan A.S.Y., Stratton M.R., Yuen S.T. and Leung S.Y., Mutations of BRAF and KRAS in gastric cancer and their association with microsatellite instability, International Journal of Cancer. 2004, 108(1): 167-169.

Researcher : Li YH

List of Research Outputs

Ma E.S.K., Kwok J.S.Y., Li Y.H. and Chan L.C., Clinical significance of ADAMTS13 mutational analysis in relapsing TTP with severe vWF-cleaving protease deficiency, Blood. 2003, 102(11) Part 1: 802a.

Ma E.S.K., Kwok J.S.Y., Li Y.H. and Chan L.C., Clinical significance of ADAMTS13 mutational analysis in relapsing TTP with severe vWF-cleaving protease deficiency, Proceedings of the American Society of Hematology, San Diego, USA, 6-9 December 2003. Abstract No. 2970.

Researcher : Liang ACT

List of Research Outputs

Chen Y.W., Liang A.C.T., Au W.Y., Chu K.M., Wong K.Y., Hu X., Lu L., Tang J.C.O., Chan K.W., Beh S.L., Kwong Y.L., Liang R.H.S. and Srivastava G., Multiple BCL6 translocation partners in individual cases of gastric lymphoma, Blood. 2003, 102: 1931-1932.

Law B.F.F., Srivastava G., Wong K.Y., Chen W.Y.W., Liang A.C.T., Lam K.Y., Law S.Y.K., Wong J. and Tang J.C.O., A novel tumor transforming gene at 7q22, GAESCC1, is frequently amplified and overexpressed in primary esophageal squamous cell carcinomas, Abstract of the Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Shen L., Liang A.C.T., Lu L., Au W.Y., Wong K.Y., Tin P.C., Chan K.W., Ko K.H., Chen W.Y.W., Beh S.L., Shimizu N., Tsuchiyama T., Tang J.C.O., Kwong Y.L., Liang R.H.S. and Srivastava G., Aberrant BCL10 nuclear expression in nasal NK/T-cell lymphoma, Blood. 2003, 102(4): 1553-1554.

Shen L., Liang A.C.T., Au W.Y., Lu L., Chen W.Y.W., Wong K.Y., Tang J.C.O., Chan K.W., Beh S.L., Kwong Y.L., Liang R.H.S. and Srivastava G., BCL10 mutations are irrelevant to its aberrant nuclear localization in nasal NK/T-cell lymphoma, Leukemia. 2003, 17(11): 2240-2242.

Researcher : Liu W

List of Research Outputs

Khoo U.S., Chan Y.K., Yip S.P., Peiris M., Tin V., Liu W., Xue W., Ngan H.Y.S., Chan L.C. and Cheung A.N.Y., The tandem repeat polymorphism of the DC-SIGNR gene is associated with susceptibility to SARS coronavirus infection in Chinese from Hong Kong, Proceedings of the Human Genome Organization (HUGO) Meeting, April 4-7, 2004, Berlin, Germany. 2004.

Researcher : Lo CK

List of Research Outputs

Huang F., Ma L., Wu A.Y.Y., Tian L., Chan K.W., Chan A.K., Lam A.C., Lo C.K., Ko K.H., To K.W., Chik S., Kam S.K., Fong I., Li X.S., Ng M.H. and MacPherson G.G., Systemic autoimmune disease induced by dendritic cells that have captured dying cells, Proceedings of the Frontiers in Biomedical Research, Hong Kong. 12 December 2003.

Ma L., Chan A.K., Lam A.C., Chan K.W., Lo C.K., Ko K.H., To K.W., Wu A.Y.Y., Tian L., Ng M.H. and Huang F., The role of dendritic cells in the induction of systemic autoimmune disease, Proceedings of the 8th Research Postgraduate Symposium, Hong Kong, 13 December 2003.

Researcher : Loong F

List of Research Outputs

Chim J.C.S., Hu W.H., Loong F., Ooi C.G.C. and Liang R.H.S., Gastrointestinal manifestations of mantle cell lymphoma, Gastrointestinal Endoscopy. 2003, 59: 931-933.

Chim J.C.S., Loong F. and Chu K.M., MALT lymphoma of small bowel: an elusive cause of recurrent upper GIB, Leuk lymphoma. 2004, 45(2): 405-7.

Chim J.C.S., Loong F., Ooi C.G.C. and Liang R.H.S., Mantle cell lymphoma of prostate gland, Journal of Clinical Oncology. 2003, 21: 4456-4458.

Chim J.C.S., Wong K.Y., Loong F. and Srivastava G., SOCS1 and SHP1 hypermethylation in mantle cell lymphoma and follicular lymphoma: implications for epigenetic activation of the Jak/STAT pathway, Leukemia. 2004, 18(2): 356-372.

Chim J.C.S., Ooi C.G.C., Loong F. and Liang R.H.S., Solitary bone plasmacytoma : Role of MRI and PET, Journal of Clinical Oncology. 2004, 22: 1328-1330.

Researcher : Lu L

Project Title:	The regulatory role of tumor suppressor gene Pten in B cell development and neoplasia
Investigator(s):	Dr. Lu L.W., Prof. Wu G.E.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2001
Completion Date:	10/2003

Abstract:

To examine the role of Pten in regulating B cell growth and survival; to examine the effect of Pten insufficiency on B cell development and neoplasia in Pten +/- mice

Project Title:	Pathogenic effects of SARS-associated coronavirus infection on lymphoid system
Investigator(s):	Dr. Lu L.W., Prof. Chan L.C., Dr. Jones B.M., Dr. Loong F., Dr. Ma E.S.K., Prof. Peiris J.S.M., Dr. Zheng B.
Department:	Pathology
Source(s) of Funding:	SARS Research Fund
Start Date:	07/2003

Abstract:

To examine the pathological changes of lymphoid organs and the distribution of SARS-CoV infected cells from patients with SARS; to investigate the SARS-CoV trophism in immune cells and characterize the phenotypic and functional changes of viral-infected lymphocytes.

Project Title:	B cell apoptosis and its regulation in autoimmunity
Investigator(s):	Dr. Lu L.W., Prof. Kincade P.W.
Department:	Pathology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	09/2003

Abstract:

To study the regulation of B cell development and survival by BLyS and APRIL in mouse bone marrow; to examine B cell apoptosis and its regulation in the development of CIA as a model of RA; to determine if BLyS and/or APRIL participate(s) in the pathogenesis of CIA.

Project Title:	B cell apoptosis and its regulation in autoimmunity
Investigator(s):	Dr. Lu L.W., Prof. Kincade P.W.
Department:	Pathology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	09/2003

Abstract:

Project Title:	Specific gene-silencing therapy for rheumatoid arthritis in collagen-induced mice
Investigator(s):	Dr. Lu L.W., Mr. Lam D., Dr. Lin M.C.M.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	09/2003
Completion Date:	08/2004

Abstract:

To construct si-RNA of BLyS and the adenovirus-associated vectors carrying the si-RNA of BLyS (AAV-siRNA-BLyS) to silence BLyS gene expression in primary immune cells; to examine the gene-silencing effect of AAV-siRNA-BLyS on the activation and function of lymphocytes from normal and CIA mice.

Project Title:	Effect of aging on B lymphopoiesis
Investigator(s):	Dr. Lu L.W.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2003

Abstract:

To determine if B cell differentiation and maturation are impaired during aging process; to identify the differentiation stages at which developing B cells are susceptible to the influence of aging; to study the mechanisms underlying the change of B lymphopoiesis associated with aging.

List of Research Outputs

Chen Y.W., Liang A.C.T., Au W.Y., Chu K.M., Wong K.Y., Hu X., Lu L., Tang J.C.O., Chan K.W., Beh S.L., Kwong Y.L., Liang R.H.S. and Srivastava G., Multiple BCL6 translocation partners in individual cases of gastric lymphoma, Blood. 2003, 102: 1931-1932.

Shen L., Liang A.C.T., Lu L., Au W.Y., Wong K.Y., Tin P.C., Chan K.W., Ko K.H., Chen W.Y.W., Beh S.L., Shimizu N., Tsuchiyama T., Tang J.C.O., Kwong Y.L., Liang R.H.S. and Srivastava G., Aberrant BCL10 nuclear expression in nasal NK/T-cell lymphoma, Blood. 2003, 102(4): 1553-1554.

Shen L., Liang A.C.T., Au W.Y., Lu L., Chen W.Y.W., Wong K.Y., Tang J.C.O., Chan K.W., Beh S.L., Kwong Y.L., Liang R.H.S. and Srivastava G., BCL10 mutations are irrelevant to its aberrant nuclear localization in nasal NK/T-cell lymphoma, Leukemia. 2003, 17(11): 2240-2242.

Tian L., Lu L., Yuan Z.W., Lam J.R. and Tam P.K.H., Acceleration of apoptosis in CD4+CD8+ thymocytes by rapamycin accompanied by increased CD4+CD25+ T cells in the periphery, Transplantation. 2004, 77(2): 183-189.

Woo M., Hakem R., Furlonger C., Hakem A., Duncan G.S., Sasaki T., Bouchard D., Lu L., Wu G.E., Paige C.J. and Mak T.W., Caspase-3 regulates cell cycle in B cells: a consequence of substrate specificity, Nature Immunology. 2003, 4(10): 1016-1022.

Xiao D.S., Jiang L., Che L. and Lu L., Nitric oxide and iron metabolism in exercised rat with L-arginine supplementation, Molecular & Cellular Biochemistry. 2003, 252(1-2): 65-72.

Zhang M., Srivastava G. and Lu L., The pre-B cell receptor and its function during B cell development, Cellular and Molecular Immunology. 2004, 1(2): 89-94.

Zheng B., Guan Y., Wong K.H., Zhou J., Wong K.L., Young B.W.Y., Lu L. and Lee S.S., SARS-related virus predating SARS outbreak, Hong Kong, Emerging Infectious Diseases. 2004, 10(2): 176-178.

Researcher : Ma ESK

Project Title:	Diagnosis of a-thalassaemia mutations in the Chinese by single tube multiplex polymerase chain reaction
Investigator(s):	Dr. Ma E.S.K.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2002
Completion Date:	10/2003

Abstract:

To develop a single tube multiplex polymerase chain reaction (PCR) for the detection of five commonest a-globin gene mutations in the Chinese, namely (--^SEA) a-thalassaemia deletion (SEA deletion), single a-globin gene deletion of the rightward (--a^3.7) or leftward (-a^4.2) type, Hb Constant Spring and Hb Quong Sze.

Project Title:	Genetic modifiers of b-thalassaemia phenotype in the Chinese
Investigator(s):	Dr. Ma E.S.K., Dr. Tan-Un K.C.
Department:	Pathology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	12/2002

Abstract:

The project is to investigate genetic modifiers of b-thalassaemia phenotype in Chinese patients at candidate gene loci responsible for g-globin chain production, iron metabolism, bilirubin metabolism and collagen synthesis. The information gathered is applicable not only to genetic counselling and prenatal diagnosis, but will also provide prognostic indicators on which management decisions can be based.

Project Title:	Quantification of mutant transcripts in mild and silent b-thalassaemia alleles of the Chinese by real time polymerase chain reaction
Investigator(s):	Dr. Ma E.S.K.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2003

Abstract:

To determine the level of mutant transcripts in these mild and silent b-thalassaemia alleles by real time polymerase chain reaction.

List of Research Outputs

Au W.Y., Ooi C.G.C., Yau W.F., Ma E.S.K., Wan T.S.K. and Kwong Y.L., Chronic myeloid leukemia in an adolescent with Ollier’s diease after intensive X ray exposure, Leukemia and Lymphoma. 2004, 45: 613-616.

Au W.Y., Tsang J., Cheng T.S..., Chow W.S., Woo Y.C., Ma E.S.K. and Tam S.C.F., Cough mixture abuse as a novel cause of megaloblastic anemia and peripheral neuropathy, British Journal of Haematology. 2003, 123: 956-958.

Au W.Y., Fung A., Chim J.C.S., Lie A.K.W., Liang R.H.S., Ma E.S.K., Chan C.H., Wong K.F. and Kwong Y.L., FLT-3 aberrations in acute promyelocytic leukaemia: clinicopathological associations and prognostic impact, British Journal of Haematology. 2004, 125(4): 463-469.

Au W.Y., Lie A.K.W., Ma E.S.K., Lau G., Chan C. and Kwong Y.L., Late onset pure red cell aplasia due to delayed lymphoid engraftment complicating ABO mismatched hematopoietic stem cell transplantaion, Transfusion. 2004, 44: 946-947.

Au W.Y., Fung T.K., Ma E.S.K., Liang R.H.S. and Kwong Y.L., Low frequency of FLT3 gene internal tandem duplication and activating loop mutation in therapy-related acute myelocyticleukemia and myelodysplastic syndrome, Cancer Genetics and Cytogenetics. 2004, 149(2): 169-172.

Au W.Y., Ma E.S.K., Wan T.S.K., Man C.W.Y. and Kwong Y.L., Pentasomy 8q after cyclophosphamide therapy for fibrosing alveolitis: case report and review, Cancer Genetics and Cytogenetics. 2003, 141: 79-82.

Au W.Y., Lie A.K.W., Chan C., Pang A.W.K., Liang R.H.S., Ma E.S.K., Choy C.K.W. and Kwong Y.L., Treatment of post-renal transplantation lymphoproliferative disease manifesting as plasmacytoma with non-myeloablative hematopoietic stem cell transplantation from the same kidney donor, American Journal of Hematology. 2003, 74: 283-6.

Chan S., Huang F., Chim J.C.S., Ma E.S.K., Law H.K.W., Lau Y.L. and Chan G.C.F., Dendritic cells (DBS) can be induced from marrows of both newly diagnosed lymphoid and myeloid leukaemia but they demonstrated different morphological and immunological behaviours, Joint Annual Scientific Meeting 2003 of the Hong Kong Paediatric Society and Hong Kong Paediatric Nurses Association Limited, QEH, Hong Kong, 13 December 2003. 119.

Chim J.C.S., Ma E.S.K. and Ooi C.G.C., Magnetic Resonance (MR) Imaging of Leptomeningeal Lymphoma, British Journal of Haematology. 2004, 124(5): 566.

Chiu R.W.K., Ding C., Lau T.K., Leung T.N., Chan L.C., Chan A.Y.Y., Charoenkwan P., Ng I.S.L., Xu X.M., Wanapirak C., Sanguansermsri T., Liao C., Ma E.S.K., Chui D.H.K., Cantor C.R. and Lo Y.M.D., Non-invasive prenatal diagnosis of -thalassaemia by mass spectrometric analysis of fetal DNA in maternal plasma, Proceedings of the 9th International Conference on Thalassaemia and Haemoglobinopathies. Citt? del Mare, Terrasini, Palermo, Italy, 15–19 October 2003.

Hon C., Chan G.C.F., Ha S.Y., Ma E.S.K., Wong K.F. and Au W.Y., Bone marrow transplantation for therapy-related acute myeloid leukemia in congenital retinoblastoma associated with 13q deletion syndrome, Annals of Hematology. Springer-Verlag, 2004, 83: 481-483.

Jones B.M., Ma E.S.K., Peiris J.S.M., Wong P.C., Ho J.C.M., Lam B., Lai K.N. and Tsang K.W.T., Prolonged disturbances of in vitro cytokine production in patients with severe acute respiratory syndrome (SARS) treated with ribavirin and steroids, Clinical & Experimental Immunology. 2004, 135: 467-473.

Lee C.K., Ma E.S.K., Shek T.W.H., Lam C.C.K., Au W.Y., Wan T.S.K. and Chan L.C., Plasmablastic transformation of multiple myeloma, Human Pathology. 2003, 34(7): 710-714.

Lee C.K., Ma E.S.K., Tang M., Lam C.C.K., Lin C.K. and Chan L.C., Prevalence and specificity of clinically significant red cell alloantibodies in Chinese women during pregnancy - a review of cases from 1997 to 2001, Transfusion Medicine. 2003, 13(4): 227.

Leung K.F.S. and Ma E.S.K., Amyloid deposits in the bone marrow, British Journal of Haematology. 2003, 121(5): 679.

Leung K.F.S., Ma E.S.K., Chan A.Y.Y. and Chan L.C., Clinical phenotype of haemoglobin Q-H disease, Journal of Clinical Pathology. 2004, 57: 81-82.

Leung K.Y., Lee C.P., Tang M.H.Y., Chan H.Y. and Ma E.S.K., Detection of raised cerebral blood flow and cardiomegaly in fetus affected by HbH disease: two case reports, Proceedings of the 13th World Congress on Ultrasound in Obstetrics and Gynecology, Paris, France, 31 August – 4 September 2003.

Leung K.Y., Lee C.P., Tang M.H.Y., Chan H.Y. and Ma E.S.K., Detection of raised cerebral blood flow and cardiomegaly in fetuses affected by Hb H disease : two case reports, 13th World Congress on Ultrasound in Obstetrics and Gynaecology, August 31-September 4, 2003, Paris, France. 2003.

Ma E.S.K., Kwok J.S.Y., Li Y.H. and Chan L.C., Clinical significance of ADAMTS13 mutational analysis in relapsing TTP with severe vWF-cleaving protease deficiency, Blood. 2003, 102(11) Part 1: 802a.

Ma E.S.K., Kwok J.S.Y., Li Y.H. and Chan L.C., Clinical significance of ADAMTS13 mutational analysis in relapsing TTP with severe vWF-cleaving protease deficiency, Proceedings of the American Society of Hematology, San Diego, USA, 6-9 December 2003. Abstract No. 2970.

Ma E.S.K., Lam K.K.Y. and Tan-Un K.C., Genetic haemochromatosis and iron overload in Chinese patients with b-thalassaemia, Proceedings of the 9th International Conference on Thalassaemia and Haemoglobinopathies. Citt? del Mare, Terrasini, Palermo, Italy, 15–19 October 2003.

Ma E.S.K., Au W.Y., Lie A.K.W., Ng I.O.L., Leung A.Y.H., Tse E.W.C., Liang R.H.S., Lau G. and Kwong Y.L., Liver graft-versus-host disease after donor lymphocyte infusion for relapses of hematologic malignancies post allogeneic hematopoietic stem cell transplantation, Bone Marrow Transplantation. 2004, 34: 57-61.

Wan T.S.K., Ma E.S.K., Li C.K. and Chan L.C., Atypical fluorescence in situ hybridisation pattern in chronic myeloid leukaemia due to cryptic insertion of BCR at 9q34, Leukemia. 2004, 18(1): 161-162.

Wan T.S.K., Ma E.S.K., Lam C.C.K., Chan L.C., Lee K.K. and Au W.Y., Deletion 9q as the sole karyotypic abnormality in myelocytic disorders: a new case of myelodysplastic syndrome and its prognostic implications in acute myelocytic leukemia, Cancer Genetics and Cytogenetics. 2003, 145(2): 184-186.

Wang W., Tan A.S.-.C., Kham S.K.Y., Chan A.Y.Y., Prior J., Erber W.N., Ma E.S.K., Chan L.C., Chui D.H.K., Quah T.C. and Chong S.S., Improved tools for molecular diagnosis of a- and b-thalassemia in Southeast Asia, Proceedings of the 9th International Conference on Thalassaemia and Haemoglobinopathies. Citt? del Mare, Terrasini, Palermo, Italy, 15-19 October 2003.

Wang W., Ma E.S.K., Chan A.Y.Y., Prior J., Erber W.N., Chan L.C., Chui D.H.K. and Chong S.S., Single-tube multiplex-PCR screen for anti-3.7 and anti-4.2 a-globin gene triplications, Clinical Chemistry. 2003, 49(10): 1679-1682.

Wong W.S., Chan A.Y.Y., Yip S.F. and Ma E.S.K., Thalassemia intermedia due to co-inheritance of b⁰/b⁺-thalassemia and (--^SEA) a-thalassemia/Hb Westmead [a122(H5)His > Gln (a2)] in a Chinese family, Hemoglobin. 2004, 28(2): 151-156.

Researcher : Ma L

List of Research Outputs

Huang F., Ma L., Wu A.Y.Y., Tian L., Chan K.W., Chan A.K., Lam A.C., Lo C.K., Ko K.H., To K.W., Chik S., Kam S.K., Fong I., Li X.S., Ng M.H. and MacPherson G.G., Systemic autoimmune disease induced by dendritic cells that have captured dying cells, Proceedings of the Frontiers in Biomedical Research, Hong Kong. 12 December 2003.

Ma L., Chan A.K., Lam A.C., Chan K.W., Lo C.K., Ko K.H., To K.W., Wu A.Y.Y., Tian L., Ng M.H. and Huang F., The role of dendritic cells in the induction of systemic autoimmune disease, Proceedings of the 8th Research Postgraduate Symposium, Hong Kong, 13 December 2003.

Researcher : Nanji AA

List of Research Outputs

Tipoe G.L., Leung T.M., Liong E.C., So H., Leung K.M., Tom W.M., Fan S.T. and Nanji A.A., Nitric oxide in an acute liver injury model (Abstract), The 5th Annual Scientific Meeting, Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 3 January 2004.

Researcher : Ng IOL

Project Title:	Identification and characterization of DLC-2, a candidate tumour suppressor gene on 13q frequently deleted in liver cancer
Investigator(s):	Prof. Ng I.O.L., Dr. Jin D.Y.
Department:	Pathology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	09/2001

Abstract:

To clone the full-length cDNA and genomic sequences of human DLC-2; to determine the accurate chromosomal localizatioin of DLC-2; to analyze the expression pattern of DLC-2 mRNA and protein in tissues and cells; to define the genotypic and phenotypic alterations of DLC-2 in HCC and hepatoma cell lines; to test the RhoGAP and tumour supressor activities of DLC-2; to characterize the signaling pathways as related to DLC-2 and cell transformation; to assess the clinical and pathological significance of DLC-2 in human HCC.

Project Title:	Dysregulation of NF-kB signaling in liver cancer
Investigator(s):	Prof. Ng I.O.L., Dr. Jin D.Y., Dr. Yau T.O.
Department:	Pathology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	09/2002

Abstract:

The project attempts to document systematically the dyregulation of the important cellular pathway in liver cancer and identify its clinical significance in patient management.

Project Title:	Chimerism in transplanted liver
Investigator(s):	Prof. Ng I.O.L.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2002

Abstract:

To evaluate the chimerism in the transplanted livers in patients after liver transplantation; to delineate cell dynamics, viz. differentiation (into hepatocytes or other cells) of recipient cells, i.e. of extrahepatic origin, in the transplanted liver grafts.

Project Title:	Functional characterization of novel genes in liver cancer
Investigator(s):	Prof. Ng I.O.L., Prof. Gu J.R., Prof. Wan D.F.
Department:	Pathology
Source(s) of Funding:	National Natural Science Foundation of China
Start Date:	01/2003

Abstract:

To define the genetic and phenotypic alterations of the 3 novel genes (LASS2, PP753 and SP192) in human HCC samples and HCC cell lines; to assess the tumour suppressor functions and the signaling pathways of these genes in HCC cells; to evaluate the clinicopathological and prognostic significance of the genes in human HCC.

Project Title:	Study on the pathogenesis of SARS coronavirus by establishing transgenic mouse lines expressing SARS-CoV viral proteins
Investigator(s):	Prof. Ng I.O.L., Dr. Chan D.W., Dr. Huang F.P., Dr. Jin D.Y., Prof. Peiris J.S.M., Dr. Poon L.L.M., Dr. Sham M.H., Dr. Yau T.O.
Department:	Pathology
Source(s) of Funding:	SARS Research Fund
Start Date:	07/2003

Abstract:

To develop transgenic mouse lines that can express the viral proteins of the SARS-coronavirus (CoV) in an inducible form, in tissue-specific and in tissue-nonspecific manner.

Project Title:	Deciphering dysregulation of mitotic checkpoint control in liver cancer
Investigator(s):	Prof. Ng I.O.L., Dr. Jin D.Y.
Department:	Pathology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	09/2003

Abstract:

1) To document and characterize dysregulation of mitotic checkpoint in hepatocellular carcinoma (HCC) by evaluating; a) mitotic checkpoint competence in HCC cell lines, b) expression and alterations of mitotic checkpoint genes and proteins in HCC cells and tissues, c) epigenetic causes of mitotic checkpoint defects in HCC; 2) to investigate the roles of hepatitis B virus-encoded X protein (HBx) in mitotic checkpoint in HCC; 3) to assess the clinical, pathological and prognostic significance of mitotic checkpoint dysfunction in HCC.

Project Title:	Deciphering dysregulation of mitotic checkpoint control in liver cancer
Investigator(s):	Prof. Ng I.O.L., Dr. Jin D.Y.
Department:	Pathology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	09/2003

Abstract:

Project Title:	Characterization of T-cadherin in hepatocellular carcinoma
Investigator(s):	Prof. Ng I.O.L., Dr. Chan D.W.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2003

Abstract:

To evaluate the expression of T-cadherin at mRNA and protein levels in human HCCs and in HCC cell lines; to investigate the factors which induce expression of T-cadherin in HCC.

List of Research Outputs

Chan C.F., Yau T.O., Jin D., Wong C.M., Fan S.T. and Ng I.O.L., Evaluation of nuclear factor-kB, urokinase-type plasminogen activator, and HBx and their clinicopathological significance in hepatocellular carcinoma, Clinical Cancer Research. 2004, 10(12 pt. 1): 4140-4149.

Chan D.W., Lee M.F. and Ng I.O.L., Characterization of H-cadherin expression in human hepatocellular carcinoma, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Chan D.W. and Ng I.O.L., HBV X gene is required for maintaining the tumorigenicity of hepatocellular carcinoma cells, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Chan O.O., Ng I.O.L., Lam C.M., Shek T.W.H. and Lai C.L., Cholestatic jaundice caused by sequential carbimazole and propylthiouracil treatment for thyrotoxicosis, Hong Kong Medical Journal. 2003, 9(5): 377-380.

Cheung S.T., Chen X., Fong D.Y.T., Leung K.L., Ip Y.C., Ng I.O.L., So S. and Fan S.T., Delineation of prognostic biomarkers from the global gene expression profile of liver cancer (Abstract), The 5th Annual Scientific Meeting, Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 3 January 2004.

Cheung S.T., Chen X., Leung K.L., So S., Ng I.O.L. and Fan S.T., Different gene expression profiles in cirrhotic livers from cancer patients compared to non-cancer patients (Abstract), Proceedings of the American Association for Cancer Research. 2003, 44: 2500.

Cheung S.T., Chen X., Leung K.L., So S., Ng I.O.L. and Fan S.T., Different gene expression profiles in cirrhotic livers from cancer patients compared to non-cancer patients (Abstract), The 94th Annual Meeting of the American Association for Cancer Research, Washington, D.C., U.S.A., 11-14 July 2003.

Cheung S.T., Chen X., Fong D.Y.K., Leung K.L., Ip Y.C., Ng I.O.L. and Fan S.T., Novel prognostic markers for hepatocellular carcinoma (Poster Presentation), The Spanish National Cancer Centre 2004 Symposium on the Molecular Taxonomy of Cancer, Madrid, Spain, 3-6 February 2004.

Cheung S.T., Chen X., Fong D.Y.K., Leung K.L., Ip Y.C., Ng I.O.L., So S. and Fan S.T., Novel prognostic markers for liver cancer (Abstract), Frontiers in Biomedical Research, Faculty of Medicine, The University of Hong Kong, Hong Kong, 12 December 2003.

Ching Y.P., Leong V.Y.L., Jin D. and Ng I.O.L., Regulation of the tumour metastasis suppressor gene, NM23, by p21-activated protein kinase 5., Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Ho M.K.S., Lee M.F., Chan C.K.L. and Ng I.O.L., Allelic alterations in nontumorous liver tissues and corresponding hepatocellular carcinomas from Chinese patients, Human Pathology. 2003, 34(7): 699-705.

Lam C.M., Chan O.O., Ho P., Ng I.O.L., Lo C.M., Liu C.L., Poon R.T.P. and Fan S.T., Different presentation of hepatitis B-related hepatocellular carcinoma in a cohort of 1863 young and old patients - implications for screening, Alimentary Pharmacology and Therapeutics. 2004, 19(7): 771-777.

Lee K.W., Man K., Ho J.W.Y., Sun K.W., Ng T.P., Ng I.O.L., Xu R. and Fan S.T., FTY720 selectively induces human hepatoma cell lines apoptosis via Akt dephosphorylation (Abstract), The 5th Annual Scientific Meeting, Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 3 January 2004.

Lee K.W., Man K., Ling M.T., Wang X., Wong Y.C., Lo C.M., Poon R.T.P., Ng I.O.L. and Fan S.T., Over-expression of Id-1 induces cell proliferation in hepatocellular carcinoma through inactivation of p16^INK4a/RB pathway, Carcinogenesis. 2003, 24(11): 1729-1736.

Lee P.K.M., Samman N. and Ng I.O.L., Unicystic ameloblastoma--use of Carnoy's solution after enucleation, International Journal of Oral and Maxillofacial Surgery . 2004, 33(3): 263-267.

Leung H.Y., Ching Y.P., Wong C.M., Ng D.C.H., Jin D. and Ng I.O.L., Characterization of novel putative tumor suppressor gene, DLC2 (deleted in liver cancer 2), in hepatocellular carcinoma, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Liu C.L., Fan S.T., Lo C.M., Wong Y., Ng I.O.L., Lam C.M., Poon R.T.P. and Wong J., Abdominal drainage after hepatic resection is contraindicated in patients with chronic liver diseases, Annals of Surgery. 2004, 239(2): 194-201.

Liu C.L., Fan S.T., Lo C.M., Chan S.C., Ng I.O.L., Tso W.K. and Wong J., Hepatic resection for incidentaloma (Poster of Distinction), Digestive Disease Week, New Orleans, U.S.A., 15-20 May 2004.

Liu C.L., Fan S.T., Lo C.M., Chan S.C., Ng I.O.L., Tso W.K. and Wong J., Heptic resection for incidentaloma (Abstract), The 5th Annual Scientific Meeting, Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 3 January 2004.

Liu C.L., Fan S.T., Lo C.M., Chan S.C., Ng I.O.L., Lai C.L. and Wong J., Interleukin-2 receptor antibody (basiliximab) for immunosuppressive induction therapy after liver transplantation: a protocol with early elimination of steroids and reduction of tacrolimus dosage, Liver Transplantation. 2004, 10(6): 728-733.

Lo C.M., Cheung S.T., Ng I.O.L., Liu C.L., Lai C.L. and Fan S.T., Fibrosing cholestatic hepatitis secondary to precore/core promoter hepatitis B variant with lamivudine resistance: successful retransplantation with combination adefovir dipivoxil and hepatitis B immunoglobulin, Liver Transplantation. 2004, 10(4): 557-563.

Lo C.M., Fan S.T., Liu C.L., Yong B.H., Wong Y., Ng I.O.L. and Wong J., Safety and outcome of hepatitis B core antibody-positive donors in right-lobe living donor liver transplantation, Liver Transplantation. 2003, 9(8): 827-832.

Ma E.S.K., Au W.Y., Lie A.K.W., Ng I.O.L., Leung A.Y.H., Tse E.W.C., Liang R.H.S., Lau G. and Kwong Y.L., Liver graft-versus-host disease after donor lymphocyte infusion for relapses of hematologic malignancies post allogeneic hematopoietic stem cell transplantation, Bone Marrow Transplantation. 2004, 34: 57-61.

Ma S.Y., Au W.Y., Ng I.O.L., Lie A.K.W., Liang R.H.S., Lau G. and Kwong Y.L., Hepatitic graft-versus-host disease after hematopoietic stem cell transplantation : clinicopathologic features and prognostic implication, Transplantation. 2004, 77: 1252-1259.

Ma S.Y., Au W.Y., Ng I.O.L., Lie A.K., Leung A.Y., Liang R.H.S., Lau G. and Kwong Y.L., Role of liver biopsy in the management of liver dysfunction after hematopoietic stem-cell transplantation in a hepatitis B virus-prevalent patient population, Transplantation. 2003, 7691): 169-176.

Ng I.O.L., Chan K.L., Shek T.W.H., Lee M.F., Fong D.Y.T., Lo C.M. and Fan S.T., High frequency of chimerism in transplanted livers (Abstract), The 5th Annual Scientific Meeting, Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 3 January 2004.

Ng I.O.L., Chan K.L., Shek T.W.H., Lee J.M., Fong D.Y.T., Lo C.M. and Fan S.T., High frequency of chimerism in transplanted livers, Hepatology. 2003, 38(4): 989-998.

Ng K.K.C., Poon R.T.P., Lo C.M., Liu C.L., Lam C.M., Ng I.O.L. and Fan S.T., Impact of preoperative fine-needle aspiration cytologic examination on clinical outcome in patients with hepatocellular carcinoma in a tertiary referral center, Archives of Surgery. 2004, 139(2): 193-200.

Ooi C.G.C., Khong P.L., Chan G.C.F., Chan K.N., Chan K.L., Lam W.W.M., Ng I.O.L. and Ha S.Y., Magnetic resonance screening of iron status in transfusion-dependent b-thalassaemia patients, British Journal of Haematology. Blackwell Publishing Ltd, 2004, 124: 385-390.

Pang A., Ng I.O.L., Fan S.T. and Kwong Y.L., Clinicopathologic significance of genetic alterations in hepatocellular carcinoma, Cancer Genetics and Cytogenetics. 2003, 146(1): 8-15.

Pang R., Yuen J., Yuen M.F., Lai C.L., Lee K.W., Man K., Poon R.T.P., Fan S.T., Wong C.M., Ng I.O.L., Kwong Y.L. and Tse E., PIN1 over-expression and b-catenin gene mutations are distinct oncologic events in human hepatocarcinogenesis (Oral Presentation), The 8th Research Postgraduate Symposium, The University of Hong Kong, Hong Kong, 13 December 2003.

Pang R.W.C., Yuen J.C.H., Yuen M.F., Lai C.L., Lee K.W., Man K., Poon R.T.P., Fan S.T., Wong C.M., Ng I.O.L., Kwong Y.L. and Tse E.W.C., PIN1 over-expression and β-catenin gene mutations are distinct oncogenic events in human hepatocellular carcinoma, Oncogene. 2004, 23(23): 4182-6.

Pawlik T.M., Poon R.T.P., Abdalla E., Ikai I., Curley S., Nagorney D., Belghiti J., Ng I.O.L., Yamaoka Y., Lauwers G. and Vauthey J.N., Hepatectomy for hepatocellular carcinoma with major portal or hepatic vein invasion: results of a multicenter study (Oral Presentation), The 6th World Congress of the International Hepato-Pancreato-Biliary Association, Washington, D.C., U.S.A., 2-6 June 2004.

Pawlik T.M., Poon R.T.P., Abdalla E., Ikai I., Curley S., Nagorney D., Belghiti J., Ng I.O.L., Yamaoka Y., Lauwers G. and Vauthey J.N., Hepatitis serology defines tumor and liver disease characteristics but not prognosis after resection of hepatocellular carcinoma (Oral Presentation), The 45th Annual Scientific Meeting of the Society for Surgery of Alimentary Tract, New Orleans, U.S.A., 17 May 2004.

Ren Y., Tsui H.T., Poon R.T.P., Ng I.O.L., Li Z., Chen Y., Jiang G., Lau C.P.Y., Yu W.C., Bacher M. and Fan S.T., Macrophage migration inhibitory factor: roles in regulating tumor cell migration and expression of angiogenic factors in hepatocellular carcinoma, International Journal of Cancer. 2003, 107(1): 22-29.

Sze M.F., Ching Y.P., Jin D. and Ng I.O.L., Dysregulation of mitotic spindle checkpoint control in hepatocellular carcinomas, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Tong A.C.K., Ng I.O.L. and Yan B.S.W., Variations in clinical presentations of the simple bone cyst: report of cases, Journal of Oral and Maxillofacial Surgery. 2003, 61(12): 1487-1491.

Tsang W.M., Tong A.C.K., Chow L.T.C. and Ng I.O.L., Massive osteolysis (Gorham disease) of the maxillofacial skeleton: report of 2 cases, Journal of Oral and Maxillofacial Surgery. 2004, 62(2): 225-230.

Wong B.W., Luk J.M.C., Ng I.O.L., Hu M.Y., Liu K.D. and Fan S.T., Identification of liver-intestine cadherin in hepatocellular carcinoma--a potential disease marker, Biochemical and Biophysical Research Communications. 2003, 311(3): 618-624.

Wong C.M., Ching Y.P., Lee M.F., Jin D. and Ng I.O.L., Genetic and epigenetic alterations of DLC-1 gene in hepatocellular carcinoma, Cancer Research. 2003, 63: 7646-7651.

Yuen M.F., Kato T., Mizokami M., Chan O.O., Yuen J.C.H., Yuan H., Wong D.K.H., Sum S.M., Ng I.O.L., Fan S.T. and Lai C.L., Clinical outcome and virologic profiles of severe hepatitis B exacerbation due to YMDD mutations, Journal of Hepatology. 2003, 39(5): 850-855.

Yuen M.F., Wong D.K.H., Sablon E., Tse E.W.C., Ng I.O.L., Yuan H., Siu C.W., Sander T.J., Bourne E.J., Hall J.G., Condreay L.D. and Lai C.L., HBsAg seroclearance in chronic hepatitis B in the Chinese: Virological, histological, and clinical aspects, Hepatology. 2004, 39(6): 1694-1701.

Researcher : Ng KM

List of Research Outputs

Cheung A.N.Y., Szeto E.F., Ng K.M., Fong K.W., Ang Chan E.Y., Tsun O.K.L., Khoo U.S., Chan Y.K. and Ng W.Y., Atypical squamous cells of undetermined significance on cervical smears: follow-up study of an Asian screening population, Cancer Cytopathology. 2004, 102(2): 74-80.

Researcher : Ng WY

List of Research Outputs

Cheung A.N.Y., Szeto E.F., Ng K.M., Fong K.W., Ang Chan E.Y., Tsun O.K.L., Khoo U.S., Chan Y.K. and Ng W.Y., Atypical squamous cells of undetermined significance on cervical smears: follow-up study of an Asian screening population, Cancer Cytopathology. 2004, 102(2): 74-80.

Cheung A.N.Y., Szeto E.F., Leung B.S.Y., Khoo U.S. and Ng W.Y., Liquid-based cytology and conventional cervical smears: a comparison study in an Asian screening population, Cancer. 2003, 99(6): 331-335.

Tam K.F., Cheung A.N.Y., Liu K.L., Ng T.Y., Pun T.C., Chan Y.M., Wong R.L.C., Ng W.Y. and Ngan H.Y.S., A retrospective review on atypical glandular cells of undetermined significance (AGUS) using the Bethesda 2001 classification, Gynecologic Oncology. 2003, 91(3): 603-607.

Researcher : Nicholls JM

Project Title:	Analysis of 14-3-3 protein and methylation status in untreated patients with nasopharyngeal carcinoma (NPC)
Investigator(s):	Dr. Nicholls J.M.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2002
Completion Date:	10/2003

Abstract:

Nasopharyngeal carcinoma (NPC) is the 4th most common malignancy in males in Hong Kong. The development of malignancy in this tumour involves a number of proteins and regulatory molecules in the cell cycle. Out past work has researched p21, p53 and p63 (Crook, et al. Oncogene 2000: 20:433-7) but in this proposal we will focus on the protein 14-3-3 s; the presence or absence of 14-3-3 s will be analysed by immunohistochemistry and hypermethylation status of the tumour DNA by methylation specific PCR(MSP).

Project Title:	Ultrastructural characterization of the SARS coronavirus (SARS-CoV)
Investigator(s):	Dr. Nicholls J.M., Dr. Chan K.H.
Department:	Pathology
Source(s) of Funding:	SARS Research Fund
Start Date:	07/2003

Abstract:

To determine the time course of SARS-CoV assembly in infected FRhK-4 and Vero cells; to analyze the formation of syncitia by SARS-CoV in cell culture; to detect changes in SARS-CoV formation under the effect of chemotherapeutic agents that block microtubule formation; to view the antigenic determinants of antibodies to the SARS-CoV.

List of Research Outputs

Chua D.T.T., Nicholls J.M., Sham J.S.T. and Au G.K.H., Prognostic Value of Epidermal Growth factor Receptor Expression in Patients with Advanced Stage Nasopharyngeal Carcinoma Treated with Induction Chemotherapy and Radiotherapy, International Journal of Radiation Oncology Biology Physcis. USA, Elsevier, 2004, 59: 11-20.

Nicholls J.M., Hahn P., Kremmer E., FrÖhlich T., Arnold G.J., Sham J.S.T., Kwong D.L.W. and Grässer F.A., Detection of wild type and deleted latent membrane protein 1 (LMP1) of Epstein-Barr virus in clinical biopsy material, Journal of Virological Methods. 2004, 116(1): 79-88.

Peiris J.S.M., Yu W.C., Leung C.W., Cheung C.Y., Ng W.F., Nicholls J.M., Ng T.K., Chan K.H., Lai S.T., Lim W.W.L., Yuen K.Y. and Guan Y., Re-emergence of fatal human influenza A subtype H5N1 disease, Lancet. 2004, 363(9409): 617-619.

Poon L.L.M., Chan K.H., Nicholls J.M., Zheng B., Yuen K.Y., Guan Y. and Peiris J.S.M., Characterization of a novel coronavirus responsible for severe acute respiratory syndrome, Options for the Control of Influenza V. 2004, International Congress Series 1263: 805-808.

Ren Y., Chan H.M., Li Z., Lin C.L., Nicholls J.M., Chan G.C.F., Lee P.Y., Lui V.C.H., Bacher M. and Tam P.K.H., Upregulation of macrophage migration inhibitory factor contributes to induced N-Myc expression by the activation of ERK signaling pathway and increased expression of interleukin-8 and VEGF in neuroblastoma, Oncogene. UK, Nature Publishing Group, 2004, 23: 4146-4154.

Researcher : Shek TWH

List of Research Outputs

Au W.Y., Ho K.S. and Shek T.W.H., Papillary renal cell carcinoma and gastrointestinal stromal tumor: a novel association, Annals of Oncology. 2004, 15: 843-844.

Chan C.P., Shek T.W.H. and Lee C.P., First-trimester diagnosis of hydrolethalus syndrome in a Chinese family, Prenatal Diagnosis. 2004, 24: 587-590.

Chan O.O., Ng I.O.L., Lam C.M., Shek T.W.H. and Lai C.L., Cholestatic jaundice caused by sequential carbimazole and propylthiouracil treatment for thyrotoxicosis, Hong Kong Medical Journal. 2003, 9(5): 377-380.

Chim J.C.S., Shek T.W.H., Chung L.P. and Liang R.H.S., Multiple lymphomatous polyposis in lymphoma of colon, Journal of Clinical Oncology. 2003, 953 – 955.

Hon C., Au W.Y., Shek T.W.H. and Shek T.W.H., Isolated sphenoid plasmacytoma presenting as painful ophthalmoplegia, Haematologica. 2003, 88:(08): EIM10.

Hon C., Kwok A.K., Shek T.W.H. and Au W.Y., Unusual locations of involvement by malignancies: Case 4. Bilateral hypopyon heralding CNS relapse of cutaneous natural killer cell lymphoma, Journal of Clinical Oncology. 2003, 21(17): 3373-4.

Lee C.K., Ma E.S.K., Shek T.W.H., Lam C.C.K., Au W.Y., Wan T.S.K. and Chan L.C., Plasmablastic transformation of multiple myeloma, Human Pathology. 2003, 34(7): 710-714.

Ng I.O.L., Chan K.L., Shek T.W.H., Lee M.F., Fong D.Y.T., Lo C.M. and Fan S.T., High frequency of chimerism in transplanted livers (Abstract), The 5th Annual Scientific Meeting, Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 3 January 2004.

Ng I.O.L., Chan K.L., Shek T.W.H., Lee J.M., Fong D.Y.T., Lo C.M. and Fan S.T., High frequency of chimerism in transplanted livers, Hepatology. 2003, 38(4): 989-998.

Ng K.C., Wong Y., Chan Y.K., Wong L.L.S., Tso W.K., Cheung K.M.C., Luk K.D.K., Shek T.W.H. and Chan F.L., Fluoroscopic guided vertebral bone biopsy: correlation with histopathological and microbiological results, Hong Kong Orthopaedic Association, Hong Kong, November 8-9, 2003.

Ng K.K.C., Lam C.M., Poon R.T.P., Shek T.W.H. and Fan S.T., Comparison of systemic responses of radiofrequency ablation, cryotherapy and surgical resection in a porcine liver model (Abstract), Annals of the College of Surgeons of Hong Kong. 2004, 8(Suppl.): A7.

Ng K.K.C., Lam C.M., Poon R.T.P., Shek T.W.H. and Fan S.T., Comparison of systemic responses of radiofrequency ablation, cryotherapy and surgical resection in a porcine liver model (Oral Presentation), The 11th Annual Scientific Meeting of the College of Surgeons of Hong Kong, Hong Kong, 5-6 June 2004.

Ng K.K.C., Lam C.M., Poon R.T.P., Shek T.W.H., Fan S.T. and Wong J., Delayed portal vein thrombosis after experimental radiofrequency ablation near the main portal vein, British Journal of Surgery. 2004, 91(5): 632-639.

Ng K.K.C., Lam C.M., Poon R.T.P., Shek T.W.H. and Fan S.T., Maximal host tolerance to hepatic radiofrequency ablation in a rat model (Abstract), The 5th Annual Scientific Meeting Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 3 January 2004.

Ng K.K.C., Lam C.M., Poon R.T.P., Shek T.W.H. and Fan S.T., Maximal host tolerance to large-volume hepatic radiofrequency ablation in a rat model (Abstract), Annals of the College of Surgeons of Hong Kong. 2004, 8(Suppl.): A7.

Ng K.K.C., Lam C.M., Poon R.T.P., Shek T.W.H. and Fan S.T., Maximal host tolerance to large-volume hepatic radiofrequency ablation in a rat model (Oral Presentation), The 11th Annual Scientific Meeting of the College of Surgeons of Hong Kong, Hong Kong, 5-6 June 2004.

Researcher : Shen L

List of Research Outputs

Shen L., Liang A.C.T., Lu L., Au W.Y., Wong K.Y., Tin P.C., Chan K.W., Ko K.H., Chen W.Y.W., Beh S.L., Shimizu N., Tsuchiyama T., Tang J.C.O., Kwong Y.L., Liang R.H.S. and Srivastava G., Aberrant BCL10 nuclear expression in nasal NK/T-cell lymphoma, Blood. 2003, 102(4): 1553-1554.

Shen L., Liang A.C.T., Au W.Y., Lu L., Chen W.Y.W., Wong K.Y., Tang J.C.O., Chan K.W., Beh S.L., Kwong Y.L., Liang R.H.S. and Srivastava G., BCL10 mutations are irrelevant to its aberrant nuclear localization in nasal NK/T-cell lymphoma, Leukemia. 2003, 17(11): 2240-2242.

Shen L., Immune escape mechanisms in EBV-associated nasal NK/T-cell lymphoma. 2004.

Researcher : Srivastava G

Project Title:	To investigate the role of immunoglobulin heavy and light chain k and l gene translocations in the pathogenesis of gastric B-cell non-Hodgkin's lymphoma
Investigator(s):	Prof. Srivastava G.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2002

Abstract:

To identify the novel translocation partner genes involving the IGH, IGk and IGl gene loci in GL by LDI-PCR method that allows the amplification of these translocations, and further analyze the regulation of the expression of these newly identified genes during B-cell differentiation.

Project Title:	Cloning of novel translocation partner genes involving immunoglobulin heavy chain gene locus in gastric lymphoma by long-distance inverse PCR
Investigator(s):	Prof. Srivastava G., Prof. Liang R.H.S., Dr. Lu L.W.
Department:	Pathology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	07/2003

Abstract:

To clone the translocation partner genes involving the IGH gene locus in GL by LDI-PCR methods and determine the frequency of these novel translocations in GL by direct PCR on chromosomal DNA; to study the expression of novel IGH translocation partner genes in GL by RT-PCR, ISH and immunostaining to determine whether the over expression of these genes is associated with the IGH translocation of these genes in GL; to determine the mRNA and protein expression of novel IGH fusion partner genes in human B-lineage cell lines that correspond to different stage of B-cell development and in primary B cells in reactive tonsils and lymph node. Loss of the normal control mechanisms regulating expression of these novel IGH partner genes may contribute to malignant transformation in lymphomas; to compare the levels of promoter activities by luciferase assays in COS-7 cells between IGH and the promoter of novel translocation partner genes and investigate by transfection experiments whether these IGH translocations do indeed result in the over expression of the translocation partner genes identified in this study by promoter substitution, due, in part, to the presence of potent B cell-specific transcriptional enhancers wihtin the IGH loci; to dertermine the transforming and tumorigenic potential of each of the overexpressed novel IGH translocation partner genes by in vitro growth properties and in vivo tumorigenicity assays; to analyze the mutations of incoming novel IGH translocation partner genes in GL since in B cells where the somatic hypermutation mechanism is active, mutations of the incoming gene may be observed and may contribute to the neoplastic phenotype; to investigate whether other heterologous promoters may also deregulate the involved genes of IGH translocation by fusion in GL by employing 5' RACE (rapid amplification of cDNA ends) strategy; to correlate the detection of novel IGH translocations with clinco-pathological features.

Project Title:	Study of epigenetic inactivation of ATM gene in mantle cell lymphoma
Investigator(s):	Prof. Srivastava G.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2003

Abstract:

To study the importance of methylation of the ATM gene in MCL lymphomagenesis; to study the prognostic impact of ATM methylation in MCL.

List of Research Outputs

Chen Y.W., Liang A.C.T., Au W.Y., Chu K.M., Wong K.Y., Hu X., Lu L., Tang J.C.O., Chan K.W., Beh S.L., Kwong Y.L., Liang R.H.S. and Srivastava G., Multiple BCL6 translocation partners in individual cases of gastric lymphoma, Blood. 2003, 102: 1931-1932.

Chim J.C.S., Wong K.Y., Loong F. and Srivastava G., SOCS1 and SHP1 hypermethylation in mantle cell lymphoma and follicular lymphoma: implications for epigenetic activation of the Jak/STAT pathway, Leukemia. 2004, 18(2): 356-372.

Kwong F.M., Tang J.C.O., Srivastava G. and Lung M.L., Inactivation mechanisms and growth suppressive effects of p16INK4a in Asian esophageal squamous carcinoma cell lines, Cancer Letters. 2004, 208(2): 207-213.

Law B.F.F., Srivastava G., Wong K.Y., Chen W.Y.W., Liang A.C.T., Lam K.Y., Law S.Y.K., Wong J. and Tang J.C.O., A novel tumor transforming gene at 7q22, GAESCC1, is frequently amplified and overexpressed in primary esophageal squamous cell carcinomas, Abstract of the Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Murray P.G., Qiu G., Fu L., Waites E.R., Srivastava G., Heys D., Agathanggelou A., Latif F., Grundy R.G., Mann J.R., Starczynski J., Crocker J., Parkes S.E., Ambinder R.F., Young L.S. and Tao Q., Frequent epigenetic inactivation of the RASSF1A tumor suppressor gene in Hodgkin's lymphoma, Oncogene. 2004, 23(6): 1326-1331.

Qiu G.H., Tan L.K., Loh K.S., Lim C.Y., Srivastava G., Tsai S.T., Tsao G.S.W. and Tao Q., The candidate tumor suppressor gene BLU, located at the commonly deleted region 3p21.3, is an E2F-regulated, stress-responsive gene and inactivated by both epigenetic and genetic mechanisms in nasopharyngeal carcinoma, Oncogene. 2004, 1-14.

Shen L., Liang A.C.T., Lu L., Au W.Y., Wong K.Y., Tin P.C., Chan K.W., Ko K.H., Chen W.Y.W., Beh S.L., Shimizu N., Tsuchiyama T., Tang J.C.O., Kwong Y.L., Liang R.H.S. and Srivastava G., Aberrant BCL10 nuclear expression in nasal NK/T-cell lymphoma, Blood. 2003, 102(4): 1553-1554.

Shen L., Liang A.C.T., Au W.Y., Lu L., Chen W.Y.W., Wong K.Y., Tang J.C.O., Chan K.W., Beh S.L., Kwong Y.L., Liang R.H.S. and Srivastava G., BCL10 mutations are irrelevant to its aberrant nuclear localization in nasal NK/T-cell lymphoma, Leukemia. 2003, 17(11): 2240-2242.

Srivastava G., Immune escape mechanisms in nasal NK/T-cell lymphoma- mutations in pro-apoptotic genes, Abstract of the 6th International Conference of the Asian Clinical Oncology Society (ACOS), Seoul, South Korea, 16-19 November 2003.

Yang L.C., Tang J.C.O., Srivastava G., Stanbridge E.J. and Lung M.L., Functional investigation of tumor suppressive role of chromosome 9 in esophageal squamous cell carcinoma, Abstract of the Proceedings of 40th Annual Meeting of the American Society of Clinical Oncology (ASCO), New Orleans, USA, 5-8 June 2004.

Ying J., Srivastava G., Gao Z., Zhang X., Murray P., Ambinder R. and Tao Q., Promoter hypermethylation of the cyclin-dependent kinase inhibitor (CDKI) gene p21^{WAF1/CIP1/SDI1} is rare in various lymphomas and carcinomas, Blood. 2004, 103(2): 743-766.

Zhang M., Srivastava G. and Lu L., The pre-B cell receptor and its function during B cell development, Cellular and Molecular Immunology. 2004, 1(2): 89-94.

Zhang X.H., Tao Q., Srivastava G. and Gao Z., Hypermethylation of tumor suppressor genes BLU and ARF in nasal lymphoma, Chin J Intern Med. 2003, 42: 125-126.

Researcher : Sze MF

List of Research Outputs

Sze M.F., Ching Y.P., Jin D. and Ng I.O.L., Dysregulation of mitotic spindle checkpoint control in hepatocellular carcinomas, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Researcher : Szeto EF

List of Research Outputs

Cheung A.N.Y., Szeto E.F., Ng K.M., Fong K.W., Ang Chan E.Y., Tsun O.K.L., Khoo U.S., Chan Y.K. and Ng W.Y., Atypical squamous cells of undetermined significance on cervical smears: follow-up study of an Asian screening population, Cancer Cytopathology. 2004, 102(2): 74-80.

Cheung A.N.Y., Szeto E.F., Leung B.S.Y., Khoo U.S. and Ng W.Y., Liquid-based cytology and conventional cervical smears: a comparison study in an Asian screening population, Cancer. 2003, 99(6): 331-335.

Researcher : Tam SCF

List of Research Outputs

Cheung B.M.Y., Lam T.H., Lam K.S.L., Tam S.C.F., Wat N.M.S., Lo L.F., Chau F.Y., Law C.Y., Man Y.B., Cheng C.H., Kumana C.R. and Lau C.P., Follow up of the Hong Kong Cardiovascular risk factor prevalence survey cohort, Hong Kong Medical Journal. 2003, 9(1) Suppl: 27.

Cheung B.M.Y., Man Y.B., Lo L.F., Lo L.F., Chau F.Y., Law C.Y., Lam K.S.L., Lam T.H., Wat N.M.S., Tam S.C.F., Cheng C.H., Kumana C.R. and Lau C.P., High blood pressure is related to obesity in Hong Kong, Hong Kong Medical Journal. 2003, 9(1) Suppl: 35.

Cheung B.M.Y., Man Y.B., Lo L.F., Chau F.Y., Law C.Y., Lam K.S.L., Lam T.H., Wat N.M.S., Tam S.C.F., Cheng C.H., Kumana C.R. and Lau C.P., Lipid profile of the Hong Kong cardiovascular risk factor prevalence study cohort, Hong Kong Medical Journal. 2003, 9(1) Suppl: 22.

Cheung B.M.Y., Man Y.B., Lam K.S.L., Wat N.M.S., Lo L.F., Chau F.Y., Law C.Y., Lam T.H., Leung G.M., Tam S.C.F., Cheng C.H., Kumana C.R. and Lau C.P., Prevalence of diabetes mellitus (DM) in the Hong Kong Cardiovascular risk factor prevalence study cohort, Hong Kong Medical Journal. 2003, 9(1) Suppl: 19.

Cheung B.M.Y., Man Y.B., Lo L.F., Chau F.Y., Law C.Y., Lam K.S.L., Lam T.H., Wat N.M.S., Tam S.C.F., Cheng C.H., Kumana C.R. and Lau C.P., Prevalence of obesity in the Hong Kong Cardiovascular risk factor prevalence survey-2 (CRISPS2), Hong Kong Medical Journal. 2003, 9(1) Suppl: 23.

Jin O., Tan K.C., Yeung J.S.L., Tam S.C.F. and Lau W.C.S., Influence on hyperlipidaemia on macrophage phagocytosis of apoptotic cells, 7th International Congress on SLE & Related Conditions, New York. 2004.

Researcher : Tam YSI

List of Research Outputs

Choi K.C., Wong M.P., Tam Y.S.I., Lam C.L., Lam W.K., Wang E., Chau W.S., Chiu S.W. and Chung L.P., Angiogenic activity and angiogenesis-related factor expression in non-small cell lung cancers, Proceedings of the 10th Hong Kong International Cancer Congress, 19-21 November 2003.

Li T.C., Wong M.P., Tam Y.S.I., Lam C.L., Lam W.K., Wang E., Chau W.S., Chiu S.W. and Chung L.P., Promoter hypermethylation of MGMT, RARB, DAPK and CDH13 genes in non-small cell lung cancers (NSCLC) in Hong Kong, Proceedings of the 10th Hong Kong International Cancer Congress, 19-21 November 2003.

Researcher : Tang JCO

List of Research Outputs

Chen Y.W., Liang A.C.T., Au W.Y., Chu K.M., Wong K.Y., Hu X., Lu L., Tang J.C.O., Chan K.W., Beh S.L., Kwong Y.L., Liang R.H.S. and Srivastava G., Multiple BCL6 translocation partners in individual cases of gastric lymphoma, Blood. 2003, 102: 1931-1932.

Kwong F.M., Tang J.C.O., Srivastava G. and Lung M.L., Inactivation mechanisms and growth suppressive effects of p16INK4a in Asian esophageal squamous carcinoma cell lines, Cancer Letters. 2004, 208(2): 207-213.

Law B.F.F., Srivastava G., Wong K.Y., Chen W.Y.W., Liang A.C.T., Lam K.Y., Law S.Y.K., Wong J. and Tang J.C.O., A novel tumor transforming gene at 7q22, GAESCC1, is frequently amplified and overexpressed in primary esophageal squamous cell carcinomas, Abstract of the Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Shen L., Liang A.C.T., Lu L., Au W.Y., Wong K.Y., Tin P.C., Chan K.W., Ko K.H., Chen W.Y.W., Beh S.L., Shimizu N., Tsuchiyama T., Tang J.C.O., Kwong Y.L., Liang R.H.S. and Srivastava G., Aberrant BCL10 nuclear expression in nasal NK/T-cell lymphoma, Blood. 2003, 102(4): 1553-1554.

Shen L., Liang A.C.T., Au W.Y., Lu L., Chen W.Y.W., Wong K.Y., Tang J.C.O., Chan K.W., Beh S.L., Kwong Y.L., Liang R.H.S. and Srivastava G., BCL10 mutations are irrelevant to its aberrant nuclear localization in nasal NK/T-cell lymphoma, Leukemia. 2003, 17(11): 2240-2242.

Yang L.C., Tang J.C.O., Srivastava G., Stanbridge E.J. and Lung M.L., Functional investigation of tumor suppressive role of chromosome 9 in esophageal squamous cell carcinoma, Abstract of the Proceedings of 40th Annual Meeting of the American Society of Clinical Oncology (ASCO), New Orleans, USA, 5-8 June 2004.

Researcher : Tin PC

List of Research Outputs

Shen L., Liang A.C.T., Lu L., Au W.Y., Wong K.Y., Tin P.C., Chan K.W., Ko K.H., Chen W.Y.W., Beh S.L., Shimizu N., Tsuchiyama T., Tang J.C.O., Kwong Y.L., Liang R.H.S. and Srivastava G., Aberrant BCL10 nuclear expression in nasal NK/T-cell lymphoma, Blood. 2003, 102(4): 1553-1554.

Researcher : To KW

List of Research Outputs

Huang F., Ma L., Wu A.Y.Y., Tian L., Chan K.W., Chan A.K., Lam A.C., Lo C.K., Ko K.H., To K.W., Chik S., Kam S.K., Fong I., Li X.S., Ng M.H. and MacPherson G.G., Systemic autoimmune disease induced by dendritic cells that have captured dying cells, Proceedings of the Frontiers in Biomedical Research, Hong Kong. 12 December 2003.

Ma L., Chan A.K., Lam A.C., Chan K.W., Lo C.K., Ko K.H., To K.W., Wu A.Y.Y., Tian L., Ng M.H. and Huang F., The role of dendritic cells in the induction of systemic autoimmune disease, Proceedings of the 8th Research Postgraduate Symposium, Hong Kong, 13 December 2003.

Researcher : Tsui WY

List of Research Outputs

Chan A.S.W., Tsui W.Y., Chen X., Chu K.M., Chan T.L., Chan A.S.Y., Li R., So S., Yuen S.T. and Leung S.Y., Down-regulation of ID4 by promoter hypermethylation in gastric adenocarcinoma, Proceedings of the International Conference on Applied Genomics, 9th European Society for Analytical Cellular Pathology meeting and 16th International Society for Diagnostic Quantitative Pathology meeting, Netherlands, 2003.

Chan A.S.W., Tsui W.Y., Chen X., Chu K.M., Chan T.L., Chan A.S.Y., Li R., So S., Yuen S.T. and Leung S.Y., Downregulation of ID4 by promoter hypermethylation in gastric adenocarcinoma, Oncogene. 2003, 22(44): 6946-6953.

Researcher : Tsun OKL

List of Research Outputs

Cheung A.N.Y., Szeto E.F., Ng K.M., Fong K.W., Ang Chan E.Y., Tsun O.K.L., Khoo U.S., Chan Y.K. and Ng W.Y., Atypical squamous cells of undetermined significance on cervical smears: follow-up study of an Asian screening population, Cancer Cytopathology. 2004, 102(2): 74-80.

Researcher : Wan TSK

List of Research Outputs

Au W.Y., Ooi C.G.C., Yau W.F., Ma E.S.K., Wan T.S.K. and Kwong Y.L., Chronic myeloid leukemia in an adolescent with Ollier’s diease after intensive X ray exposure, Leukemia and Lymphoma. 2004, 45: 613-616.

Au W.Y., Ma E.S.K., Wan T.S.K., Man C.W.Y. and Kwong Y.L., Pentasomy 8q after cyclophosphamide therapy for fibrosing alveolitis: case report and review, Cancer Genetics and Cytogenetics. 2003, 141: 79-82.

Jin Y., Zhang H., Tsao G.S.W., Jin C., Lv M., Strombeck B., Wiegant J., Wan T.S.K., Yuen P.W. and Kwong Y.L., Cytogenetic and molecular genetic characterization of immortalized human ovarian surface epithelial cell lines: consistent loss of chromosome 13 and amplification of chromosome 20, Gynecologic Oncology. 2004, 92: 183-191.

Lee C.K., Ma E.S.K., Shek T.W.H., Lam C.C.K., Au W.Y., Wan T.S.K. and Chan L.C., Plasmablastic transformation of multiple myeloma, Human Pathology. 2003, 34(7): 710-714.

Wan T.S.K., Ma E.S.K., Li C.K. and Chan L.C., Atypical fluorescence in situ hybridisation pattern in chronic myeloid leukaemia due to cryptic insertion of BCR at 9q34, Leukemia. 2004, 18(1): 161-162.

Wan T.S.K., Ma E.S.K., Lam C.C.K., Chan L.C., Lee K.K. and Au W.Y., Deletion 9q as the sole karyotypic abnormality in myelocytic disorders: a new case of myelodysplastic syndrome and its prognostic implications in acute myelocytic leukemia, Cancer Genetics and Cytogenetics. 2003, 145(2): 184-186.

Researcher : Wong CM

List of Research Outputs

Chan C.F., Yau T.O., Jin D., Wong C.M., Fan S.T. and Ng I.O.L., Evaluation of nuclear factor-kB, urokinase-type plasminogen activator, and HBx and their clinicopathological significance in hepatocellular carcinoma, Clinical Cancer Research. 2004, 10(12 pt. 1): 4140-4149.

Leung H.Y., Ching Y.P., Wong C.M., Ng D.C.H., Jin D. and Ng I.O.L., Characterization of novel putative tumor suppressor gene, DLC2 (deleted in liver cancer 2), in hepatocellular carcinoma, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Pang R.W.C., Yuen J.C.H., Yuen M.F., Lai C.L., Lee K.W., Man K., Poon R.T.P., Fan S.T., Wong C.M., Ng I.O.L., Kwong Y.L. and Tse E.W.C., PIN1 over-expression and β-catenin gene mutations are distinct oncogenic events in human hepatocellular carcinoma, Oncogene. 2004, 23(23): 4182-6.

Wong C.M., Ching Y.P., Lee M.F., Jin D. and Ng I.O.L., Genetic and epigenetic alterations of DLC-1 gene in hepatocellular carcinoma, Cancer Research. 2003, 63: 7646-7651.

Wong C.M., Ph.D. Thesis, Novel candidate tumour suppressor genes, DLC-1 and DLC-2, in hepatocellular carcinoma. 2003.

Researcher : Wong CM

List of Research Outputs

Wong C.M., Ching Y.P., Lee M.F., Jin D. and Ng I.O.L., Genetic and epigenetic alterations of DLC-1 gene in hepatocellular carcinoma, Cancer Research. 2003, 63: 7646-7651.

Wong C.M., Ph.D. Thesis, Novel candidate tumour suppressor genes, DLC-1 and DLC-2, in hepatocellular carcinoma. 2003.

Researcher : Wong CW

List of Research Outputs

Chan T.L., Wong C.W., Chan A.S.W., Guo D., Ho J.W.C., Yuen S.T. and Leung S.Y., BRAF and KRAS mutations in mismatch repair deficient colorectal cancer , Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Researcher : Wong KY

List of Research Outputs

Chen Y.W., Liang A.C.T., Au W.Y., Chu K.M., Wong K.Y., Hu X., Lu L., Tang J.C.O., Chan K.W., Beh S.L., Kwong Y.L., Liang R.H.S. and Srivastava G., Multiple BCL6 translocation partners in individual cases of gastric lymphoma, Blood. 2003, 102: 1931-1932.

Chim J.C.S., Wong K.Y., Loong F. and Srivastava G., SOCS1 and SHP1 hypermethylation in mantle cell lymphoma and follicular lymphoma: implications for epigenetic activation of the Jak/STAT pathway, Leukemia. 2004, 18(2): 356-372.

Law B.F.F., Srivastava G., Wong K.Y., Chen W.Y.W., Liang A.C.T., Lam K.Y., Law S.Y.K., Wong J. and Tang J.C.O., A novel tumor transforming gene at 7q22, GAESCC1, is frequently amplified and overexpressed in primary esophageal squamous cell carcinomas, Abstract of the Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Shen L., Liang A.C.T., Lu L., Au W.Y., Wong K.Y., Tin P.C., Chan K.W., Ko K.H., Chen W.Y.W., Beh S.L., Shimizu N., Tsuchiyama T., Tang J.C.O., Kwong Y.L., Liang R.H.S. and Srivastava G., Aberrant BCL10 nuclear expression in nasal NK/T-cell lymphoma, Blood. 2003, 102(4): 1553-1554.

Shen L., Liang A.C.T., Au W.Y., Lu L., Chen W.Y.W., Wong K.Y., Tang J.C.O., Chan K.W., Beh S.L., Kwong Y.L., Liang R.H.S. and Srivastava G., BCL10 mutations are irrelevant to its aberrant nuclear localization in nasal NK/T-cell lymphoma, Leukemia. 2003, 17(11): 2240-2242.

Researcher : Wong MP

Project Title:	Genetic and epigenetic changes of RASSF1 in lymphoepithelioma-like carcinoma of lung
Investigator(s):	Dr. Wong M.P., Dr. Chung L.P.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2001
Completion Date:	10/2003

Abstract:

To study the genetic and epigenetic changes of the tumour suppressor gene, RASSF1, in primary lymphoepithelioma-like carcinomas (LELC) of the lung.

Project Title:	Analysis of genetic changes in non-small cell lung cancers from non-smokers in Hong Kong
Investigator(s):	Dr. Wong M.P., Dr. Chiu S.W., Dr. Chung L.P., Prof. Lam W.K.
Department:	Pathology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	01/2002

Abstract:

To identify loci of significant DNA gain, implicating the presence of potential oncogences, or loss, implicating the presence of potential tumour suppressor genes (TSG) in NSCLC from non-smokers; to identify genes or DNA sequences that show up -or down- regulated expression levels in NSCLC of non-smokers compared to normal lung; to identify and analyse candidate oncogenes or TSG for activating mutations or inactivating genetic or epigenetic alterations.

Project Title:	Lymphocytic BPDE-DNA adduct level and its relation with genomic aberrations in primary non-small cell lung carcinomas from smokers and non-smokers
Investigator(s):	Dr. Wong M.P., Dr. Chung L.P., Dr. Lu L.W.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2002

Abstract:

To study the peripheral blood lymphocytic BPDE-DNA adduct level and its relation with genomic aberrations in primary non-small cell lung carcinomas of Hong Kong.

Project Title:	Analysis of the pathology and pathogenesis in the lungs of patients suffering from the Severe Acute Respiratory Syndrome (SARS)
Investigator(s):	Dr. Wong M.P., Dr. Beh P.S.L., Dr. Chung L.P., Dr. Lee K.C., Dr. Leung C.Y., Dr. Nicholls J.M., Dr. Pang S.W.
Department:	Pathology
Source(s) of Funding:	SARS Research Fund
Start Date:	07/2003

Abstract:

To evaluate the pathological changes in the lung in patients suffering from SARS.

Project Title:	Identification of candidate cancer genes in regions of frequent chromosomal aberration in non-small cell lung cancers
Investigator(s):	Dr. Wong M.P., Dr. Chiu S.W., Dr. Chung L.P., Prof. Lam W.K.
Department:	Pathology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	09/2003

Abstract:

To define critical regions of imbalance in frequently altered chromosomal loci identified in local samples of non-small cell lung cancers (NSCLC) by microstatellite analysis; to identify target genes in the analyzed regions by comparison with genes listed in databases of the Human Genome Resources(NCBI); to study the structure and expression of the targeted genes in NSCLC of smokers and non-smokers, so as to verify their involvement and to look for genetic or epigenetic alterations.

Project Title:	Identification of candidate cancer genes in regions of frequent chromosomal aberration in non-small cell lung cancers
Investigator(s):	Dr. Wong M.P., Dr. Chiu S.W., Dr. Chung L.P., Prof. Lam W.K.
Department:	Pathology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	09/2003

Abstract:

Project Title:	Analysis of allelic imbalance at 16q24 and WW domain-containing oxidoreductase (WWOX) alterations in primary non-small cell lung cancer in smokers and non-smokers
Investigator(s):	Dr. Wong M.P., Dr. Chung L.P.
Department:	Pathology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2003

Abstract:

To analysis allelic imbalance at 16q24; to analysis WWOX genetic and epigenetic alterations in lung cancers.

List of Research Outputs

Chan M.M.W., Lam W.K., Mak J.C.W., Ho S.P., Chan R.H.W., Tsang K.W.T., Ip M.S.M., Ho J.C.M., Wong M.P. and Tan-Un K.C., Polymorphisms of CYP and GST genes, passive smoke and lung cancer risk in Hong Kong Chinese, 9th Medical Research Conference, Medical Science Group, The University of Hong Kong. 2004, Abstract Book: RC-11.

Choi K.C., Wong M.P., Tam Y.S.I., Lam C.L., Lam W.K., Wang E., Chau W.S., Chiu S.W. and Chung L.P., Angiogenic activity and angiogenesis-related factor expression in non-small cell lung cancers, Proceedings of the 10th Hong Kong International Cancer Congress, 19-21 November 2003.

Ho J.C.M., Lam W.K., Lam B., Wong M.P. and Tsang K.W.T., Clinical outcome for lymphoepithelioma-like carcinoma of the lung cancer after chemoradiotherapy, Lung Cancer. 2003, 41 (suppl 2): S146.

Ho J.C.M., Lam W.K., Ooi C.G.C., Wong M.P., Lam J.C., Ip M.S.M. and Tsang K.W.T., Lymphoepithelioma-like carcinoma of the lung in a patient with silicosis, European Respiratory Journal . 2003, 22(2): 383-386.

Ho J.C.M., Ho S.P., Chan M.M.W., Mak J.C.W., Ip M.S.M., Wong M.P., Tsang K.W.T. and Lam W.K., Non-small cell lung cancer in Chinese is associated with disturbance in systemic antioxidants profiles , 9th Medical Research Conference, Medical Science Group, The University of Hong Kong. 2004, Abstract Book: RC-03.

Ho J.C.M., Ho S.P., Chan M.M.W., Ip M.S.M., Wong M.P., Tsang K.W.T. and Lam W.K., Systemic antioxidant profiles in non-small cell lung cancer in Chinese, The Journal of the Japanese Respiratory Society. 2004, 42 (suppl): 69.

Lam B., Ooi C.G.C., Wong M.P., Lee R., Ip M.S.M., Chan M.M.W., Lam W.K. and Tsang K.W.T., Extrapulmonary presentation of asymptomatic pulmonary lymphangioleiomyomatosis, Respirology. 2003, 8(4): 544-547.

Lam C.L., Wong M.P., Lam W.K., Chung L.P., Chiu S.W., Chau W.S., Ho K.K. and Minna J.D., Quantitative comparison of expression of nicotinic acetylcholine receptor (nAChR)subunits between non-small cell lung cancers (NSCLCs) arising in life-time never-smokers and smokers in Chinese patients , Research Postgraduate Symposium Presentation, The University of Hong Kong. 2003, Abstract Book.

Lam D.C.L., Wong M.P., Chung L.P., Girard L., Lam W.K. and Minna J.D., Expression profiling with microarray in studying differential gene expression in different primary lung cancer cell lines newly established from local Chinese patients, 9th Medical Research Conference, Medical Science Group, The University of Hong Kong. 2004, Abstract Book: RC-15.

Lam D.C.L., Wong M.P., Lam W.K., Chung L.P., Chiu S.W., Chau W.S., Ho K.K. and Minna J.D., Quantitative comparison of expression of nicotinic acetylcholine receptor (nAChR)subunits between non-small cell lung cancer (NSCLC) arising in life-time never-smokers in Chinese patients , Lung Cancer. 2003, 41 (suppl 2): S89.

Lam D.C.L., Wong M.P., Lam W.K., Chung L.P., Chiu S.W., Chau W.S., Ho K.K. and Minna J.D., Quantitative comparison of nicotinic acetylcholine receptor subunit expression level reveals differences in non-small cell lung cancer arising fron life-time never-smokers and smokers, 9th Medical Research Conference, Medical Science Group, The University of Hong Kong. 2004, Abstract Book: RC-14.

Li T.C., Wong M.P., Tam Y.S.I., Lam C.L., Lam W.K., Wang E., Chau W.S., Chiu S.W. and Chung L.P., Promoter hypermethylation of MGMT, RARB, DAPK and CDH13 genes in non-small cell lung cancers (NSCLC) in Hong Kong, Proceedings of the 10th Hong Kong International Cancer Congress, 19-21 November 2003.

Wong M.P., Tipoe G.L., Sun J.Z., Mak J.C.W., Ooi C.G.C., Ip M.S.M., Chan M.M.W., Lam W.K. and Tsang K.W.T., Clinical significance of ciliary abnormalities in bronchiectasis, Annual Scientific Meeting, Hong Kong Thoracic Society & American College of Chest Physicians, 2003. Abstract pp.30.

Wong M.P., Expression profiling with microarray in studying differential gene expression in different primary lung cancer cell lines newly established from local Chinese patients, Proceedings of the 9th Medical Research Conference, Medical Science Group, The University of Hong Kong, February 2004.

Wong M.P., Genome-wide screening of chromosomal imbalance in non-small cell lung cancer by array CGH, Proceedings of the 8th Research Postgraduate Symposium, Hong Kong, 13 December 2003.

Wong M.P., Molecular Oncology ABC – Lung Cancer, Proceedings of the 10th Hong Kong International Cancer Congress, 19-21 November 2003.

Zhu H., Wong M.P., Lam C.L., Cai W.W., Chau W.S., Wang E., Lam W.K., Chiu S.W. and Chung L.P., Genome-wide screening of chromosomal imbalance in NSCLC by array-based comparative genomic hybridization (array-CGH), Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Researcher : Xue W

List of Research Outputs

Chan Y.K., Shen D.H., Li S.S., Khoo U.S., Ngan H.Y.S., Xue W. and Cheung A.N.Y., Differential Expression and Methylation of p33/ING1b in Ovarian Tumors, Proceedings of the 94th Annual Meeting of the American Association for Cancer Research, Washington, USA, 11-14 July 2003.

Chan Y.K., Song Y.Q., Mak W., Cheung A.N.Y., Peiris J.S.M., Yip S.P., Tin V., Liu W., Xue W., Chan L.C., Tam P., Ngan H.Y.S. and Khoo U.S., Polymorphisms of the ACE2 and CD13 genes, and susceptibility to SARS coronavirus infection in Chinese from Hong Kong, Abstract for Human Genome Organization (HUGO) Meeting, April 4-7, 2004, Berlin, Germany. 2004.

Chan Y.K., Khoo U.S., Chan K.H.K., Ngan H.Y.S., Li S.S., Xue W., Chiu P.M. and Cheung A.N.Y., Reduced GSTP1 immunoreactivity in endometrial carcinomas in association with methylation status and genetic profiles, Proceedings of the 94th Annual Meeting of the American Association for Cancer Research, Washington, USA, 11-14 July 2003.

Cheung A.N.Y., Khoo U.S., Lai C.Y.L., Chan Y.K., Xue W., Cheng D.K.L., Chiu P.M., Tsao G.S.W. and Ngan H.Y.S., Metastatic trophoblastic disease after an initial diagnosis of partial hydatidiform mole: genotyping and chromosome in situ hybridization analysis, Cancer. 2004, 100(7): 1411-1417.

Cheung A.N.Y., Xue W., Chan D.Y., Feng H., Chiu P.M., Tsao G.S.W. and Ngan H.Y.S., Promoter hypermethylation of multiple genes in hydatiform mole choriocarcinoma, Proceedings of the American Association for Cancer Research. 2004, 45: 1149 Abstract No. 4974.

Cheung A.N.Y., Khoo U.S., Xue W. and Shen D.H., Recent advances in the research of gestational trophoblastic diseases, 妊娠滋??胞病?的研究?展, Chinese Journal of Pathology. 中?病理??志 , 2003, 32(4): 373-375.

Feng H., Xue W., Cheung A.N.Y., Wang Y.L. and Tsao G.S.W., Differentially expressed genes between ormal placenta and choriocarcinoma, Proceedings of the American Society for Cell Biology, 43rd Annual Meeting. 2003, Abstract No. 2774.

Feng H., Cheung A.N.Y., Xue W., Wang Y., Wang X., Fu S., Wang Q., Ngan H.Y.S. and Tsao G.S.W., Down-regulation and promoter methylation of tissue inhibitor of metalloproteinase 3 in choriocarcinoma, Gynecologic Oncology. 2004, 94: 375-382.

Khoo U.S., Chan Y.K., Yip S.P., Peiris M., Tin V., Liu W., Xue W., Ngan H.Y.S., Chan L.C. and Cheung A.N.Y., The tandem repeat polymorphism of the DC-SIGNR gene is associated with susceptibility to SARS coronavirus infection in Chinese from Hong Kong, Proceedings of the Human Genome Organization (HUGO) Meeting, April 4-7, 2004, Berlin, Germany. 2004.

Lai C.Y.L., Chan Y.K., Khoo U.S., Ngan H.Y.S., Xue W., Chiu P.M., Tsao G.S.W. and Cheung A.N.Y., Analysis of gestational trophoblastic disease by genotyping and chromosome in situ hybridization, Modern Pathology. 2004, 17: 40-48.

Leung T.W., Xue W., Cheung A.N.Y., Khoo U.S. and Ngan H.Y.S., Proliferation to apoptosis ratio as a prognostic marker in adenocarcinoma of uterine cervix, Gynecologic Oncology. 2004, 92(3): 866-872.

Shen D., Khoo U.S., Ngan H.Y.S., Ng T.Y., Chau M.T., Xue W. and Cheung A.N.Y., Coexisting epithelioid trophoblastic tumor and choriocarcinoma of the uterus following a chemoresistant hydatidiform mole, Archives of Pathology and Laboratory Medicine. 2003, 127(7): e291–e293.

Xue W., Feng H., Tsao G.S.W., Chan Y.K., Ngan H.Y.S., Chiu P.M., Maccalman C.D. and Cheung A.N.Y., Methylation status and expression of E-cadherin and cadherin-11 in gestational trophoblastic diseases, International Journal of Gynecological Cancer. 2003, 13(6): 879-888.

Xue W., Khoo U.S., Ngan H.Y.S., Chan Y.K., Chiu P.M., Tsao G.S.W. and Cheung A.N.Y., Minichromosome maintenance protein 7 expression in gestational trophoblastic disease: correlation with Ki67, PCNA and clinicopathological parameters, Histopathology. 2003, 43: 485-490.

Xue W., Khoo U.S., Ngan H.Y.S., Chan Y.K., Ip P.P.C., Tsao G.S.W. and Cheung A.N.Y., c-mos immunoreactivity aids in the diagnosis of gestational trophoblastic lesions, International Journal of Gynecological Pathology. 2004, 23(2): 145-150.

Yang C., Ngan H.Y.S., Khoo U.S., Chan Y.K., Chiu P.M., Chan Q.K.Y., Xue W. and Cheung A.N.Y., Single nucleotide polymorphisms (SNPS) in the coding sequence of follicle stimulating hormone receptor (FSHR) is associated with ovarian cancer susceptibility, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Researcher : Xue W

List of Research Outputs

Researcher : Yam JWP

List of Research Outputs

Wong V.K.W., Yam J.W.P. and Hsiao W.L.W., Cloning and characterization of the promoter region of the mouse frizzled-related protein 4 gene, Biological Chemistry. 2003, 384(8): 1147-1154.

Yam J.W.P., Jin D., So C.W. and Chan L.C., Identification and characterization of EBP, a novel EEN binding protein that inhibits Ras signaling and is recruited into the nucleus by the MLL-EEN fusion protein, Blood. 2004, 103(4): 1445-1453.

Yam J.W.P. and Chan L.C., MLL-EEN fusion protein recruits the cytoplasmic EEN binding protein, EBP into the nucleus, Proceedings of the FASEB Summer Research Conferences – Hematological Malignancies, Vermont, USA, 26-31 July 2003.

Researcher : Yang C

List of Research Outputs

Yang C., Ngan H.Y.S., Khoo U.S., Chan Y.K., Chiu P.M., Chan Q.K.Y., Xue W. and Cheung A.N.Y., Single nucleotide polymorphisms (SNPS) in the coding sequence of follicle stimulating hormone receptor (FSHR) is associated with ovarian cancer susceptibility, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Researcher : Yuen ST

List of Research Outputs

Chan A.S.W., Tsui W.Y., Chen X., Chu K.M., Chan T.L., Chan A.S.Y., Li R., So S., Yuen S.T. and Leung S.Y., Down-regulation of ID4 by promoter hypermethylation in gastric adenocarcinoma, Proceedings of the International Conference on Applied Genomics, 9th European Society for Analytical Cellular Pathology meeting and 16th International Society for Diagnostic Quantitative Pathology meeting, Netherlands, 2003.

Chan A.S.W., Tsui W.Y., Chen X., Chu K.M., Chan T.L., Chan A.S.Y., Li R., So S., Yuen S.T. and Leung S.Y., Downregulation of ID4 by promoter hypermethylation in gastric adenocarcinoma, Oncogene. 2003, 22(44): 6946-6953.

Chan A.S.W., Chu K.M., Yuen S.T. and Leung S.Y., High-throughput quantification of hMLH1 promoter methylation using PyrosequencingTM technology , Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Chan G.S.W., Yuen S.T. and Chan K.W., Synovial sarcoma presenting as a polypoid jejunal mass, Histopathology. 2004, 44(2): 191-193.

Chan T.L., Zhao W., Cancer Genome Project .., Leung S.Y. and Yuen S.T., BRAF and KRAS mutations in colorectal hyperplastic polyps and serrated adenomas, Cancer Research. 2003, 63: 4878-4881.

Chan T.L., Wong C.W., Chan A.S.W., Guo D., Ho J.W.C., Yuen S.T. and Leung S.Y., BRAF and KRAS mutations in mismatch repair deficient colorectal cancer , Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Chan T.L., Chan Y.W., Ho J.W.C., Chan C., Chan A.S.Y., Chan E., Lam P.W.Y., Tse C.W., Lee K.C., Lau C.W., Gwi E., Leung S.Y. and Yuen S.T., MSH2 c.1452-1455delAATG is a founder mutation and an important cause of hereditary nonpolyposis colorectal cancer in the southern Chinese population, The American Journal of Human Genetics. 2004, 74(5): 1035-1042.

Chen X., Leung S.Y., Yuen S.T., Chu K.M., Ji J., Li R., Chan A.S.Y., Law S.Y.K., Troyanskaya O.G., Wong J., So S., Botstein D. and Brown P.O., Variation in gene expression patterns in human gastric cancers, Molecular Biology of the Cell. 2003, 14(8): 3208-3215.

Guo D., Yuen S.T., Ho J.W.C., Chan A.S.Y., Chen X. and Leung S.Y., PLA2G2A expression and its clinical significance in colorectal tumors, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

Ho J.W.C., Ho M.Y., Chan C.L.W., Yuen S.T., Chan M.S. and Siu H.Y.V., Psychological profile of hereditary colorectal cancer genetic screening test recipients in Hong Kong: results of the first 88 participants (Abstracts & Proceedings), Familial Cancer 2003: Research and Practice, Couran Cove, Australia, 3-6 September 2003.

Ko S., Chu K.M., Luk J.M.C., Wong W.Y., Leung S.Y., Yuen S.T. and Wong J., Expression of CDX2 and LI-cadherin in intestinal metaplasia and adenocarcinoma of the stomach, 95th Annual Meeting of the American Association for Cancer Research, Orlando, U.S.A., 27-31 March 2004.

Wong B.C.Y., Lam S.K., Wong R.W.M., Chen J.S., Zheng T., Feng R., Lai K.C., Hu H.C., Yuen S.T., Leung S.Y., Fong D.Y.T., Ho J.C.Y., Ching C.K. and Chen J.S., Helicobacter pylori Eradication to Prevent Gastric Cancer in a High-Risk Region of China: a Randomized Controlled Trial, The Journal of American Medical Association. 2004, 291(2): 187-194.

Wong B.C.Y., Lam S.K., Wong R.W.M., Chen J.S., Zheng T., Feng R., Lai K.C., Hu H.C., Yuen S.T., Leung S.Y., Fong D.Y.T., Ho J.W.C. and Ching C.K., Helicobacter pylori eradication to prevent gastric cancer in a high-risk region in China: A randomized controlled trial, JAMA. 2004, 291(2): 187-194.

Zhao W., Chan T.L., Chu K.M., Chan A.S.Y., Stratton M.R., Yuen S.T. and Leung S.Y., Mutations of BRAF and KRAS in gastric cancer and their association with microsatellite instability, International Journal of Cancer. 2004, 108(1): 167-169.

Researcher : Zhang M

List of Research Outputs

Zhang M., Srivastava G. and Lu L., The pre-B cell receptor and its function during B cell development, Cellular and Molecular Immunology. 2004, 1(2): 89-94.

Researcher : Zhao W

List of Research Outputs

Chan T.L., Zhao W., Cancer Genome Project .., Leung S.Y. and Yuen S.T., BRAF and KRAS mutations in colorectal hyperplastic polyps and serrated adenomas, Cancer Research. 2003, 63: 4878-4881.

Zhao W., Chan T.L., Chu K.M., Chan A.S.Y., Stratton M.R., Yuen S.T. and Leung S.Y., Mutations of BRAF and KRAS in gastric cancer and their association with microsatellite instability, International Journal of Cancer. 2004, 108(1): 167-169.

Researcher : Zhu H

List of Research Outputs

Zhu H., Wong M.P., Lam C.L., Cai W.W., Chau W.S., Wang E., Lam W.K., Chiu S.W. and Chung L.P., Genome-wide screening of chromosomal imbalance in NSCLC by array-based comparative genomic hybridization (array-CGH), Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004.

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