Institute of Molecular Biology

THE UNIVERSITY OF HONG KONG
Research and Scholarship 2004

INSTITUTE OF MOLECULAR BIOLOGY

Researcher : Cai K

List of Research Outputs

Cai K., Sham M.H., Zheng D.X. and Xu R., Anti-HBV drug and treatment, Chinese Journal of Medicine. 2003, 50: 744-746.

Xu R., Cai K., Zheng D.X., Ma H., Xu S. and Fan S.T., Molecular therapeutics of HBV, Current Gene Therapy. 2003, 3 (4): 341-355.

Researcher : Chan WH

List of Research Outputs

Chan W.H., Chung S.S.M. and Chung S.K., The role of poly(ADP-ribose) polymerase in cataract development, 8^th Research Postgraduate Symposium, Nov . 2003.

Researcher : Chau FLJ

List of Research Outputs

Guo H., Wu X.C., Chau F.L.J., Tam S., Chung S.K., Chung S.S.M. and Yang J.Y., Aldose reductase local deficiency in the kidney is sufficient to impair urine concertrating mechanism, The 10^th SCBA 2004 Conference. 2004.

Yang J.Y., Guo H., Wu X.C., Chau F.L.J., Tam S., Chung S.K. and Chung S.S.M., Aldose reductase deficiency in kidney collecting tubules impairs the urine concentrating mechanism, International Society of Nephrology 2004 Conference on Prevention of Progression of Rental disease. 2004.

Yang J.Y., Wu X.C., Chau F.L.J., Tam S., Oates P.J., Chung S.K. and Chung S.S.M., Blocking the polyol pathway protects diabetic mice against hypertriglyceridemia, American Diabetes Association 2004 Annual Meeting. 2004.

Researcher : Chau JFL

List of Research Outputs

Chau J.F.L., Lee M.K., Chung S.K. and Chung S.S.M., Myo-inositol is important for neuromuscular functions, Journal of Neurochemistry. 2004, 88 (Suppl. 1): 85.

Chau J.F.L., Lee M.K., Chung S.K. and Chung S.S.M., Peripheral neuropathy in mice lacking the sodium/myo-inositol co-transporter gene, The 23^rd Annual Scientific Meeting, The Hong Kong Society of Neurosciences. 2003.

Chau J.F.L., Lee M.K., Chung S.K. and Chung S.S.M., Study on the role of sodium/myo-iositol co-transporter gene on neuromuscular disorders, 8^th Research Postgraduate Symposium, Faculty of Medicine, The University of Hong Kong, Hong Kong. 2003.

Researcher : Chen AYS

List of Research Outputs

Chan K.K., Chen A.Y.S., Lui V.C.H., Tam P.K.H. and Sham M.H., Abnormal enteric ganglia development in transgenic mice with ectopic expression of mutant Sox10 in the vagal neural crest. , The 4th International Meeting on Hirschprung Disease and Related Neurocristopathies HSCR, April 2004, Genova italy. 2004.

Chen A.Y.S., Chung S.S.M. and Chung S.K., Novel non-invasive approach to study the pathogenesis of chronic diabetic neuropathy in mice, The 23^rd Annual Scientific Meething, The Hong Kong Society of Neurosciences. 2003.

Chen A.Y.S., Chung S.S.M. and Chung S.K., Novel non-invasive approach to study the pathogenesis of chronic diabetic neuropathy, 8^th Research Postgraduate Symposium, Faculty of Medicine, The University of Hong Kong, Hong Kong. 2003.

Chen A.Y.S., Chung S.S.M. and Chung S.K., Novel non-invasive approach to study the pathogenesis of chronic diabetic neuropathy, Journal of Neurochemistry. 2004, 88 (Suppl. 1): 59.

Researcher : Chen J

List of Research Outputs

Chen J., He Q., Yuen A.P.W. and Chiu J., Proteomics of buccal squamous cell carcinoma: The involvement of multiple pathways in tumorigenesis, Proteomics. 2004, 4: 2465-2475.

He Q., Chen J., Kung H., Yuen P.W. and Chiu J., Identification of tumor-associated proteins in oral tongue squamous cell carcinoma by proteomics, Proteomics. 2004, 4: 271-8.

Researcher : Cheung YT

List of Research Outputs

Cheung Y.T., Kung H. and Lin M.C., Functional characterization of human cell cycle related kinase in glioblastoma carcinogenesis, TASBMB Annual Meeting and 8^th IUBMB conference in Boston Mass. USA, Jun 12-16. 2004.

Researcher : Chiu J

Project Title:	Proteomic studies on the molecular mechanisms of arsenic actions as a carcinogen and therapeutic agent
Investigator(s):	Dr. Chiu J.F.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	05/2002
Completion Date:	10/2003

Abstract:

To determine the processes of cell transformation and apoptosis induced by arsenic in cultured cells; to investigate which signal transaction pathway that mediates arsenic-induced cell transformation and cell death.

Project Title:	Regulation of a-fetoprotein gene expression in differentiating and cancer cells
Investigator(s):	Dr. Chiu J.F.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	09/2002

Abstract:

The project attempts to: (1) Identify and characterize DAS-binding protein (DAP) that regulate a-fetoprotein expression; (2) Investigate the specific DNA binding activity and biological function of DAP. The biological function will be determined by using various mutant genes, DAP antisense sequence and transcription factor decoys; (3) Identify other genes that are regulated by the DAS cis-element through a computer-assisted analysis of the genomic sequences in GenBank; and (4) Investigate the expression of these candidate genes in F9 cells during differentiation, and in developing liver cells and hepatomas.

Project Title:	Action mechanisms of nano-sized silver particles (nanosilver) as therapeutic agent
Investigator(s):	Dr. Chiu J.F., Prof. Che C.M., Dr. He Q.Y., Prof. Tam P.K.H.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	09/2003

Abstract:

To determine antibacterial and anticancer effects of nanosilver; to determine differential protein expression of cells treated with nanosilver; to identify key elements of oxidative stress that involve in nanosilver-induced antibacterial activity and anticancer effect; to determine which signal pathways that mediates nanosilver-induced cell death.

Project Title:	Biochemical and proteomic analyses of arsenic carcinogenesis
Investigator(s):	Dr. Chiu J.F., Dr. He Q.Y., Dr. Leung S.Y.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	09/2003

Abstract:

To establish and examine the processes of in vitro carcinogenesis induced by arsenic; to identify key elements of oxidative stress that involve in arsenic-induced cell transformation by biochemical and proteomic approaches; to determine which signaling pathway that mediates arsenic-induced cell transformation by proteomic approach.

Project Title:	Biochemical and proteomic analyses of arsenic carcinogenesis
Investigator(s):	Dr. Chiu J.F., Dr. He Q.Y., Dr. Leung S.Y.
Department:	Institute of Molecular Biology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	09/2003

Abstract:

List of Research Outputs

Chen J., He Q., Yuen A.P.W. and Chiu J., Proteomics of buccal squamous cell carcinoma: The involvement of multiple pathways in tumorigenesis, Proteomics. 2004, 4: 2465-2475.

Chiu J., Proteomics and its application, Hong Kong - Shanghai International Liver Congress 2004, Hong Kong, Feb 14-17. 2004.

Chiu J., Jiao R. and He Q., Purification and Identification of DAP-II Proteins That Regulate Alpha-Fetoprotein Gene Expression In Retinoic Acid-Induced F9 Cells, Abstract of ASBMB Annual Meeting and 8th IUBMB Conference, Boston, Massachusetts, June 12-16, 2004, U.S.A. . 2004, C295.

Chiu J., Jiao R. and He Q., Purification and identification of DAP-11 proteins that regulate alpha-fetoprotein gene expression in retinoic acid-induced F9 cells, American Society of Biochemistry and Molecular Biology Annual Meeting and 8^th IUBMB Conference, Boston, MA, Jun 12-16. 2004.

Chiu J., Lau G.K., Yuen S.T. and He Q., Serum biomarkers for patients with hepatitis B virus-infected liver inflammation, AACR-NCI-EROTC International Conference on "Molecular targets and cancer therapeutics: Discovery, biology, and clinical application", Boston, MA Nov 17-21. 2003.

Cutroneo K.R. and Chiu J., Inflammation, fibrosis and carcinogenesis, Recent Research Development in Cellular Biochemistry (Gayathri, A. Ed.). 2003, 1: 143-150.

He Q., Chen J., Kung H., Yuen P.W. and Chiu J., Identification of tumor-associated proteins in oral tongue squamous cell carcinoma by proteomics, Proteomics. 2004, 4: 271-8.

He Q., Ren Y., Tam P.C. and Chiu J., Truncated a1-Antitrypsin as an Infection Biomarker for Severe Acute Respiratory Syndrome (SARS), Abstracts of The American Society for Cell Biology, 43rd Annual Meeting, December 13-17, 2003, San Francisco. 2003, 523a.

Lau T.Y., He Q., Li M. and Chiu J., A Proteomic analysis of the arsenite response in cultured lung cells, 17^th Symposium of Protein Society, Boston, MA, Jul 26-30. 2003.

Lau T.Y., He Q. and Chiu J., A proteome analysis of the arsenite response in cultured lung cells: evidence for in vitro oxidative stress-induced apoptosis, Biochemical Journal. 2004, 382: 641-650.

Lau T.Y., Li M., Xie R., He Q. and Chiu J., Arsenic induces two opposite signaling pathways promoting cell proliferation or apoptosis in cultured lung cells, Carcinogenesis. 2004, 25: 21-28.

Lau T.Y., Li M., Xie R., He Q. and Chiu J., Arsenite Induces Two Opposite Signaling Pathways that leads to Cell Proliferation or Apoptosis in Cultured Lung Cells, Proceedings of the 94th Annual Meeting of American Association for Cancer Research, Washington Convention Center, Washington, D.C., July 11-14, 2003 . 2003, 44 (2nd ed.): #6192.

Lau T.Y., Li M., Xie R., He Q. and Chiu J., Arsenite induces two opposite signaling pathways that leads to cell proliferation or apoptosis in cultured lung cells, American Association for Cancer Research Annual Meeting, Washington, D.C., Jul 11-14. 2003.

Lau T.Y., Li M., Xie R., He Q. and Chiu J., Opposed Arsenite-Induced Signaling Pathways Promote Cell Proliferation or Apoptosis In Cultured Lung Cells, Carcinogenesis. 2004, 25: 21-28.

Lau T.Y., He Q. and Chiu J., Proteomic technology and its biomedical applications, Acta Biochemica Biophysica Sinica. 2003, 35: 965-975.

Lee C.Y.L., Lee C.K.F., Xu J., He Q., Chiu J., Lee W.W.M., Luk J.M.C. and Yeung W.S.B., Identification of human oviducutal cell derived embryotrophic factor-3 (ETF-3), Symposium on Reproductive Health, Macau, Hong Kong and Inland China, Macau, May 29-31, 2004 . 2004.

Lee C.Y.L., Lee C.K.F., Xu J., He Q., Chiu J., Lee W.W.M., Luk J.M.C. and Yeung W.S.B., The embryotrophic activity of oviductal cell-derived complement C3b and iC3b, a novel function of complement protein in reproduction, The Journal of Biological Chemistry. 2004, 279(13): 12763-12768.

Ren Y., He Q., Fan J., Li Z., Zou Y., Chan H.M., Bacher M., Haab B., Chiu J., Vande Woude G. and Tam P., cDNA microarray and proteomic analysis of differential gene and protein expression in human neuroblastoma cells induced by macrophage migration inhibitory factor, British Association of Paediatric Surgeons 50^th Annual International Congress, Estoril, Portugal, Jul 15-18. 2003.

Wang Y., He Q., Che C.M. and Chiu J., Action Mechanisms of Gold (III) Porphyrin Ia , Abstract of The 8th International Symposium on Applied Bioinorganic Chemistry, 2-5 April 2004, Hong Kong. 2004, PP51.

Researcher : Chung SK

Project Title:	Schwann cell-specific inactivation of neuro-proteins, aldose reductase, to study its role in diabetic neuropathy
Investigator(s):	Dr. Chung S.K.
Department:	Institute of Molecular Biology
Source(s) of Funding:	HKUST Biotechnology Research Institute
Start Date:	09/1996

Abstract:

To develop mutant mice that lack aldose reductase (AR) in the Schwann cells specifically and see how it affects the development of diabetic peripheral neuropathy.

Project Title:	Role of endothelial and astrocytic ET-1 in the pathogenesis of diabetic retinopathy, a major cause of blindness in man
Investigator(s):	Dr. Chung S.K., Dr. Lo A.C.Y.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	11/2000

Abstract:

To use astrocytes (GFAP-ET-1) mice and endothelial cell (Tie1-ET-1) mice to study the involvement of endothelin-1 (ET-1) in retinal structural lesions and barrier dysfunction in experimental diabetes.

Project Title:	Characterization of endothelin-1 gene manipulated mice for ischemic stroke, a leading cause of death and disability
Investigator(s):	Dr. Chung S.K.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	09/2002

Abstract:

The project attempts to determine if the ET-1 knockout mice have more severe ischemic brain injuries. The absolute and regional cerebral blood flow, neurological deficit, infarct volume and the detailed molecular signals will be determined.

Project Title:	Molecular mechanisms of dementia associated with aging-related diseases, alzheimer's and multi-infarct
Investigator(s):	Dr. Chung S.K.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	12/2003

Abstract:

To characterize of GET, TET or ETKO mice with human Swedish mutation in APP gene; to determine amyloidogenesis and neuropathological features in ET-1/mutant APPYAC brain; to determine memory deficit and brain activity in ET-1/mutant APPYAC mice; to determine the role of infarct and mutant APP on oxidative stress and neuropathogical features; to determine efficacy of ECE inhibitor and ETA and ETB receptors antagonists for neurotoxicity.

Project Title:	Molecular mechanisms of dementia associated with aging-related diseases, alzheimer's and multi-infarct
Investigator(s):	Dr. Chung S.K.
Department:	Institute of Molecular Biology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	12/2003

Abstract:

List of Research Outputs

Chan W.H., Chung S.S.M. and Chung S.K., The role of poly(ADP-ribose) polymerase in cataract development, 8^th Research Postgraduate Symposium, Nov . 2003.

Chau J.F.L., Lee M.K., Chung S.K. and Chung S.S.M., Myo-inositol is important for neuromuscular functions, Journal of Neurochemistry. 2004, 88 (Suppl. 1): 85.

Chau J.F.L., Lee M.K., Chung S.K. and Chung S.S.M., Peripheral neuropathy in mice lacking the sodium/myo-inositol co-transporter gene, The 23^rd Annual Scientific Meeting, The Hong Kong Society of Neurosciences. 2003.

Chau J.F.L., Lee M.K., Chung S.K. and Chung S.S.M., Study on the role of sodium/myo-iositol co-transporter gene on neuromuscular disorders, 8^th Research Postgraduate Symposium, Faculty of Medicine, The University of Hong Kong, Hong Kong. 2003.

Chen A.Y.S., Chung S.S.M. and Chung S.K., Novel non-invasive approach to study the pathogenesis of chronic diabetic neuropathy in mice, The 23^rd Annual Scientific Meething, The Hong Kong Society of Neurosciences. 2003.

Chen A.Y.S., Chung S.S.M. and Chung S.K., Novel non-invasive approach to study the pathogenesis of chronic diabetic neuropathy, 8^th Research Postgraduate Symposium, Faculty of Medicine, The University of Hong Kong, Hong Kong. 2003.

Chen A.Y.S., Chung S.S.M. and Chung S.K., Novel non-invasive approach to study the pathogenesis of chronic diabetic neuropathy, Journal of Neurochemistry. 2004, 88 (Suppl. 1): 59.

Chung S.K., Characterization of endothelin-1 gene manipulated mice for ischemic stroke, a leading cause of death and disability, Frontiers in Biomedical Research, The University of Hong Kong, Hong Kong, Dec 12. 2003.

Chung S.K., Characterization of endothelin-1 gene manipulated mice for ischemic stroke, a leading cause of death and disability, Seoul National University, College of Medicine, Korea, Mar 8. 2004.

Chung S.K., Implications of the AR gene in peripheral nerve integrity and its environmental insults in diabetes, International Symposium on Diabetic Peripheral Neuropathy and NeuroDiab Meeting, Saint Malo, France, Aug 30 to Sept 2. 2003.

Chung S.K., Role of polyol pathway in diabetic neuropathy, Fukuoka, Japan, Mar 13. 2004.

Chung S.K., School of Brain Function, Workshop on Functional Genomics,(Prof. Y.S. Chan, Director), The University of Hong Kong, Chinese University of Hong Kong, HKUST and Baptist University, Apr 20-27. 2004.

Chung S.K., Symposium on "Regulation of cerebrovascular blood flow and stroke", APSN meeting, Feb 4. 2004.

Chung S.S.M., Ho C.M.E., Lam K.S.L., Lam K.S.L. and Chung S.K., Oxidative stress and diabetic complications, The 3rd International Huaxia Congress of Endocrinology, May 25 – 28, 2004, Shanghai, China. 2004.

Dan Q., Yin S., Chung S.K., Chung S.S.M. and Lam K.S.L., Interaction between the polyol pathway and non-enzymatic glycation on aortic smooth muscle cell migration and monocyte adhesion in a transgenic mouse model, 13th International Symposium on Atherosclerosis, September 28 – October 2, 2003, Kyoto, Japan. 2003.

Dan Q., Yin S., Wong L.C., Chung S.K., Chung S.S.M. and Lam K.S.L., Interaction between the polyol pathway and non-enzymatic glycation on aortic smooth muscle cell migration and monocyte adhesion in a transgenic mouse model, 15th Annual Scientific Meeting of Hong Kong Society of Endocrinology, Metabolism and Reproduction, October 2003, Hong Kon. 2003.

Dan Q., Yin S., Wong L.C., Chung S.K., Chung S.S.M. and Lam K.S.L., Interaction between the polyol pathway and non-enzymatic glycation on aortic smooth muscle cell migration and monocyte adhesion in a transgenic mouse model, 9th Medical Research Conference, Medical Science Group, The University of Hong Kong, 7-8 February 2004.

Dan Q., Yin S., Wong L.C., Chung S.K., Chung S.S.M. and Lam K.S.L., Interaction between the polyol pathway and non-enzymatic glycation on aortic smooth muscle cell mirgration and moncyte adhesion in a transgenic mouse model, XIIIth International Symposium on Atherosclerosis, Sep 28-Oct 2, 2003, Kyoto, Japan. European Atherosclerosis Society, 2003, 1P-0137.

Dan Q., Wong L.C., Cheng K.Y., Chung S.S.M., Chung S.K. and Lam K.S.L., The polyol pathway and diabetic atherosclerosis, The 3rd International Huaxia Congress of Endocrinology, May 25 – 28, 2004, Shanghai, China. 2004.

Guo H., Wu X.C., Chau F.L.J., Tam S., Chung S.K., Chung S.S.M. and Yang J.Y., Aldose reductase local deficiency in the kidney is sufficient to impair urine concertrating mechanism, The 10^th SCBA 2004 Conference. 2004.

Lam A.K.M., Ko C.B., Tam S., Knepper M.A., Morris R., Yang J.Y., Chung S.K. and Chung S.S.M., Osmotic Response Element Binding Protein (OREBP) is essential for water reabsorption in the kidney, Experimental Biology 2004, Washington, DC, USA, April 17-21. 2004.

Lam A.K.M., Ko C.B., Tam S.C.F., Yang J.Y., Chung S.K. and Chung S.S.M., Osmotic Response Element Binding Protein is essential for water reabsorption in kidney, 8^th Research Postgraduate Symposium, Hong Kong, Dec 13. 2003.

Lam K.S.L., Dan Q., Yin S., Wong L.C., Chung S.K. and Chung S.S.M., Interaction between non-enzymatic glycation and the polyol pathway on mesangial cell gene expression in an aldose reductase transgenic mouse model, The 18th International Diabetes Federation Congress, August 24-29, 2003, Paris, France. 2003.

Law J., Lee A., Mu S., Chung S.K., Dityatev A., Schachner M. and Morellini F., Decreaed anxiety, impaired place learning and increased CA1 basal excitatory synaptic transmission in mice with conditional ablation of the neural cell adhesion molecule L1, Journal of Neuroscience. 2003, 23(32): 10419-32.

Lo A.C.Y., Wu W.H., Chung S.S.M. and Chung S.K., Astrocytic endothelin-1 leads to blood-brain barrier disruption resulting in increased infarct, brain edema and more severe neurological deficits after experimental stroke, The Joint meeting - 2003 of the ISN (International Society for Neurochemistry) and the APSN (Asian-Pacific Society for Neurochemistry), Hong Kong, Aug 3-8. 2003.

Lo A.C.Y., Law L.P., Chung S.S.M. and Chung S.K., Endothelin-1 mediated signaling for increased blood-brain barrier breakdown and infarct after transient focal cerebral ischemia, Colloquium "Genomics and Proteomics", The 6^th Biennial Meeting of the APSN (Asian-Pacific Society for Neurochemistry), Feb 4-7. 2004.

Lo A.C.Y., Yaw L.P., Chung S.S.M. and Chung S.K., Over-expression of endothelin-1 in astrocytes leads to blood-brain barrier disruption, increased infarct, brain edema and more severe neurological deficits after experimental stroke, The 23^rd Scientific Meeting of the Hong Kong Society of Neurosciences, Hong Kong, People's Republic of China, Dec 9-10. 2003.

Lo A.C.Y., Yaw L.P., Chung S.S.M. and Chung S.K., Over-expression of endothelin-1 in astrocytes leads to blood-brain barrier disruption, increased infarct, brain edema and more severe neurological deficits after experimental stroke, The Eighth International Conference on Endothelin, Tsukuba, Japan, Nov 23-26. 2003.

Lo A.C.Y., Fung M.K.L., Au C.L., Chan T.S.K., Sauer B., Chung S.S.M. and Chung S.K., Transgenic mice over-expressing endothelin-1 in testis transactivated by a cre/loxP system showed decreased testicular capillary blood flow, Transgenic Research. 2004, 13: 119-134.

Yang J.Y., Guo H., Wu X.C., Chau F.L.J., Tam S., Chung S.K. and Chung S.S.M., Aldose reductase deficiency in kidney collecting tubules impairs the urine concentrating mechanism, International Society of Nephrology 2004 Conference on Prevention of Progression of Rental disease. 2004.

Yang J.Y., Wu X.C., Chau F.L.J., Tam S., Oates P.J., Chung S.K. and Chung S.S.M., Blocking the polyol pathway protects diabetic mice against hypertriglyceridemia, American Diabetes Association 2004 Annual Meeting. 2004.

Researcher : Chung SSM

Project Title:	Effects of early postnatal feeding on fatty acid metabolism
Investigator(s):	Dr. Chung S.S.M., Dr. Sheng H.P.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	11/2002

Abstract:

Both epidemiological studies and animal studies have shown that prenatal and early postnatal nutrition not only affects infant growth and development, it also predetermines future metabolism, performance adn morbidity. This study addresses the problems of nutritional environment during the sucking period on lipid metabolism and later-life obesity.

Project Title:	Mechanism of Nogo-A inhibition of axonal regeneration
Investigator(s):	Dr. Chung S.S.M., Dr. Chung S.K., Prof. Ju G.
Department:	Institute of Molecular Biology
Source(s) of Funding:	National Natural Science Foundation of China
Start Date:	01/2003

Abstract:

To develop Nogo gene knockout mice to determine the role of this gene in the inhibition of axonal regeneration and its physiological functions; to develop transgenic mice that overexpress Nogo-A in their Schwann cells to determine if that would make their PNS non-permissive for axonal regeneraton; to develop transgenic mice that overexpress mutant Nogo-A with the Nogo-66 or aa623-640 region deleted to determine which domain is responsible for the inhibitory effect of Nogo-A.

Project Title:	Polyol pathway in diabetic dyslipidemia
Investigator(s):	Dr. Chung S.S.M.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	01/2003

Abstract:

To determine if AR null mutation reduces plasma TG levels in diabetic mice; to determine if ARI reduces plasma TG in diabetic mice; to determine if AR null mutation or ARI nor ARI normalizes LDL and HDL leviabetic mice.

Project Title:	Aldose reductase in diabetic cataract
Investigator(s):	Dr. Chung S.S.M.
Department:	Institute of Molecular Biology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	07/2003

Abstract:

In the slow-developing diabetic cataract, chronic AR-induced oxidative stress impairs the lens' osmoregulatory machinery such that lens can no longer accommodate modest increase in sorbitol, leading to cataract development; We planned to test this model by determing if AR-induced osmotic stress and oxidative stress are both important for the pathogenesis of the slow developing cataract; to determine if osmotic stress and oxidative stress act synergistically to cause cataract development ; to investigate how the impairment in the epithelial cells affects the protein aggregation in the underlying fiber cells to cause lens opacity; to characterize the changes in the epithelial cells during cataract development.

List of Research Outputs

Chan W.H., Chung S.S.M. and Chung S.K., The role of poly(ADP-ribose) polymerase in cataract development, 8^th Research Postgraduate Symposium, Nov . 2003.

Chau J.F.L., Lee M.K., Chung S.K. and Chung S.S.M., Myo-inositol is important for neuromuscular functions, Journal of Neurochemistry. 2004, 88 (Suppl. 1): 85.

Chau J.F.L., Lee M.K., Chung S.K. and Chung S.S.M., Peripheral neuropathy in mice lacking the sodium/myo-inositol co-transporter gene, The 23^rd Annual Scientific Meeting, The Hong Kong Society of Neurosciences. 2003.

Chau J.F.L., Lee M.K., Chung S.K. and Chung S.S.M., Study on the role of sodium/myo-iositol co-transporter gene on neuromuscular disorders, 8^th Research Postgraduate Symposium, Faculty of Medicine, The University of Hong Kong, Hong Kong. 2003.

Chen A.Y.S., Chung S.S.M. and Chung S.K., Novel non-invasive approach to study the pathogenesis of chronic diabetic neuropathy in mice, The 23^rd Annual Scientific Meething, The Hong Kong Society of Neurosciences. 2003.

Chen A.Y.S., Chung S.S.M. and Chung S.K., Novel non-invasive approach to study the pathogenesis of chronic diabetic neuropathy, 8^th Research Postgraduate Symposium, Faculty of Medicine, The University of Hong Kong, Hong Kong. 2003.

Chen A.Y.S., Chung S.S.M. and Chung S.K., Novel non-invasive approach to study the pathogenesis of chronic diabetic neuropathy, Journal of Neurochemistry. 2004, 88 (Suppl. 1): 59.

Chung S.S.M., Ho C.M.E., Lam K.S.L., Lam K.S.L. and Chung S.K., Oxidative stress and diabetic complications, The 3rd International Huaxia Congress of Endocrinology, May 25 – 28, 2004, Shanghai, China. 2004.

Dan Q., Yin S., Chung S.K., Chung S.S.M. and Lam K.S.L., Interaction between the polyol pathway and non-enzymatic glycation on aortic smooth muscle cell migration and monocyte adhesion in a transgenic mouse model, 13th International Symposium on Atherosclerosis, September 28 – October 2, 2003, Kyoto, Japan. 2003.

Dan Q., Yin S., Wong L.C., Chung S.K., Chung S.S.M. and Lam K.S.L., Interaction between the polyol pathway and non-enzymatic glycation on aortic smooth muscle cell migration and monocyte adhesion in a transgenic mouse model, 15th Annual Scientific Meeting of Hong Kong Society of Endocrinology, Metabolism and Reproduction, October 2003, Hong Kon. 2003.

Dan Q., Yin S., Wong L.C., Chung S.K., Chung S.S.M. and Lam K.S.L., Interaction between the polyol pathway and non-enzymatic glycation on aortic smooth muscle cell migration and monocyte adhesion in a transgenic mouse model, 9th Medical Research Conference, Medical Science Group, The University of Hong Kong, 7-8 February 2004.

Dan Q., Yin S., Wong L.C., Chung S.K., Chung S.S.M. and Lam K.S.L., Interaction between the polyol pathway and non-enzymatic glycation on aortic smooth muscle cell mirgration and moncyte adhesion in a transgenic mouse model, XIIIth International Symposium on Atherosclerosis, Sep 28-Oct 2, 2003, Kyoto, Japan. European Atherosclerosis Society, 2003, 1P-0137.

Dan Q., Wong L.C., Cheng K.Y., Chung S.S.M., Chung S.K. and Lam K.S.L., The polyol pathway and diabetic atherosclerosis, The 3rd International Huaxia Congress of Endocrinology, May 25 – 28, 2004, Shanghai, China. 2004.

Guo H., Wu X.C., Chau F.L.J., Tam S., Chung S.K., Chung S.S.M. and Yang J.Y., Aldose reductase local deficiency in the kidney is sufficient to impair urine concertrating mechanism, The 10^th SCBA 2004 Conference. 2004.

Lam A.K.M., Ko C.B., Tam S., Knepper M.A., Morris R., Yang J.Y., Chung S.K. and Chung S.S.M., Osmotic Response Element Binding Protein (OREBP) is essential for water reabsorption in the kidney, Experimental Biology 2004, Washington, DC, USA, April 17-21. 2004.

Lam A.K.M., Ko C.B., Tam S.C.F., Yang J.Y., Chung S.K. and Chung S.S.M., Osmotic Response Element Binding Protein is essential for water reabsorption in kidney, 8^th Research Postgraduate Symposium, Hong Kong, Dec 13. 2003.

Lam K.S.L., Dan Q., Yin S., Wong L.C., Chung S.K. and Chung S.S.M., Interaction between non-enzymatic glycation and the polyol pathway on mesangial cell gene expression in an aldose reductase transgenic mouse model, The 18th International Diabetes Federation Congress, August 24-29, 2003, Paris, France. 2003.

Lam T.C., Cheung B.M.Y., Ko B.C.K., Chung S.S.M. and Lau C.P., Genetic linkage of beta and gamma subunits of epithelial sodium channel to systolic blood pressure in southern Chinese, Hong Kong Medical Journal. 2003, 9(1) Suppl: 25.

Li Z., Liao C., Ko C.B., Shan S., Tong E.H., Yin Z., Pan D., Wong V.K., Shi L., Ning Z.Q., Hu W., Zhou J., Chung S.S.M. and Lu X.P., Design, synthesis, and evaluation of a new class of noncyclic 1,3-dicarbonyl compounds as PPARalpha selective activators, Bioorganic Medicinal Chemistry Letters. 2004, 14: 3507-11.

Lo A.C.Y., Wu W.H., Chung S.S.M. and Chung S.K., Astrocytic endothelin-1 leads to blood-brain barrier disruption resulting in increased infarct, brain edema and more severe neurological deficits after experimental stroke, The Joint meeting - 2003 of the ISN (International Society for Neurochemistry) and the APSN (Asian-Pacific Society for Neurochemistry), Hong Kong, Aug 3-8. 2003.

Lo A.C.Y., Law L.P., Chung S.S.M. and Chung S.K., Endothelin-1 mediated signaling for increased blood-brain barrier breakdown and infarct after transient focal cerebral ischemia, Colloquium "Genomics and Proteomics", The 6^th Biennial Meeting of the APSN (Asian-Pacific Society for Neurochemistry), Feb 4-7. 2004.

Lo A.C.Y., Yaw L.P., Chung S.S.M. and Chung S.K., Over-expression of endothelin-1 in astrocytes leads to blood-brain barrier disruption, increased infarct, brain edema and more severe neurological deficits after experimental stroke, The 23^rd Scientific Meeting of the Hong Kong Society of Neurosciences, Hong Kong, People's Republic of China, Dec 9-10. 2003.

Lo A.C.Y., Yaw L.P., Chung S.S.M. and Chung S.K., Over-expression of endothelin-1 in astrocytes leads to blood-brain barrier disruption, increased infarct, brain edema and more severe neurological deficits after experimental stroke, The Eighth International Conference on Endothelin, Tsukuba, Japan, Nov 23-26. 2003.

Lo A.C.Y., Fung M.K.L., Au C.L., Chan T.S.K., Sauer B., Chung S.S.M. and Chung S.K., Transgenic mice over-expressing endothelin-1 in testis transactivated by a cre/loxP system showed decreased testicular capillary blood flow, Transgenic Research. 2004, 13: 119-134.

Yang J.Y., Guo H., Wu X.C., Chau F.L.J., Tam S., Chung S.K. and Chung S.S.M., Aldose reductase deficiency in kidney collecting tubules impairs the urine concentrating mechanism, International Society of Nephrology 2004 Conference on Prevention of Progression of Rental disease. 2004.

Yang J.Y., Wu X.C., Chau F.L.J., Tam S., Oates P.J., Chung S.K. and Chung S.S.M., Blocking the polyol pathway protects diabetic mice against hypertriglyceridemia, American Diabetes Association 2004 Annual Meeting. 2004.

Zeindl-Eberhart E., Haraida S., Liebmann S., Jungblut P.R., Lamer S., Mayer D., Jager G., Chung S.S.M. and Rabes H.M., Detection and identification of tumor-associated protein variants in human hepatocellular carcinomas, Hepatology. 2004, 39: 540.

Researcher : Guo Y

List of Research Outputs

Peng Y., Yang P., Guo Y., Ng S.M., Liu J., Fung P.C.W., Tay D.K.C., Ge J., He M.L., Kung H. and Lin M.C., Catalase and peroxiredoxin 5 protect xenopus embryos against alcohol-induced ocular anomalies, Investigative Ophthalmology & Visual Science. 2004, 45 No. 1: 23-29.

Researcher : He ML

Project Title:	Implementation of theoretical and technical system for disease genomics
Investigator(s):	Dr. He M.L.
Department:	Institute of Molecular Biology
Source(s) of Funding:	PVC(R)'s China Collaboration Budget
Start Date:	08/2001

Abstract:

To study implementation of theoretical and technical system for disease genomics.

Project Title:	Anti-HBV gene therapy using recombinant siRNA-rAAV virus
Investigator(s):	Dr. He M.L.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	06/2003

Abstract:

To construct rAAV plasmid; to package and purify rAAV virus; to test the efficacy of anti-HBV replication in vitro; to test the synergic effects of siRNAs and lamuvidine in vitro.

Project Title:	Silencing the SARS - co virus by RNA interference
Investigator(s):	Dr. He M.L.
Department:	Institute of Molecular Biology
Source(s) of Funding:	SARS Research Fund
Start Date:	07/2003

Abstract:

To design more siRNA to knock-down the four structural genes (S,E,M,N) and test their effects in the inhibion and replication in vitro in the monkey kidney (FRhk-4 cells) cell culture system; to test the potential combinational synergistic anti-viral effects of different siRNA; to test the potential synergistic effect of siRNA in combination with other known therapeutic agents include interferon-b.

Project Title:	Transcriptional and functional regulation of TBX3 in retina formation
Investigator(s):	Dr. He M.L., Prof. Kung H.F., Dr. Peng Y., Prof. Rao Y.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	12/2003

Abstract:

To characterize TBX3 promoter and identify elements that are responsible for the inhibition of retina formation; to characterize whether TBX3 has common and distinct function with TBX2 in retina formation; to elucidate the roles and relationship with other molecules (BMP4, jun/fos, E2F1) in the BMP4-pRB-p53 signaling pathway.

Project Title:	Transcriptional and functional regulation of TBX3 in retina formation
Investigator(s):	Dr. He M.L., Prof. Kung H.F., Dr. Peng Y., Prof. Rao Y.
Department:	Institute of Molecular Biology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	12/2003

Abstract:

Project Title:	Development of lentiviral vectors for gene therapy
Investigator(s):	Dr. He M.L.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	06/2004

Abstract:

To construct pLenti expression vector; to package and purify Lentiviruses; to test transduction efficiency; to test in vitro anti-HBV efficacy.

Project Title:	Gene therapy for the treatment of HBV infection, HBV-induced liver cancer
Investigator(s):	Dr. He M.L., Prof. Kung H.F., Dr. Lin M.C.M., Prof. Yuen K.Y., Dr. Zheng B.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Innovation and Technology Fund, Innovation and Technology Commission of Hong Kong Government
Start Date:	07/2004

Abstract:

To develop an innovative and effective gene therapies to treat HBV infection and HCC.

List of Research Outputs

Chen Y., Du D., Wu J., Chan C.P., Tan Y.Q., Kung H. and He M.L., Inhibition of hepatitis B virus (HBV) replication by stably-expressed shRNA, Biochemical and Biophysical Research Communications. 2003, 311: 398-404.

Chen Y., Sze J. and He M.L., The occurrence of HBV cccDNA in the patients' sera is an indicator for HBV reactivation and an early signal of liver damage, World Journal of Gastroenterology. 2004, 10: 82-85.

He M.L., Kung H. and Lin M.C., A new and sensitive method for the quantification of HBV cccDNA by real time PCR, 2003 US Provisional patent no. 60/383, 953, US Non-Provisional regular patent filed March 25, 2004 . 2004.

He M.L., Catalase and peroxiredoxin 5 protect xenopus embryos against alcohol-induced ocular anomalies, The American Society for Cell Biology 43^rd Annual Meeting, San Francisco, CA, USA, Dec 13-17. 2003.

He M.L., HBV cccDNA in patients' sera as an indicator for HBV reactivation and an early signal of liver damage, Hong Kong-Shanghai International Liver Congress, Hong Kong, China, Feb. 2004.

He M.L., Inhibition of HBV replication by long-term expressed interferon-alpha vs reecombinant adeno-associated virus mediated gene transferring, Hong Kong-Shanghai International Liver Congress, Hong Kong, China, Feb. 2004.

He M.L., Peng Y., Kung H. and Lin M.C., Inhibition of SARS-associated coronavirus infection and replication by RNA interference, 2003 US provisional patent No. 60/471, 922. 2003.

He M.L., Zheng B., Peng Y., Peiris J.S., Poon L.L., Yuen K.Y., Lin M.C., Kung H. and Guan Y., Inhibition of SARS-associated coronavirus infection and replication by RNA interference, Journal of the American Medical Association. 2003, 290(20): 2665-6.

He M.L., Inhibition of SARS-associated coronavirus infection and replication by siRNA, The American Society for Cell Biology 43^rd Annual Meeting, San Francisco, CA, USA, Dec 13-17. 2003.

He M.L., Inhibition of hepatitis B virus replication by stably expressed shRNA, Hong Kong-Shanghai International Liver Congress, Hong Kong, China, Feb. 2004.

He M.L., Silence of HCAO gene expression by RNA interference led to an elevated TERT expression in HCC cells, Hong Kong-Shanghai International Liver Congress, Hong Kong, China, Feb. 2004.

He M.L., The mechanisms of a novel HCC-associated oncogene (HCAO) in hepatocellular carcinogenesis, Hong Kong-Shanghai International Liver Congress, Hong Kong, China, Feb. 2004.

Peng Y., Yang P., Ng S.M., Wong O.G., Liu J., He M.L., Kung H. and Lin M.C., A critical role of Pax6 in alcohol-induced fetal microcephaly, Neurobiology of Disease. 2004, 16: 370-376.

Peng Y., Yang P., Guo Y., Ng S.M., Liu J., Fung P.C.W., Tay D.K.C., Ge J., He M.L., Kung H. and Lin M.C., Catalase and peroxiredoxin 5 protect xenopus embryos against alcohol-induced ocular anomalies, Investigative Ophthalmology & Visual Science. 2004, 45 No. 1: 23-29.

Zheng B., Guan Y., Tang Q., Cheng D., Xie F.Y., He M.L., Chan K.W., Wong K.L., Lader E., Woodle M.C., Lu P.Y., Li B. and Zhong N., Prophylactic and therapeutic effects of small interfering RNA targeting SARS-coronavirus, Antiviral Therapy. 2004, 9: 365-374.

Researcher : Jiao R

List of Research Outputs

Chiu J., Jiao R. and He Q., Purification and Identification of DAP-II Proteins That Regulate Alpha-Fetoprotein Gene Expression In Retinoic Acid-Induced F9 Cells, Abstract of ASBMB Annual Meeting and 8th IUBMB Conference, Boston, Massachusetts, June 12-16, 2004, U.S.A. . 2004, C295.

Researcher : Kung H

Project Title:	Molecular basis of MAD1 mitotic checkpoint functions in mammalian cells
Investigator(s):	Prof. Kung H.F., Dr. Jin D.Y.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	09/2000

Abstract:

Cell cycle checkpoints in eukaryotes are highly conserved regulatory pathways that ensure the orderly and faithful progression of critical events. Loss of checkpoint results in genetic instability, which is a hallmark of cancers. The project aims to investigate the molecular basis of MAD1 (a central component of the mitotic checkpoint) functions in mammalian cells.

Project Title:	Basic research on systemic damage in early stage and wound-healing after severe trauma
Investigator(s):	Prof. Kung H.F.
Department:	Institute of Molecular Biology
Source(s) of Funding:	PVC(R)'s China Collaboration Budget
Start Date:	04/2001

Abstract:

To study basic research on systemic damage in early stage and wound-healing after severe trauma.

Project Title:	Study of a novel pathway that links small G protein to neuronal differentiation - Regulation of the neuronal cdc2-like kinase by chimaerin
Investigator(s):	Prof. Kung H.F., Dr. Ching Y.P.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	12/2001

Abstract:

To understand the regulation of Nclk; to understand the regulation of actin reorganization in neuron during neuronal differentiation and neurite outgrowth; to study if Nclk regulates the neuronal cell death via modulation of PAK activity and to study whether this apoptotic phenomenon is linked to the pathogenesis of AD.

Project Title:	Molecular basis for the telomere dysfunction and anti-tumor activities of a C-terminal polypeptide of human telomerase reverse transcriptase (hTERTC27)
Investigator(s):	Prof. Kung H.F., Dr. Lin M.C.M., Dr. Wong B.C.Y.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	12/2003

Abstract:

To identify the proteins which interact with hTERTC27; to charaterize the anti-tumor activity of hTERTC27 in vitro using varied TERT positive and TERT negative cell lines and stem cells; to test the efficacy of hTERTC27 cancer gene therapy against glioblastoma in vivo in nude mice xenograft using an Adeno Associated Virus and Adenovirus-based gene delivery system.

Project Title:	Molecular basis for the telomere dysfunction and anti-tumor activities of a C-terminal polypeptide of human telomerase reverse transcriptase (hTERTC27)
Investigator(s):	Prof. Kung H.F., Dr. Lin M.C.M., Dr. Wong B.C.Y.
Department:	Institute of Molecular Biology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	12/2003

Abstract:

Project Title:	Development of a novel insect cell system for large scale recombinant AAV production
Investigator(s):	Prof. Kung H.F., Dr. He M.L., Dr. Lin M.C.M.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	01/2004

Abstract:

To develop a robust, cost-effective, large-scale system for the preparation of high-titer, high-purity recombinant AAV vector sing the baculovirus and insect Sf9 cells.

Project Title:	Functional characterization of Makorin-2 and its interacting proteins in Xenopus embryonic development
Investigator(s):	Prof. Kung H.F., Prof. Chen Z., Dr. He M.L., Dr. Huang Q.H., Dr. Lin M.C.M., Dr. Peng Y., Dr. Zhang Q.H.
Department:	Institute of Molecular Biology
Source(s) of Funding:	National Natural Science Foundation of China
Start Date:	05/2004

Abstract:

To characterize the functions of Xmakorin-2 and its interacting proteins in hematopoiesis / neurogenesis in Xenopus embryos.

Project Title:	Functional characterization of Makorin-2 and its interacting proteins in Xenopus embryonic development
Investigator(s):	Prof. Kung H.F., Prof. Chen Z., Dr. He M.L., Dr. Huang Q.H., Dr. Lin M.C.M., Dr. Peng Y., Dr. Zhang Q.H.
Department:	Institute of Molecular Biology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	05/2004

Abstract:

To characterize the functions of Xmakorin-2 and its interacting proteins in hematopoiesis / neurogenesis in Xenopus embryos.

Project Title:	Research and development of a new TCM-based antidepressant
Investigator(s):	Prof. Kung H.F.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Hong Kong Jockey Club Institute of Chinese Medicine Ltd.
Start Date:	06/2004

Abstract:

To obtain solid data for the application of new TCM drug for clinical trial in accordance with the National New Drug Application in China which will lead to the issuance of license for clinical trial.

Project Title:	Development of Novel AAV based anti-angiogenesis gene therapy for the treatment of liver cancer
Investigator(s):	Prof. Kung H.F., Prof. Fan S.T., Prof. Gan R.B., Dr. He M.L., Dr. Lin M.C.M., Dr. Ng S.S.M., Dr. Wong B.C.Y.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Innovation and Technology Fund, Innovation and Technology Commission of Hong Kong Government
Start Date:	08/2004

Abstract:

To develop an innovative and effective gene therapies to treat hepatocellular carcinoma (HCC).

List of Research Outputs

Chen Y., Luk K.D.K., Cheung K.M.C., Lu W.W., An X., Ng S.M., Lin M.C. and Kung H., Combination of adeno-associated virus and adenovirus vectors expressing bone morphogenetic protein-2 produces enhanced osteogenic activity in immunocompetent rats, Biochemical and Biophysical Research Communications. 2004, 317(3): 675-681.

Chen Y., Luk K.D.K., Cheung K.M., Xu R., Lin M.C., Lu W.W., Leong J.C. and Kung H., Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vectors, Gene Therapy. 2003, 10(16): 1345-53.

Chen Y., Du D., Wu J., Chan C.P., Tan Y.Q., Kung H. and He M.L., Inhibition of hepatitis B virus (HBV) replication by stably-expressed shRNA, Biochemical and Biophysical Research Communications. 2003, 311: 398-404.

Cheung K.M.C., Chen Y., Luk K.D.K., Xu R., Lin M.C., Lu W.W., Leong J.C.Y. and Kung H., Delivery of bone morphogenetic proteins 2 anad 4 by use of adeno-associated viral gene therapy, Hong Kong Orthopaedic Association, Hong Kong, November 8-9, 2003.

Cheung K.M.C., Chen Y., Luk K.D.K., Kung H. and Xu R., Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vectors, 38th Annual Meeting of the Scoliosis Research Society, Quebec, Canada, September 10-13, 2003.

Cheung Y.T., Kung H. and Lin M.C., Functional characterization of human cell cycle related kinase in glioblastoma carcinogenesis, TASBMB Annual Meeting and 8^th IUBMB conference in Boston Mass. USA, Jun 12-16. 2004.

Ching Y.P., Leong V.Y.L., Wong C.M. and Kung H., Identification of an autoinhibitory domain of p21-activated protein kinase 5, Journal of Biological Chemistry. 2003, 278(36): 33621-33624.

He M.L., Kung H. and Lin M.C., A new and sensitive method for the quantification of HBV cccDNA by real time PCR, 2003 US Provisional patent no. 60/383, 953, US Non-Provisional regular patent filed March 25, 2004 . 2004.

He M.L., Peng Y., Kung H. and Lin M.C., Inhibition of SARS-associated coronavirus infection and replication by RNA interference, 2003 US provisional patent No. 60/471, 922. 2003.

He M.L., Zheng B., Peng Y., Peiris J.S., Poon L.L., Yuen K.Y., Lin M.C., Kung H. and Guan Y., Inhibition of SARS-associated coronavirus infection and replication by RNA interference, Journal of the American Medical Association. 2003, 290(20): 2665-6.

He Q., Chen J., Kung H., Yuen P.W. and Chiu J., Identification of tumor-associated proteins in oral tongue squamous cell carcinoma by proteomics, Proteomics. 2004, 4: 271-8.

Kung H., Chen Y., Luk K.D.K. and Lin M.C., Combined adeno-associated virus and adenovirus cocktail gene delivery system for high efficiency gene expression without eliciting immune response in immuno-competent subjects, 17 March 2003 US Provisional patent no. 60/455, 188, US Non-Provisional patent filed March 2004. 2004.

Kung H. and Xu R., Long term expression of angiostatin and suppression of liver metastatic cancer, Provisional patent no. 9661/048/888. 2004.

Leung W.L., Chan D., Kung H. and Cheah K.S.E., The role/s of type IIA procollagen in BMP (Bone morphogenetic protein) Signalling, 8th Postgraduate Symposium, HKU, December 2003 Hong Kong . 2003.

Leung W.L., Wong S.Y.Y., Chan D., Kung H. and Cheah K.S.E., The role/s of type IIA procollagen in BMP (Bone morphogenetic protein) signaling., 2004 Sir Edward youde Memorial Fund postgraduate Conference on Model Organism Research and Human Diseases, 14-15 June 2004 Hong Kong.. 2004.

Li G., Xia H.H.X., Chen M.H., Peng J., Gu Q., Cui J., Cho C.H., Lam S.K., Lin M.C., Berg D.E., Feng Z., Langenbach R., Kung H. and Wong B.C.Y., Cyclooxygenase-2 Gene Disruption Enhances Gastric Inflammation but Inhibits Gastric Epithelial Proliferation Induced by H. pylori Infection, Gastroenterology. 2004, 126 (4 Suppl 2): W907.

Li X., Li G., Peng Y., Kung H. and Lin M.C., Suppression of Epstein-Barr virus-encoded latent membrane protein-1 by RNA interference inhibits the metastatic potential of nasopharyngeal carcinoma cells, Biochemical and Biophysical Research Communications. 2004, 315(1): 212-8.

Lin M.C., Peng Y. and Kung H., Catalase and peroxiredoxin 5 protect xenopus embryos against alcohol-induced ocular anomalies, 43^rd American Society for Cell Biology Annual Meeting, San Fancisco, CA, Dec 13-17. 2003.

Luk K.D.K., Chen Y., Cheung K.M.C., Kung H., Lu W.W. and Leong J.C.Y., Adeno-associated virus-mediated bone morphogenetic protein-4 gene therapy for in vivo bone formation, Biochemical and Biophysical Research Communications. 2003, 308: 636-645.

Luk K.D.K., Chen Y. and Kung H., Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vector, 23rd ASEAN Orthopaedic Association Congress, Kota Kinabalu, Malaysia, October 2-5, 2003.

Peng Y., Yang P., Ng S.M., Wong O.G., Liu J., He M.L., Kung H. and Lin M.C., A critical role of Pax6 in alcohol-induced fetal microcephaly, Neurobiology of Disease. 2004, 16: 370-376.

Peng Y., Kung H. and Lin M.C., A novel Xenopus laevis model to screen for molecular target and therapeutic drugs for alcohol-induced birth defects, including eye, brain, gut and growth retardation, US Provisional patent filed June 2004. 2004.

Peng Y., Yang P., Guo Y., Ng S.M., Liu J., Fung P.C.W., Tay D.K.C., Ge J., He M.L., Kung H. and Lin M.C., Catalase and peroxiredoxin 5 protect xenopus embryos against alcohol-induced ocular anomalies, Investigative Ophthalmology & Visual Science. 2004, 45 No. 1: 23-29.

Peng Y., Yang P., Ng S.M., Lum C.T., Kung H. and Lin M.C., Protection of Xenopus laevis embryos against alcohol-induced delayed gut maturation and growth retardation by peroxiredoxin 5 and catalase, Journal of Molecular Biology. 2004, 340: 819-827.

Qi R.Z., Ching Y.P., Kung H. and Wang J.H., a-Chimaerin exists in a functional complex with the Cdk5 kinase in brain, FEBS Letters. 2004, 561(1-3): 177-180.

Tanner J.A., Watt R.M., Chai Y.B., Lu L.Y., Lin M.C., Peiris J.S., Poon L.L., Kung H. and Huang J., The severe acute respiratory syndrome (SARS) coronavirus NTPase/helicase belongs to a distinct class of 5' to 3' viral helicases, Journal of Biological Chemistry. 2003, 278(41): 39578-82.

Tu S., Chan O.O., Lin M.C., Jiang X., Lam S.K., Kung H. and Wong B.C.Y., Livin, a Novel Member of Inhibitor of Apoptosis, Is Marker of Poor Prognosis in Gastric Cancer, Gastroenterology. 2004, 126 (4 Suppl 2): T1256.

Tu S., Jiang X., Lin M.C., Cui J., Yang Y., Lum C.T., Zou B., Zhu Y.B., Jiang S.H., Wong W.M., Chan O.O., Yuen M.F., Lam S.K., Kung H. and Wong B.C.Y., Suppression of Survivin Expression Inhibits in vivo Tumorigenicity and Angiogenesis in Gastric Cancer, Cancer Research. 2003, 63(22): 7724-7732.

Wang J., Gu Q., Xia H.H.X., Tu S., Peng J., Yang Y., Zou B., Lin M.C., Kung H., Lam S.K. and Wong B.C.Y., JNK1 Up-Regulates the Expression of XIAP-Associated Factor 1 (XAF1) by a P53-Dependent Pathway in Gastrointestinal Cancer, Gastroenterology. 2004, 126 (4 Suppl 2): M946.

Wong B.C.Y., Tu S., Kung H. and Lin M.C., A novel cocktail therapy for colon cancer: Combination of Adeno-associated virus (AAV) based survivin mutant gene therapy with chemotherapy, US Provisional patent filed June 2004. 2004.

Wong B.C.Y., Jiang X., Lin M.C., Tu S., Cui J., Jiang S.H., Wong R.W.M., Yuen M.F., Lam S.K. and Kung H., Cyclooxygenase-2 Inhibitor (SC-236) Suppresses Activator Protein-1 through c-Jun NH2-Terminal Kinase, Gastroenterology. 2004, 126: 136-147.

Xu R., Sun X., Tse L.Y., Li H., Chan P.C., Xu S., Xiao W., Kung H., Krissansen G.W. and Fan S.T., Long-term expression of angiostatin suppresses metatatic liver cancer in mice (Abstract), The 5th Annual Scientific Meeting, Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 3 January 2004.

Yang Y., Lin M.C., Ching Y.P., Lam S.K., Xia H.H.X., Tu S., Zou B., Wang J., Li G., He H., Gu Q., Peng J., Kung H. and Wong B.C.Y., FHL2 As a Co-Factor of XAF1 in the Induction of Apoptosis, Gastroenterology. 2004, 126 (4 Suppl 2): S938.

Zou B., Lin M.C., Lam S.K., Tu S., Yang Y., Wang J., He H., Peng J., Kung H. and Wong B.C.Y., Hypermethylation Status Around Exon 1 of XIAP-Associated Factor 1 in Human Gastric and Colon Cancer Cell Lines, Gastroenterology. 2004, 126 (4 Suppl 2): T981.

Researcher : Lam AKM

List of Research Outputs

Lam A.K.M., Ko C.B., Tam S., Knepper M.A., Morris R., Yang J.Y., Chung S.K. and Chung S.S.M., Osmotic Response Element Binding Protein (OREBP) is essential for water reabsorption in the kidney, Experimental Biology 2004, Washington, DC, USA, April 17-21. 2004.

Lam A.K.M., Ko C.B., Tam S.C.F., Yang J.Y., Chung S.K. and Chung S.S.M., Osmotic Response Element Binding Protein is essential for water reabsorption in kidney, 8^th Research Postgraduate Symposium, Hong Kong, Dec 13. 2003.

Researcher : Lau TY

List of Research Outputs

Lau T.Y., He Q., Li M. and Chiu J., A Proteomic analysis of the arsenite response in cultured lung cells, 17^th Symposium of Protein Society, Boston, MA, Jul 26-30. 2003.

Lau T.Y., He Q. and Chiu J., A proteome analysis of the arsenite response in cultured lung cells: evidence for in vitro oxidative stress-induced apoptosis, Biochemical Journal. 2004, 382: 641-650.

Lau T.Y., Li M., Xie R., He Q. and Chiu J., Arsenic induces two opposite signaling pathways promoting cell proliferation or apoptosis in cultured lung cells, Carcinogenesis. 2004, 25: 21-28.

Lau T.Y., Li M., Xie R., He Q. and Chiu J., Arsenite Induces Two Opposite Signaling Pathways that leads to Cell Proliferation or Apoptosis in Cultured Lung Cells, Proceedings of the 94th Annual Meeting of American Association for Cancer Research, Washington Convention Center, Washington, D.C., July 11-14, 2003 . 2003, 44 (2nd ed.): #6192.

Lau T.Y., Li M., Xie R., He Q. and Chiu J., Arsenite induces two opposite signaling pathways that leads to cell proliferation or apoptosis in cultured lung cells, American Association for Cancer Research Annual Meeting, Washington, D.C., Jul 11-14. 2003.

Lau T.Y., Li M., Xie R., He Q. and Chiu J., Opposed Arsenite-Induced Signaling Pathways Promote Cell Proliferation or Apoptosis In Cultured Lung Cells, Carcinogenesis. 2004, 25: 21-28.

Lau T.Y., He Q. and Chiu J., Proteomic technology and its biomedical applications, Acta Biochemica Biophysica Sinica. 2003, 35: 965-975.

Researcher : Lee MK

List of Research Outputs

Chau J.F.L., Lee M.K., Chung S.K. and Chung S.S.M., Myo-inositol is important for neuromuscular functions, Journal of Neurochemistry. 2004, 88 (Suppl. 1): 85.

Chau J.F.L., Lee M.K., Chung S.K. and Chung S.S.M., Peripheral neuropathy in mice lacking the sodium/myo-inositol co-transporter gene, The 23^rd Annual Scientific Meeting, The Hong Kong Society of Neurosciences. 2003.

Chau J.F.L., Lee M.K., Chung S.K. and Chung S.S.M., Study on the role of sodium/myo-iositol co-transporter gene on neuromuscular disorders, 8^th Research Postgraduate Symposium, Faculty of Medicine, The University of Hong Kong, Hong Kong. 2003.

Researcher : Leong VYL

List of Research Outputs

Ching Y.P., Leong V.Y.L., Wong C.M. and Kung H., Identification of an autoinhibitory domain of p21-activated protein kinase 5, Journal of Biological Chemistry. 2003, 278(36): 33621-33624.

Researcher : Li H

List of Research Outputs

Xu R., Sun X., Tse L.Y., Li H., Chan P.C., Xu S., Xiao W., Kung H., Krissansen G.W. and Fan S.T., Long-term expression of angiostatin suppresses metatatic liver cancer in mice (Abstract), The 5th Annual Scientific Meeting, Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 3 January 2004.

Researcher : Li X

List of Research Outputs

Li X., Li G., Peng Y., Kung H. and Lin M.C., Suppression of Epstein-Barr virus-encoded latent membrane protein-1 by RNA interference inhibits the metastatic potential of nasopharyngeal carcinoma cells, Biochemical and Biophysical Research Communications. 2004, 315(1): 212-8.

Researcher : Lin MC

Project Title:	Ethanol regulated gene transcription: identification of "ethanol-responsive-cis-element" in the promoter region of human MTP gene
Investigator(s):	Dr. Lin M.C.M., Dr. Jin D.Y.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	09/2000

Abstract:

To characterize the "Ethanol Responsive cis-Elecment" (ERE) and its interacting DNA binding proteins using human triglyceride transfer protein (MTP) promoter as the model system. The project will contribute to the understanding of the total outcome of low/moderate alcohol consumption.

Project Title:	Developmental function, molecular target, and transcriptional regulation of the cold-inducible RNA binding protein
Investigator(s):	Dr. Lin M.C.M.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	12/2001

Abstract:

To elucidate the function of XCIRP-1 in Xenopus embryonic neural development; to discover the specific down-stream target RNAs of the DCIRP-1 protein in order to understand the molecular mechanisms of the XCIRP-1 actions; to clone the promoter of human CIRP gene, characterize the transcriptional regulation, and investigate the cold- and heat-induced regulation of hCIRP gene expression in human brain- and liver-derived cell lines.

Project Title:	Basic research on the mechanism of aging and the prevention of geriatric disease
Investigator(s):	Dr. Lin M.C.M.
Department:	Institute of Molecular Biology
Source(s) of Funding:	PVC(R)'s China Collaboration Budget
Start Date:	12/2001

Abstract:

To study the mechanism of aging and the prevention of geriatric disease.

Project Title:	Characterization of a novel cell cycle related kinase in glioblastoma
Investigator(s):	Dr. Lin M.C.M., Dr. Ching Y.P., Dr. Leung S.Y.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	12/2002

Abstract:

The project aims at characterizing the role of cell cycle related kinase (CCRK) in cell cycle control, apoptosis, cell division and cell proliferation. Potential tumorigenic function of CCRK in human glioblastoma, fibroblast cell lines, and nude mice xenograft will also be investigated.

Project Title:	Functional characterization of novel cytokines and chemokines in host immune response to HBV infection
Investigator(s):	Dr. Lin M.C.M.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	01/2003
Completion Date:	12/2003

Abstract:

To determine the differential gene expression of these novel cytokines and chemokines in the peripheral blood mononuclear cells (PBMCs) in HLA-matched sibling pairs who had different outcome to HBV infection, i.e. HBV carrier Vs natural immunity against HBV infection; to test whether these genes have differentially regulated in PBMC stimulated by HBV core antigen.

Project Title:	Cancer gene therapy using a novel anti-cancer polypeptide hTERTC27 delivered by a novel AAV and Adenovirus Cocktail vector system
Investigator(s):	Dr. Lin M.C.M., Prof. Kung H.F., Dr. Peng Y., Dr. Wong B.C.Y.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Innovation and Technology Fund, Innovation and Technology Commission of Hong Kong Government
Start Date:	03/2003

Abstract:

To optimize our innovative, novel and patented hTERTC27 cancer gene therapy and the AAV/Adv cocktail vector technologies developed in our laboratory; to establish patented stable cell line and platforms for the large scale production of AAV-hTERTC27 and Adv-hTERTC27; to carry out, using the above, in pre-clinical study for treating solid tumors.

Project Title:	Isolation and characterization of the silkworm factors that enhance the intestinal absorption/transport of orally delivered therapeutic proteins
Investigator(s):	Dr. Lin M.C.M.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	05/2003

Abstract:

To isolate and characterize silkworm factors responsible for enhancing the intestinal absorption / transport of orally delivered protein drugs.

Project Title:	Molecular basis of alcohol-induced birth defects, the critical role of Pax 6
Investigator(s):	Dr. Lin M.C.M., Dr. Peng Y., Dr. Wong B.C.Y., Dr. Yang J.Y.Q.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	09/2003

Abstract:

To elucidate the signaling pathways leading to alcohol-mediated inhibition of Pax6 expression and to identify the targets downstream of Pax6 responsible for microcephaly. We are particularly interested in the involvements of the PI3 kinase pathways and the reactive oxygen species and reactive nitrogen species; to investigate the molecular mechanisms for alcohol-induced growth retardation, in particular its relationship with gut development; to study alcohol induced eye deformation; to screen for agents that protect against alcohol induced birth defects.

Project Title:	Molecular basis of alcohol-induced birth defects, the critical role of Pax 6
Investigator(s):	Dr. Lin M.C.M., Dr. Peng Y., Dr. Wong B.C.Y., Dr. Yang J.Y.Q.
Department:	Institute of Molecular Biology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	09/2003

Abstract:

List of Research Outputs

Chen Y., Luk K.D.K., Cheung K.M.C., Lu W.W., An X., Ng S.M., Lin M.C. and Kung H., Combination of adeno-associated virus and adenovirus vectors expressing bone morphogenetic protein-2 produces enhanced osteogenic activity in immunocompetent rats, Biochemical and Biophysical Research Communications. 2004, 317(3): 675-681.

Chen Y., Luk K.D.K., Cheung K.M., Xu R., Lin M.C., Lu W.W., Leong J.C. and Kung H., Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vectors, Gene Therapy. 2003, 10(16): 1345-53.

Cheung K.M.C., Chen Y., Luk K.D.K., Xu R., Lin M.C., Lu W.W., Leong J.C.Y. and Kung H., Delivery of bone morphogenetic proteins 2 anad 4 by use of adeno-associated viral gene therapy, Hong Kong Orthopaedic Association, Hong Kong, November 8-9, 2003.

Cheung Y.T., Kung H. and Lin M.C., Functional characterization of human cell cycle related kinase in glioblastoma carcinogenesis, TASBMB Annual Meeting and 8^th IUBMB conference in Boston Mass. USA, Jun 12-16. 2004.

He M.L., Kung H. and Lin M.C., A new and sensitive method for the quantification of HBV cccDNA by real time PCR, 2003 US Provisional patent no. 60/383, 953, US Non-Provisional regular patent filed March 25, 2004 . 2004.

He M.L., Peng Y., Kung H. and Lin M.C., Inhibition of SARS-associated coronavirus infection and replication by RNA interference, 2003 US provisional patent No. 60/471, 922. 2003.

He M.L., Zheng B., Peng Y., Peiris J.S., Poon L.L., Yuen K.Y., Lin M.C., Kung H. and Guan Y., Inhibition of SARS-associated coronavirus infection and replication by RNA interference, Journal of the American Medical Association. 2003, 290(20): 2665-6.

Kung H., Chen Y., Luk K.D.K. and Lin M.C., Combined adeno-associated virus and adenovirus cocktail gene delivery system for high efficiency gene expression without eliciting immune response in immuno-competent subjects, 17 March 2003 US Provisional patent no. 60/455, 188, US Non-Provisional patent filed March 2004. 2004.

Li G., Xia H.H.X., Chen M.H., Peng J., Gu Q., Cui J., Cho C.H., Lam S.K., Lin M.C., Berg D.E., Feng Z., Langenbach R., Kung H. and Wong B.C.Y., Cyclooxygenase-2 Gene Disruption Enhances Gastric Inflammation but Inhibits Gastric Epithelial Proliferation Induced by H. pylori Infection, Gastroenterology. 2004, 126 (4 Suppl 2): W907.

Li X., Li G., Peng Y., Kung H. and Lin M.C., Suppression of Epstein-Barr virus-encoded latent membrane protein-1 by RNA interference inhibits the metastatic potential of nasopharyngeal carcinoma cells, Biochemical and Biophysical Research Communications. 2004, 315(1): 212-8.

Lin M.C., Peng Y. and Kung H., Catalase and peroxiredoxin 5 protect xenopus embryos against alcohol-induced ocular anomalies, 43^rd American Society for Cell Biology Annual Meeting, San Fancisco, CA, Dec 13-17. 2003.

Lin M.C., Development of novel therapies for liver cancer, 2^nd China-Japan Symposium on Liver Cancer, Hong Kong, Feb 14-17. 2004.

Lin M.C., The adeno-associated virus plus adenovirus cocktail vector system produces high gene transduction efficiency and low immuno-response in rats, The forum of quality standard, control technology and safety evaluation of gene therapy products, Beijing, China, May. 2004.

Peng Y., Yang P., Ng S.M., Wong O.G., Liu J., He M.L., Kung H. and Lin M.C., A critical role of Pax6 in alcohol-induced fetal microcephaly, Neurobiology of Disease. 2004, 16: 370-376.

Peng Y., Kung H. and Lin M.C., A novel Xenopus laevis model to screen for molecular target and therapeutic drugs for alcohol-induced birth defects, including eye, brain, gut and growth retardation, US Provisional patent filed June 2004. 2004.

Peng Y., Yang P., Guo Y., Ng S.M., Liu J., Fung P.C.W., Tay D.K.C., Ge J., He M.L., Kung H. and Lin M.C., Catalase and peroxiredoxin 5 protect xenopus embryos against alcohol-induced ocular anomalies, Investigative Ophthalmology & Visual Science. 2004, 45 No. 1: 23-29.

Peng Y., Yang P., Ng S.M., Lum C.T., Kung H. and Lin M.C., Protection of Xenopus laevis embryos against alcohol-induced delayed gut maturation and growth retardation by peroxiredoxin 5 and catalase, Journal of Molecular Biology. 2004, 340: 819-827.

Tanner J.A., Watt R.M., Chai Y.B., Lu L.Y., Lin M.C., Peiris J.S., Poon L.L., Kung H. and Huang J., The severe acute respiratory syndrome (SARS) coronavirus NTPase/helicase belongs to a distinct class of 5' to 3' viral helicases, Journal of Biological Chemistry. 2003, 278(41): 39578-82.

Tu S., Chan O.O., Lin M.C., Jiang X., Lam S.K., Kung H. and Wong B.C.Y., Livin, a Novel Member of Inhibitor of Apoptosis, Is Marker of Poor Prognosis in Gastric Cancer, Gastroenterology. 2004, 126 (4 Suppl 2): T1256.

Tu S., Jiang X., Lin M.C., Cui J., Yang Y., Lum C.T., Zou B., Zhu Y.B., Jiang S.H., Wong W.M., Chan O.O., Yuen M.F., Lam S.K., Kung H. and Wong B.C.Y., Suppression of Survivin Expression Inhibits in vivo Tumorigenicity and Angiogenesis in Gastric Cancer, Cancer Research. 2003, 63(22): 7724-7732.

Wang J., Gu Q., Xia H.H.X., Tu S., Peng J., Yang Y., Zou B., Lin M.C., Kung H., Lam S.K. and Wong B.C.Y., JNK1 Up-Regulates the Expression of XIAP-Associated Factor 1 (XAF1) by a P53-Dependent Pathway in Gastrointestinal Cancer, Gastroenterology. 2004, 126 (4 Suppl 2): M946.

Wong B.C.Y., Tu S., Kung H. and Lin M.C., A novel cocktail therapy for colon cancer: Combination of Adeno-associated virus (AAV) based survivin mutant gene therapy with chemotherapy, US Provisional patent filed June 2004. 2004.

Wong B.C.Y., Jiang X., Lin M.C., Tu S., Cui J., Jiang S.H., Wong R.W.M., Yuen M.F., Lam S.K. and Kung H., Cyclooxygenase-2 Inhibitor (SC-236) Suppresses Activator Protein-1 through c-Jun NH2-Terminal Kinase, Gastroenterology. 2004, 126: 136-147.

Yang Y., Lin M.C., Ching Y.P., Lam S.K., Xia H.H.X., Tu S., Zou B., Wang J., Li G., He H., Gu Q., Peng J., Kung H. and Wong B.C.Y., FHL2 As a Co-Factor of XAF1 in the Induction of Apoptosis, Gastroenterology. 2004, 126 (4 Suppl 2): S938.

Zou B., Lin M.C., Lam S.K., Tu S., Yang Y., Wang J., He H., Peng J., Kung H. and Wong B.C.Y., Hypermethylation Status Around Exon 1 of XIAP-Associated Factor 1 in Human Gastric and Colon Cancer Cell Lines, Gastroenterology. 2004, 126 (4 Suppl 2): T981.

Researcher : Lo ACY

List of Research Outputs

Lo A.C.Y., Wu W.H., Chung S.S.M. and Chung S.K., Astrocytic endothelin-1 leads to blood-brain barrier disruption resulting in increased infarct, brain edema and more severe neurological deficits after experimental stroke, The Joint meeting - 2003 of the ISN (International Society for Neurochemistry) and the APSN (Asian-Pacific Society for Neurochemistry), Hong Kong, Aug 3-8. 2003.

Lo A.C.Y., Law L.P., Chung S.S.M. and Chung S.K., Endothelin-1 mediated signaling for increased blood-brain barrier breakdown and infarct after transient focal cerebral ischemia, Colloquium "Genomics and Proteomics", The 6^th Biennial Meeting of the APSN (Asian-Pacific Society for Neurochemistry), Feb 4-7. 2004.

Lo A.C.Y., Yaw L.P., Chung S.S.M. and Chung S.K., Over-expression of endothelin-1 in astrocytes leads to blood-brain barrier disruption, increased infarct, brain edema and more severe neurological deficits after experimental stroke, The 23^rd Scientific Meeting of the Hong Kong Society of Neurosciences, Hong Kong, People's Republic of China, Dec 9-10. 2003.

Lo A.C.Y., Yaw L.P., Chung S.S.M. and Chung S.K., Over-expression of endothelin-1 in astrocytes leads to blood-brain barrier disruption, increased infarct, brain edema and more severe neurological deficits after experimental stroke, The Eighth International Conference on Endothelin, Tsukuba, Japan, Nov 23-26. 2003.

Lo A.C.Y., Fung M.K.L., Au C.L., Chan T.S.K., Sauer B., Chung S.S.M. and Chung S.K., Transgenic mice over-expressing endothelin-1 in testis transactivated by a cre/loxP system showed decreased testicular capillary blood flow, Transgenic Research. 2004, 13: 119-134.

Researcher : Lum CT

List of Research Outputs

Peng Y., Yang P., Ng S.M., Lum C.T., Kung H. and Lin M.C., Protection of Xenopus laevis embryos against alcohol-induced delayed gut maturation and growth retardation by peroxiredoxin 5 and catalase, Journal of Molecular Biology. 2004, 340: 819-827.

Tu S., Jiang X., Lin M.C., Cui J., Yang Y., Lum C.T., Zou B., Zhu Y.B., Jiang S.H., Wong W.M., Chan O.O., Yuen M.F., Lam S.K., Kung H. and Wong B.C.Y., Suppression of Survivin Expression Inhibits in vivo Tumorigenicity and Angiogenesis in Gastric Cancer, Cancer Research. 2003, 63(22): 7724-7732.

Researcher : Ng SM

List of Research Outputs

Chen Y., Luk K.D.K., Cheung K.M.C., Lu W.W., An X., Ng S.M., Lin M.C. and Kung H., Combination of adeno-associated virus and adenovirus vectors expressing bone morphogenetic protein-2 produces enhanced osteogenic activity in immunocompetent rats, Biochemical and Biophysical Research Communications. 2004, 317(3): 675-681.

Lee M.Y., Yung W.H., Ng S.M., Chen L. and Chow B.K.C., Electrophysiological effects and release characteristics in the rat cerebellum, 6^th IBRO World congress of Neuroscience, Prague, Czech Republic. 2003.

Pang T.K.R., Lee T.O., Ng S.M., Yung W.H. and Chow B.K.C., CpG Methylation and Transcription Factors Sp1 and Sp3 Regulate the Expression of the Human Secretin Receptor Gene, Molecular Endocrinology. 2004, 18: 471-483.

Peng Y., Yang P., Ng S.M., Wong O.G., Liu J., He M.L., Kung H. and Lin M.C., A critical role of Pax6 in alcohol-induced fetal microcephaly, Neurobiology of Disease. 2004, 16: 370-376.

Peng Y., Yang P., Guo Y., Ng S.M., Liu J., Fung P.C.W., Tay D.K.C., Ge J., He M.L., Kung H. and Lin M.C., Catalase and peroxiredoxin 5 protect xenopus embryos against alcohol-induced ocular anomalies, Investigative Ophthalmology & Visual Science. 2004, 45 No. 1: 23-29.

Peng Y., Yang P., Ng S.M., Lum C.T., Kung H. and Lin M.C., Protection of Xenopus laevis embryos against alcohol-induced delayed gut maturation and growth retardation by peroxiredoxin 5 and catalase, Journal of Molecular Biology. 2004, 340: 819-827.

Siu K.Y., Sham M.H., Ng S.M. and Chow B.K.C., The Expression of Secretin in the Mouse Embryonic Development, 6^th IBRO World congress of Neuroscience, Prague, Czech Republic. 2003.

Researcher : Peng Y

List of Research Outputs

He M.L., Peng Y., Kung H. and Lin M.C., Inhibition of SARS-associated coronavirus infection and replication by RNA interference, 2003 US provisional patent No. 60/471, 922. 2003.

He M.L., Zheng B., Peng Y., Peiris J.S., Poon L.L., Yuen K.Y., Lin M.C., Kung H. and Guan Y., Inhibition of SARS-associated coronavirus infection and replication by RNA interference, Journal of the American Medical Association. 2003, 290(20): 2665-6.

Li X., Li G., Peng Y., Kung H. and Lin M.C., Suppression of Epstein-Barr virus-encoded latent membrane protein-1 by RNA interference inhibits the metastatic potential of nasopharyngeal carcinoma cells, Biochemical and Biophysical Research Communications. 2004, 315(1): 212-8.

Lin M.C., Peng Y. and Kung H., Catalase and peroxiredoxin 5 protect xenopus embryos against alcohol-induced ocular anomalies, 43^rd American Society for Cell Biology Annual Meeting, San Fancisco, CA, Dec 13-17. 2003.

Peng Y., Yang P., Ng S.M., Wong O.G., Liu J., He M.L., Kung H. and Lin M.C., A critical role of Pax6 in alcohol-induced fetal microcephaly, Neurobiology of Disease. 2004, 16: 370-376.

Peng Y., Kung H. and Lin M.C., A novel Xenopus laevis model to screen for molecular target and therapeutic drugs for alcohol-induced birth defects, including eye, brain, gut and growth retardation, US Provisional patent filed June 2004. 2004.

Peng Y., Yang P., Guo Y., Ng S.M., Liu J., Fung P.C.W., Tay D.K.C., Ge J., He M.L., Kung H. and Lin M.C., Catalase and peroxiredoxin 5 protect xenopus embryos against alcohol-induced ocular anomalies, Investigative Ophthalmology & Visual Science. 2004, 45 No. 1: 23-29.

Peng Y., Involvement of phosphatidylinositol-3 kinase signaling in neurogenesis during xenopus embryonic development, American Society for Cell Biology 43^rd Annual Meeting, San Francisco, USA, Dec 13-17, 2003. Molecular Biology of the Cell. 2003, 14: 515a.

Peng Y., Yang P., Ng S.M., Lum C.T., Kung H. and Lin M.C., Protection of Xenopus laevis embryos against alcohol-induced delayed gut maturation and growth retardation by peroxiredoxin 5 and catalase, Journal of Molecular Biology. 2004, 340: 819-827.

Researcher : Shi J

List of Research Outputs

Shi J., Zheng D., Man K., Fan S.T. and Xu R., TRAIL: a potential agent for cancer therapy, Current Molecular Medicine. 2003, 3(8): 727-736.

Researcher : Sze J

List of Research Outputs

Chen Y., Sze J. and He M.L., The occurrence of HBV cccDNA in the patients' sera is an indicator for HBV reactivation and an early signal of liver damage, World Journal of Gastroenterology. 2004, 10: 82-85.

Researcher : Tse LY

List of Research Outputs

Xu R., Sun X., Tse L.Y., Li H., Chan P.C., Xu S., Xiao W., Kung H., Krissansen G.W. and Fan S.T., Long-term expression of angiostatin suppresses metatatic liver cancer in mice (Abstract), The 5th Annual Scientific Meeting, Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 3 January 2004.

Researcher : Wong CM

List of Research Outputs

Ching Y.P., Leong V.Y.L., Wong C.M. and Kung H., Identification of an autoinhibitory domain of p21-activated protein kinase 5, Journal of Biological Chemistry. 2003, 278(36): 33621-33624.

Researcher : Wu WH

List of Research Outputs

Lo A.C.Y., Wu W.H., Chung S.S.M. and Chung S.K., Astrocytic endothelin-1 leads to blood-brain barrier disruption resulting in increased infarct, brain edema and more severe neurological deficits after experimental stroke, The Joint meeting - 2003 of the ISN (International Society for Neurochemistry) and the APSN (Asian-Pacific Society for Neurochemistry), Hong Kong, Aug 3-8. 2003.

Researcher : Xu R

Project Title:	Oral gene therapy of tumors by using recombinant AAV-TRAIL viral vectors
Investigator(s):	Dr. Xu R., Prof. Kung H.F.
Department:	Genome Research Centre
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	05/2002

Abstract:

To study tumor therapy by using recombinant AAV.

Project Title:	Peroral transduction of hepatocytes for diabetes gene therapy
Investigator(s):	Dr. Xu R., Prof. Lam K.S.L.
Department:	Genome Research Centre
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	10/2002

Abstract:

A major goal of gene therapy for Diabetes Mellitus (DM) is to restore long-term euglycemia. This study shall focus on clarifying the adeno-associated virus (AAV) vector transportation pathway from stomach to liver after oral administration. The research team will extend their previous study further to develop chimeric glucose- and insulin-sensitive promoters and insert them into the existing AAV vector system.

Project Title:	Preclinical study of lung cancer therapy using recombinant adeno-associate virus vector
Investigator(s):	Dr. Xu R.
Department:	Genome Research Centre
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	04/2003

Abstract:

To carrry out preclinical study of lung cancer therapy using recombinant adeno-associate virus vector.

Project Title:	Functional characterization of STAP gene and its potential use for gene therapy in liver cirrhosis, a leading cause of death and disability
Investigator(s):	Dr. Xu R., Prof. Fung P.C.W., Prof. Obara M., Prof. Yoshizato K.
Department:	Genome Research Centre
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	07/2003

Abstract:

To determine the effects of STAP on oxidative stress during the progress of liver cirrhosis; to identify the signaling pathway by which STAP affects oxidative stress; to determine the efficiency of STAP in scavenging of radical-derived organic peroxides; to establish a gene therapy animal model to prevent liver cirrhosisusing STAP as a therapeutic gene; to establish a gene therapy animal model to treat liver cirrhosis using STAP as a therapeutic gene.

List of Research Outputs

Cai K., Sham M.H., Zheng D.X. and Xu R., Anti-HBV drug and treatment, Chinese Journal of Medicine. 2003, 50: 744-746.

Cai K., Sham M.H., Tam P.K.H., Lam W.K. and Xu R., Lung cancer gene therapy, Gene Therapy and Molecular Biology. 2003, 7: 245-262.

Chen Y., Luk K.D.K., Cheung K.M., Xu R., Lin M.C., Lu W.W., Leong J.C. and Kung H., Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vectors, Gene Therapy. 2003, 10(16): 1345-53.

Cheung K.M.C., Chen Y., Luk K.D.K., Xu R., Lin M.C., Lu W.W., Leong J.C.Y. and Kung H., Delivery of bone morphogenetic proteins 2 anad 4 by use of adeno-associated viral gene therapy, Hong Kong Orthopaedic Association, Hong Kong, November 8-9, 2003.

Cheung K.M.C., Chen Y., Luk K.D.K., Kung H. and Xu R., Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vectors, 38th Annual Meeting of the Scoliosis Research Society, Quebec, Canada, September 10-13, 2003.

Kung H. and Xu R., Long term expression of angiostatin and suppression of liver metastatic cancer, Provisional patent no. 9661/048/888. 2004.

Lee K.W., Man K., Ho J.W.Y., Sun K.W., Ng T.P., Xu R. and Fan S.T., FTY720 selectively induced human hepatoma cell line apoptosis via Akt dephosphorylation (Abstract) , AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics, Boston, U.S.A., 17-21 November 2003.

Lee K.W., Man K., Ho J.W.Y., Sun K.W., Ng T.P., Ng I.O.L., Xu R. and Fan S.T., FTY720 selectively induces human hepatoma cell lines apoptosis via Akt dephosphorylation (Abstract), The 5th Annual Scientific Meeting, Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 3 January 2004.

Shi J., Zheng D., Man K., Fan S.T. and Xu R., TRAIL: a potential agent for cancer therapy, Current Molecular Medicine. 2003, 3(8): 727-736.

Xu R., A novel approach to treat liver metastesis cancer, 5^th China High Tech Fair, Shenzhen, China, Oct 12-17. 2003.

Xu R., Compositions and methods for preventing and treating liver cirrhosis, USA & China Regular Patent, May 5. 2004.

Xu R., Feasible strategies for cancer gene therapy, China Pharmaceutical University, Mar 8. 2004.

Xu R., Sun X., Tse L.Y., Li H., Chan P.C., Xu S., Xiao W., Kung H., Krissansen G.W. and Fan S.T., Long-term expression of angiostatin suppresses metatatic liver cancer in mice (Abstract), The 5th Annual Scientific Meeting, Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 3 January 2004.

Xu R., Cai K., Zheng D.X., Ma H., Xu S. and Fan S.T., Molecular therapeutics of HBV, Current Gene Therapy. 2003, 3 (4): 341-355.

Xu R. and Zheng D.X., National 863 annual report on lung cancer therapy, Ministry of Science and Technology, Beijing, China, Nov 18. 2003.

Xu R., Potential of gene pill for cancer therapy, International Society of Cancer Gene Therapy Singapore Conference, Feb 20-22. 2004.

Xu R., Sun X., Tse L.Y., Xiao W. and Krissansen G., Vaccination with adeno-associated-virus-transfected B7.1 tumor cells synergizes with angiostatin to eradicate disseminated liver metastases, The 7^th Meeting of the American Society of Gene Therapy, USA. 2004.

Xu R., rAAV-TRAIL for lung cancer therapy, USA & China Non-provisional Patent. 2004.

Xu R., Ma H., Liu Y., Kung H.K., Sun X. and Zheng D., rAAV-mediated TRAIL gene therapy eradicates liver metastatic tumors, Fifth Annual Scientific Meeting, Hong Kong, Jan 3. 2004.

Zheng D.X. and Xu R., National 863 annual report on liver cancer therapy, Ministry of Science and Technology, Beijing, China, Nov 18. 2003.

Researcher : Yang JY

Project Title:	The role of osmotic response element binding protein in osmoregulation
Investigator(s):	Dr. Yang J.Y.Q., Dr. Chung S.S.M., Dr. Ko C.B.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	11/2000

Abstract:

To generate transgenic mic overexpressing a dorminant negative form of OREBP; to evaluate the effects of down-regulating or blocking the transcription of osmotically-regulated genes.

Project Title:	The polyol pathway in agouti-induced lipogenesis and obesity
Investigator(s):	Dr. Yang J.Y.Q.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	10/2002
Completion Date:	09/2003

Abstract:

To establish the contribution of the polyol pathway to lipogenesis and/or adipogenesis; to determine whether the levels of blood free fatty acids and trilygcerides are reduced in agouti yellow mice deficient in AR or SD; to determine the de novo conversion of glucose to fatty acids and triglycerides in normal mice and mice deficient in AR; to determine if SD deficiency also reduce obesity in the agouti yellow mice; to determine if AR deficiency affects the activities of lipogenic/adipogenic transcriptional factors including ADD1/SREBP-1 and CHOP-C/EBP-a, anti obesity transcriptional factor FOXC2, and enzymes fatty acid synthase (FAS) and stearoyl Co-A desaturase (SCD); to determine if inhibitors of aldose reductase (ARI) would reduce obesity and improve dyslipidemia and glucose intolerance in the agouti yellow mice.

Project Title:	The polyol pathway as a thrifty pathway promoting energy storage
Investigator(s):	Dr. Yang J.Y.Q., Dr. Chung S.S.M.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	09/2003

Abstract:

To determine the in vivo conversion of glucose to fatty acids and triglycerides in normal mice and mice deficient in AR; to determine if AR deficiency affects the activities of lipogenic/adipogenic transcriptional factors including ADD1/SREBP-1 and CHOP-C/EBP-a, anti-obesity transcriptional factor FOXC2, and enzymes fatty acid synthase (FAS) and stearoyl CoA desaturase (SCD); to assess whether administration of inhibitors of aldose reductase (ARI) will affect obesity and improve glucose tolerance in the agouti yellow mice; to determine whether the effects of aldose reductase deficiency on agouti-induced lipogenesis and obesity can be duplicated in diet-induced obesity models.

Project Title:	The polyol pathway as a thrifty pathway promoting energy storage
Investigator(s):	Dr. Yang J.Y.Q., Dr. Chung S.S.M.
Department:	Institute of Molecular Biology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	09/2003

Abstract:

List of Research Outputs

Guo H., Wu X.C., Chau F.L.J., Tam S., Chung S.K., Chung S.S.M. and Yang J.Y., Aldose reductase local deficiency in the kidney is sufficient to impair urine concertrating mechanism, The 10^th SCBA 2004 Conference. 2004.

Lam A.K.M., Ko C.B., Tam S., Knepper M.A., Morris R., Yang J.Y., Chung S.K. and Chung S.S.M., Osmotic Response Element Binding Protein (OREBP) is essential for water reabsorption in the kidney, Experimental Biology 2004, Washington, DC, USA, April 17-21. 2004.

Lam A.K.M., Ko C.B., Tam S.C.F., Yang J.Y., Chung S.K. and Chung S.S.M., Osmotic Response Element Binding Protein is essential for water reabsorption in kidney, 8^th Research Postgraduate Symposium, Hong Kong, Dec 13. 2003.

Yang J.Y., Guo H., Wu X.C., Chau F.L.J., Tam S., Chung S.K. and Chung S.S.M., Aldose reductase deficiency in kidney collecting tubules impairs the urine concentrating mechanism, International Society of Nephrology 2004 Conference on Prevention of Progression of Rental disease. 2004.

Yang J.Y., Wu X.C., Chau F.L.J., Tam S., Oates P.J., Chung S.K. and Chung S.S.M., Blocking the polyol pathway protects diabetic mice against hypertriglyceridemia, American Diabetes Association 2004 Annual Meeting. 2004.

Researcher : Yang P

List of Research Outputs

Peng Y., Yang P., Ng S.M., Wong O.G., Liu J., He M.L., Kung H. and Lin M.C., A critical role of Pax6 in alcohol-induced fetal microcephaly, Neurobiology of Disease. 2004, 16: 370-376.

Peng Y., Yang P., Guo Y., Ng S.M., Liu J., Fung P.C.W., Tay D.K.C., Ge J., He M.L., Kung H. and Lin M.C., Catalase and peroxiredoxin 5 protect xenopus embryos against alcohol-induced ocular anomalies, Investigative Ophthalmology & Visual Science. 2004, 45 No. 1: 23-29.

Peng Y., Yang P., Ng S.M., Lum C.T., Kung H. and Lin M.C., Protection of Xenopus laevis embryos against alcohol-induced delayed gut maturation and growth retardation by peroxiredoxin 5 and catalase, Journal of Molecular Biology. 2004, 340: 819-827.

Researcher : Zhou Y

List of Research Outputs

Ren Y., He Q.Y., Fan J.Q., Li Z., Zhou Y., Chan H.M., Bacher M., Haab B., Chiu J.F., Vande Woude G. and Tam P.K.H., cDNA microarray and proteomic analysis of differential gene and protein expression in human neuroblastoma cells induced by macrophage migration inhibitory factor, The 50th Annual Congress of the British Association of Paediatric Surgeons, Estoril, Portugal, 15-18 July 2003.

-- End of Listing --